Evaluation of Potential Toxicity of Polyethylene Microplastics on Human Derived Cell Lines

Gautam, Ravi; Jo, JiHun; Acharya, Manju; Maharjan, Anju; Lee, DaEun; C, Pramod Bahadur K; Kim, ChangYul; Kim, KilSoo; Kim, Hyoung-Ah; Heo, Yong Seok

doi:10.2139/ssrn.4044450

Cited by 2 publications

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“…However, in our tested conditions, no increase of intracellular ROS levels was observed in treated cells after acute exposure to wMPs. A recent publication (Gautam et al, 2022) showed that PE MPs induce ROS in several cells line, with the exception of A549 and other epithelial cells, in accordance to our findings. Furthermore, recently, other authors (Jeon et al, 2021) have reported that the weathering process, such as UV radiation, increases the intrinsic ROS associated to MPs, but also the binding of serum proteins to MPs, which can act as a ROS scavenger.…”

Section: Discussionsupporting

confidence: 93%

Characterization of microparticles derived from waste plastics and their bio‐interaction with human lung A549 cells

Bengalli

Zerboni

Bonfanti

et al. 2022

J of Applied Toxicology

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Microplastics (MPs) represent a worldwide emerging relevant concern toward human and environmental health due to their intentional or unintentional release. Human exposure to MPs by inhalation is predicted to be among the most hazardous. MPs include both engineered, or primary MPs, and secondary MPs, materials obtained by fragmentation from any plastic good. The major part of the environmental MPs is constituted by the second ones that are irregular in size, shape and composition.These features make the study of the biological impact of heterogenous MPs of extremely high relevance to better estimate the real toxicological hazards of these materials on human and environmental organisms. The smallest fractions of plastic granules, relying on the micron-sized scale, can be considered as the most abundant component of the environmental MPs, and for this reason, they are typically used to perform toxicity tests using in vitro systems representative of an inhalation exposure scenario. In the present work, MPs obtained from industrial treatment of waste plastics (wMPs < 50 μm) were investigated, and after the physico-chemical characterization, the cytotoxic, inflammatory and genotoxic responses, as well as the modality of wMPs interactions with alveolar lung cells, were determined. Obtained results indicated that, at high concentrations (100 μg/ml) and prolonged exposure time (48 h), wMPs affect biological responses by inducing inflammation and genotoxicity, as a result of the cell-wMP interactions, also including the uptake of the smaller particles.

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Section: Discussionsupporting

confidence: 93%

Characterization of microparticles derived from waste plastics and their bio‐interaction with human lung A549 cells

Bengalli

Zerboni

Bonfanti

et al. 2022

J of Applied Toxicology

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“…The intestinal epithelium is composed of a diverse array of differentiated epithelial cells, including enterocytes, goblet cells, enteroendocrine cells, paneth cells, and microfold cells (Sinagoga and Wells, 2015), which not only play a primary role in nutrient digestion and absorption but also provide a protective barrier against physical, chemical, and biological damage through apical intercellular junctions (Chen et al, 2015). The interaction between M/NPs and human intestinal cells has been mainly studied using monocultures of human colorectal adenocarcinoma epithelial cell lines Caco-2 or HT29-MTX (Visalli et al, 2021;Gautam et al, 2022).…”

Section: Intestinal Oganoids For Toxicity Evaluation Of M/npsmentioning

confidence: 99%

Utilization of intestinal organoid models for assessment of micro/nano plastic-induced toxicity

Yuan,

Liu

2023

Front. Environ. Sci.

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Micro/nano plastics (M/NPs) are emerging pollutants that have extensively infiltrated various aspects of human life, posing a significant threat to the natural ecological systems. M/NPs can enter the digestive system through the oral cavity and accumulate in various organs. The current research on M/NPs primarily relies on model organisms, and there remains a dearth of direct evidence concerning the impact of M/NPs on human health. Commonly utilized specific two dimensional (2D) cultured cell lines exhibit substantial disparities in physiological functions when compared to multicellular tissues in vivo. The conduct of animal experiments is a time-consuming process, constrained by ethical considerations, and also confronted with interspecies variations. A significant breakthrough in biology is the development of organoids derived from stem cells. Intestinal organoids can mimic the complex structure and functionality of tissue, and can generate cell-cell and cell-matrix interactions that closely resemble physiological responses in the body. As a result, they provide a more accurate reflection of toxic effects and mechanisms, and hold great potential for applications in the environmental toxicology assessment. However, the current research on the toxic mechanisms of M/NPs using intestinal organoids is still in its early stages. The focus of this review is on the application of intestinal organoids in toxicology studies of M/NPs, assessing the correlation between M/NPs and diseases, as well as elucidating the molecular mechanisms underlying toxic effects. Ultimately, we present the challenges and potential solutions for utilizing intestinal organoids as models to evaluate M/NPs-induced toxicity, aiming to provide valuable insights for future research.

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Evaluation of Potential Toxicity of Polyethylene Microplastics on Human Derived Cell Lines

Cited by 2 publications

References 0 publications

Characterization of microparticles derived from waste plastics and their bio‐interaction with human lung A549 cells

Characterization of microparticles derived from waste plastics and their bio‐interaction with human lung A549 cells

Utilization of intestinal organoid models for assessment of micro/nano plastic-induced toxicity

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