Evaluation of probiotic characteristics and whole genome analysis of Bacillus velezensis R-71003 isolated from the intestine of common carp (Cyprinus carpio L.) for its use as a probiotic in aquaculture

Kang, Meiru; Su, Xi; Yun, Lili; Shen, Yifeng; Feng, Junchang; Yang, Guokun; Meng, Xiaolin; Zhang, Jianxin; Chang, Xulu

doi:10.1016/j.aqrep.2022.101254

Cited by 12 publications

(6 citation statements)

References 44 publications

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“…These four isolated strains, when stained with Congo red solution, produced clear hydrolysis circles around the colonies, the hydrolysis diameters are 4.82 ± 0.13, 7.92 ± 0.16, 26.20 ± 0.44, and 11.56 ± 0.45 mm, respectively, indicating that they have cellulose hydrolysis ability ( Table 1 ). Cellulose-degrading bacteria were screened based on the HCR ratio ( Kang et al, 2022 ), and the hydrolysis ratios of the four isolated strains in the two media were compared. The hydrolysis ratios of each strain were 1.50 ± 0.07, 1.36 ± 0.11, 3.61 ± 0.05, and 3.57 ± 0.32, respectively.…”

Section: Resultsmentioning

confidence: 99%

Screening and genome-wide analysis of lignocellulose-degrading bacteria from humic soil

et al. 2023

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Crop straw contains huge amounts of exploitable energy, and efficient biomass degradation measures have attracted worldwide attention. Mining strains with high yields of cellulose-degrading enzymes is of great significance for developing clean energy and industrial production of related enzymes. In this study, we reported a high-quality genome sequence of Bacillus velezensis SSF6 strain using high-throughput sequencing technology (Illumina PE150 and PacBio) and assessed its lignocellulose degradation potential. The results demonstrated that the genome of B. velezensis SSF6 was 3.89 Mb and contained 4,015 genes, of which 2,972, 3,831 and 158 genes were annotated in the COGs (Clusters of Orthologous Groups), KEGG (Kyoto Encyclopedia of Genes and Genomes) and CAZyme (Carbohydrate-Active enZymes) databases, respectively, and contained a large number of genes related to carbohydrate metabolism. Furthermore, B. velezensis SSF6 has a high cellulose degradation capacity, with a filter paper assay (FPA) and an exoglucanase activity of 64.48 ± 0.28 and 78.59 ± 0.42 U/mL, respectively. Comparative genomic analysis depicted that B. velezensis SSF6 was richer in carbohydrate hydrolase gene. In conclusion, the cellulose-degrading ability of B. velezensis SSF6 was revealed by genome sequencing and the determination of cellulase activity, which laid a foundation for further cellulose degradation and bioconversion.

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Section: Resultsmentioning

confidence: 99%

Screening and genome-wide analysis of lignocellulose-degrading bacteria from humic soil

et al. 2023

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“…The isolation and culturing of B. velezensis isolate from the gill specimens exhibited rapid and robust proliferation in the laboratory setting, thus designating this particular isolate for subsequent analysis. Using 16SrRNA sequencing and construction of a phylogenetic tree using MEGA11 software, the relatedness of the species in question with other members of the Bacillus genus was determined (Liu et al 2017;Kang et al 2022). Instead of assessing the capacity for xenobiotic degradation through time-consuming and laborious in vitro techniques, the focus shifted towards the Using the methods employed, genomic data is relegated to a supplementary role rather than a primary one.…”

Section: Discussionmentioning

confidence: 99%

“…1). Typically, such resemblance is addressed through comprehensive genome sequencing (Liu et al 2017;Kang et al 2022), since these species of the Bacillus genus are genetically related by almost 99%.…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Bioinformatic insights into the xenobiotic degradation potential gene clusters of fish-associated novel Bacillus velezensis SNR14-4

Madhavan,

Dinesh,

Muhammed

et al. 2024

EEB

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Bacillus velezensis is a member of the genus Bacillus, which harbours useful, novel, and efficient secondary metabolites that can be utilized in the disruption of xenobiotics. Although a few strains of B. velezensis and related species are reported every year as having xenobiotic metabolism potential, several novel gene clusters are still unexplored, which could be more potent than those already discovered. The current B. velezensis strain was isolated from gills of healthy Oreochromis niloticus and the novelty of the strain was assessed through whole genome sequence analysis. Prokka, DFAST, BAKTA, and RASTtk were computational tools utilized for genome elucidation following the genome assembly. Protein and protein pathway prediction was achieved through the PATRIC database. Additionally, the resistance genes against microorganisms were examined through CARD (via Proksee), bacteriocin, and RiPPs using BAGEL4, and forecast of virulence factors using VFDB in PATRIC. The analysis led to identification of indicator genes for xenobiotic breakdown. The results were compared to pre-existing strains of B. velezensis and it was compelling to conclude the high biotechnological potential and the candidacy of the strain in xenobiotic degradation.

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“…The application of thermotolerant probiotics that can withstand stress during the processing and storage of products in aquaculture is of great interest [ 48 ]. High temperature resistance test performed on B. velezensis showed high survival rate of the strain after exposure to 80 °C for 2, 5, and 10 min [ 49 ]. Likewise, Bacillus methylotrophicus LSG2-3-2 and Bacillus tequilensis LSG3-6 could also tolerate high temperatures up to 80 °C [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

“…Gradual increase of B. amyloliquefaciens isolate within pH range from 1 to 7 followed by decrease in growth from pH 7 to 10, which showed the ability of isolates to survive in extreme acidic and alkaline condition [ 20 ]. More than 50% of isolate B. velezensis survived after the exposure to pH 2, 3, 4, and 5 [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Single and Multi-Strain Probiotics on In Vitro Strain Compatibility, Pathogen Inhibition, Biofilm Formation Capability, and Stress Tolerance

Puvanasundram

Chong

Sabri

et al. 2022

Biology

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Compatibility of each strain in a multi-strain probiotic (MSP), along with its properties, becomes a strong base for its formulation. In this study, single-strain probiotics (SSPs) and multi-strain probiotics (MSPs) were evaluated in vitro for strain compatibility, microbial antagonism, biofilm formation capacity, and stress tolerance. Bacillus amyloliquefaciens L11, Enterococcus hirae LAB3, and Lysinibacillus fusiformis SPS11 were chosen as MSP1 candidates because they showed much stronger antagonism to Aeromonas hydrophila and Streptococcus agalactiae than a single probiotic. MSP 2 candidates were Lysinibacillus fusiformis strains SPS11, A1, and Lysinibacillus sphaericus strain NAS32 because the inhibition zone produced by MSP 2 against Vibrio harveyi and Vibrio parahaemolyticus was much higher than that produced by its constituent SSPs. MSP1 in the co-culture assay reduced (p < 0.05) A. hydrophila count from 9.89 ± 0.1 CFU mL−1 to 2.14 ± 0.2 CFU mL−1. The biofilm formation of both MSPs were significantly higher (p < 0.05) than its constituent SSPs and the pathogens. The SSPs in both MSPs generally showed resistance to high temperatures (80, 90, and 100 °C) and a wide range of pH (2 to 9). This in vitro assessment study demonstrates that MSP1 and 2 have the potential to be further explored as multi-strain probiotics on selected aquatic species.

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Evaluation of probiotic characteristics and whole genome analysis of Bacillus velezensis R-71003 isolated from the intestine of common carp (Cyprinus carpio L.) for its use as a probiotic in aquaculture

Cited by 12 publications

References 44 publications

Screening and genome-wide analysis of lignocellulose-degrading bacteria from humic soil

Screening and genome-wide analysis of lignocellulose-degrading bacteria from humic soil

Bioinformatic insights into the xenobiotic degradation potential gene clusters of fish-associated novel Bacillus velezensis SNR14-4

Efficacy of Single and Multi-Strain Probiotics on In Vitro Strain Compatibility, Pathogen Inhibition, Biofilm Formation Capability, and Stress Tolerance

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