Evaluation of preventive and therapeutic activity of novel non-steroidal anti-inflammatory drug, CG100649, in colon cancer: Increased expression of TNF-related apoptosis-inducing ligand receptors enhance the apoptotic response to combination treatment with TRAIL

Woo, Jong Kyu; Kang, Ju‐Hee; Jang, Yangsoo; Ro, Seonggu; Cho, Joong Myung; Kim, Hwan‐Mook; Lee, Sang-Jin; Oh, Seung Hyun

doi:10.3892/or.2015.3793

Cited by 14 publications

(11 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another recent study showed that DR4 and DR5 were upregulated while DcR1 and DcR2 downregulated in colon cancer cells after their treatment with a non-steroidal anti-inflammatory drug. Therefore, colon cancer cells became sensitive to TRAIL-induced apoptosis [107]. The fifth TRAIL receptor is osteoprotegerin (OPG), which is a secreted receptor for TRAIL with low affinity.…”

Section: Fas Cell Surface Death Receptor and Fas Ligandmentioning

confidence: 99%

Major apoptotic mechanisms and genes involved in apoptosis

et al. 2016

View full text Add to dashboard Cite

As much as the cellular viability is important for the living organisms, the elimination of unnecessary or damaged cells has the opposite necessity for the maintenance of homeostasis in tissues, organs and the whole organism. Apoptosis, a type of cell death mechanism, is controlled by the interactions between several molecules and responsible for the elimination of unwanted cells from the body. Apoptosis can be triggered by intrinsically or extrinsically through death signals from the outside of the cell. Any abnormality in apoptosis process can cause various types of diseases from cancer to auto-immune diseases. Different gene families such as caspases, inhibitor of apoptosis proteins, B cell lymphoma (Bcl)-2 family of genes, tumor necrosis factor (TNF) receptor gene superfamily, or p53 gene are involved and/or collaborate in the process of apoptosis. In this review, we discuss the basic features of apoptosis and have focused on the gene families playing critical roles, activation/inactivation mechanisms, upstream/downstream effectors, and signaling pathways in apoptosis on the basis of cancer studies. In addition, novel apoptotic players such as miRNAs and sphingolipid family members in various kind of cancer are discussed.

show abstract

Section: Fas Cell Surface Death Receptor and Fas Ligandmentioning

confidence: 99%

Major apoptotic mechanisms and genes involved in apoptosis

et al. 2016

View full text Add to dashboard Cite

show abstract

“…During this time, cells were treated twice a week with cell culture media containing 5 or 20 μg/mL ergosterol peroxide. The colonies measuring ≥30 μm were counted using a dissection microscope (Woo et al, 2015). Three independent experiments were performed with similar results; representative results of one experiment are presented.…”

Section: Anchorage-dependent and -Independent Colony Formation Assaymentioning

confidence: 99%

“…Frozen colon tissue sections were reacted with diluted primary antibodies for cyclin D1 (1:50), c-Myc (1:50), β-catenin (1:100) and Ki67 (1:1,000), and incubated with biotinylated goat anti-rabbit secondary antibody and avidin-biotin peroxidase. Sections were visualized by chromogen 3,3'-diaminobenzidine (Dako, K3466) and counterstained with hematoxylin (Woo et al, 2015). The intensity and localization of immunoreactivity against antibodies was viewed under a light microscope (200×) by Aperio ScanScope (Aperio Technologies, CA, USA).…”

Section: Histological Analysismentioning

confidence: 99%

Ergosterol peroxide from Chaga mushroom (Inonotus obliquus) exhibits anti-cancer activity by down-regulation of the β-catenin pathway in colorectal cancer

Kang

Jang

Mishra

et al. 2015

Journal of Ethnopharmacology

Self Cite

116

View full text Add to dashboard Cite

“…The significant upregulation of TRAIL DR4 and DR5 may be attributed to the fact that the increased expression of TRAIL receptors may enhance the apoptotic response induced by TRAIL and lead to an increase in the apoptotic rate among tumor cells [18] [19]. These results were supported by the positive correlation between the expressions of TRAIL DR4 and the apoptotic index in the breast cancer tissues.…”

Section: Discussionmentioning

confidence: 82%

TRAIL and TRAIL Receptors as Prognostic Markers in Breast Cancer Patients

Ibrahim¹,

Abouelenein²,

Sakr³

et al. 2019

AJMB

View full text Add to dashboard Cite

Background: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a key player in the extrinsic pathway of apoptosis; it selectively damages cancer cells through binding to its surface receptors, however, cancers can escape this pathway through expression of dysfunctional decoy receptors. Purpose: The present study directed mainly to elucidate the serum TRAIL levels in breast cancer patients and to explore the variation in gene expression of TRAIL death and decoy receptors in breast cancer tissues, and to explore their role as prognostic markers in breast cancer as well as to detect their correlation with Patients' Clinical Characteristics. Subjects and Methods: TRAIL levels were assayed in the sera of 124 breast cancer patients and 150 healthy females. Moreover, the expression of TRAIL death and decoy receptors was determined in both malignant and adjacent normal breast tissues collected from patients. ER, PR and Her-2 expression in breast cancer tissue were performed using immunohistochemical method. Apoptotic index (AI) was analyzed using H&E stain under light microscopy. Results: Serum levels of TRAIL in breast cancer patients were significantly lower than controls (P < 0.001), additionally, the expression of DR4, DR5 and DcR1 were significantly up-regulated (p < 0.001, p < 0.001, p = 0.039, respectively), however, no significant difference was found in the expression of DcR2 in breast cancer tissues as compared to the corresponding normal tissues. Moreover, the apoptotic index in breast cancer tissues was significantly higher than the corresponding normal tissues. On the other side, decreased Serum TRAIL levels and increased DcR1 expression were associated with decreased overall patients' survival. Conclusions: The expression of both DR4 and DR5 is required for TRAILinduced apoptosis in breast cancer tissues; in addition, serum TRAIL and pro-How to cite this paper: Ibrahim

show abstract

Evaluation of preventive and therapeutic activity of novel non-steroidal anti-inflammatory drug, CG100649, in colon cancer: Increased expression of TNF-related apoptosis-inducing ligand receptors enhance the apoptotic response to combination treatment with TRAIL

Cited by 14 publications

References 26 publications

Major apoptotic mechanisms and genes involved in apoptosis

Major apoptotic mechanisms and genes involved in apoptosis

Ergosterol peroxide from Chaga mushroom (Inonotus obliquus) exhibits anti-cancer activity by down-regulation of the β-catenin pathway in colorectal cancer

TRAIL and TRAIL Receptors as Prognostic Markers in Breast Cancer Patients

Contact Info

Product

Resources

About