Evaluation of Pro/Antioxidant Imbalance in Blood of Women with Polycystic Ovary Syndrome Based on Determination of Oxidized Low-Density Lipoproteins and Ferric Reducing Ability of Plasma Values

Abstract

We investigated selected pro/antioxidant parameters in a group of women with polycystic ovary syndrome (PCOS) divided according to age, body mass index (BMI), waist-to-hip ratio (WHR), homeostatic model assessment for insulin resistance (HOMA-IR) and quantitative insulin sensitivity check index (Quicki). We chose oxidized low-density lipoproteins (oxLDL-C) as a marker of oxidative stress and the ferric reducing ability of plasma (FRAP) as a marker of antioxidant status. In women with PCOS, the values of BMI, WHR, age and concentration of glucose significantly affected oxLDL-C concentration and FRAP values. FRAP correlated with oxLDL-C level in the whole group and in women who were insulin sensitive (HOMA-IR < 2.0). There was a negative relationship between the concentration of Anti-Müllerian hormone and both oxLDL-C and FRAP. Furthermore, the value of FRAP was inversely correlated with luteinizing hormone (LH), follicle-stimulating hormone (FSH) and androstenedione, whereas it was positively correlated with the LH/FSH ratio. The concentration of oxLDL and the value of FRAP are significantly associated with selected metabolic and hormonal parameters in the course of PCOS.

Antioxidant Defense Expressed as Glutathione Status and Keap1-Nrf2 System Action in Relation to Anthropometric Parameters and Body Composition in Young Women with Polycystic Ovary Syndrome

“…Finally, the lack of lipid profile and inflammatory markers may be a further limitation of our study. However, it is well known that patients with PCOS often have abnormal lipid levels, and chronic low-grade systemic inflammation is an important factor in this disease [ 6 , 14 , 33 , 43 , 44 ].…”

“…Polycystic ovary syndrome (PCOS) is one of the most common endocrinopathies and its prevalence—depending on the adopted diagnostic criteria and the population studied—ranges from 4 to 16% of reproductive-age women [ 1 , 2 , 3 ]. Characteristic features of the syndrome include menstrual disorders, hyperandrogenism, and often obesity and infertility [ 4 , 5 , 6 ]. In addition to primary and secondary infertility, pregnancy complications are more common in this patient group, including gestational diabetes, hypertension, preeclampsia, and a higher risk of miscarriage or low-birth-weight offspring [ 7 , 8 , 9 ].…”

Evaluation of Selected Oxidant/Antioxidant Parameters in Patients with Relapsing-Remitting Multiple Sclerosis Undergoing Disease-Modifying Therapies

“…FRAP was measured in diluted serum (10-fold in PBS) using the colorimetric method with ferric tripyridyltriazine described earlier [ 30 ]. The FRAP reagent was prepared by mixing 300 mM of acetate buffer, with a pH of 3.6, with 10 mM of 2,4,6-tripyridyl-s-triazine (TPTZ) (Cat.…”

Molecular Research on Polycystic Ovary Syndrome (PCOS)

“…They proposed AIP and Castelli Index as atherogenicity additional indicators to assess the risk of cardiometabolic diseases in PCOS in order to rapidly identify risk groups among this population. Niepsuj et al [15] took into account further parameters, such as oxidized low-density lipoproteins (oxLDL-C) and the ferric-reducing ability of plasma (FRAP), to evaluate pro/anti antioxidant imbalance blood in PCOS patients. This clinical study found a significant association between oxLDL concentration and the FRAP value, and selected hormonal and metabolic parameters in the PCOS course.…”