Evaluation of Probiotic Properties and Prebiotic Utilization Potential of Weissella paramesenteroides Isolated From Fruits

Pabari, Kinjal; Pithva, Sheetal; Kothari, Charmy; Purama, Ravi Kiran; Kondepudi, Kanthi Kiran; Vyas, B. R. M.; Kothari, Ramesh; Ambalam, Padma

doi:10.1007/s12602-019-09630-w

Cited by 45 publications

(29 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Weissella is a genus of lactic acid bacteria (LAB) consisting of species formerly included in the Leuconostoc paramesenteroides group and commonly found in fermented foods, milk, vegetables, feces, environment, and vertebrates including humans ( Du et al, 2018 ; Panthee et al, 2019 ). Several strains, mostly belonging to the species Weissella cibaria , Weissella confusa , and Weissella paramesenteroides , have been widely investigated for probiotic characteristics ( Tanizawa et al, 2014 ; Xia et al, 2019 ; Pabari et al, 2020 ) and for their capacity to protect against bacterial and fungal infection ( Li et al, 2017 ; Wang et al, 2017 ). Some strains showed effectiveness in maintaining host health due to their anti-toxicity, antitumor activity, and immunomodulatory properties ( Hong et al, 2016 ; Ojekunle et al, 2017 ; Yu et al, 2018 , 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Next-Generation Sequencing for Whole-Genome Characterization of Weissella cibaria UTNGt21O Strain Originated From Wild Solanum quitoense Lam. Fruits: An Atlas of Metabolites With Biotechnological Significance

Tenea

Hurtado

2021

Front. Microbiol.

View full text Add to dashboard Cite

The whole genome of Weissella cibaria strain UTNGt21O isolated from wild fruits of Solanum quitoense (naranjilla) shrub was sequenced and annotated. The similarity proportions based on the genus level, as a result of the best hits for the entire contig, were 54.84% with Weissella, 6.45% with Leuconostoc, 3.23% with Lactococcus, and 35.48% no match. The closest genome was W. cibaria SP7 (GCF_004521965.1) with 86.21% average nucleotide identity (ANI) and 3.2% alignment coverage. The genome contains 1,867 protein-coding genes, among which 1,620 were assigned with the EggNOG database. On the basis of the results, 438 proteins were classified with unknown function from which 247 new hypothetical proteins have no match in the nucleotide Basic Local Alignment Search Tool (BLASTN) database. It also contains 78 tRNAs, six copies of 5S rRNA, one copy of 16S rRNA, one copy of 23S rRNA, and one copy of tmRNA. The W. cibaria UTNGt21O strain harbors several genes responsible for carbohydrate metabolism, cellular process, general stress responses, cofactors, and vitamins, conferring probiotic features. A pangenome analysis indicated the presence of various strain-specific genes encoded for proteins responsible for the defense mechanisms as well as gene encoded for enzymes with biotechnological value, such as penicillin acylase and folates; thus, W. cibaria exhibited high genetic diversity. The genome characterization indicated the presence of a putative CRISPR-Cas array and five prophage regions and the absence of acquired antibiotic resistance genes, virulence, and pathogenic factors; thus, UTNGt21O might be considered a safe strain. Besides, the interaction between the peptide extracts from UTNGt21O and Staphylococcus aureus results in cell death caused by the target cell integrity loss and the release of aromatic molecules from the cytoplasm. The results indicated that W. cibaria UTNGt21O can be considered a beneficial strain to be further exploited for developing novel antimicrobials and probiotic products with improved technological characteristics.

show abstract

Section: Introductionmentioning

confidence: 99%

Next-Generation Sequencing for Whole-Genome Characterization of Weissella cibaria UTNGt21O Strain Originated From Wild Solanum quitoense Lam. Fruits: An Atlas of Metabolites With Biotechnological Significance

Tenea

Hurtado

2021

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…In order to define a carbohydrate as a prebiotic, in addition to being fermented by microorganisms, the compound should exhibit resistivity towards mammalian gastric enzymes and the acidity of the stomach [5]. On the other hand, prebiotics must be susceptible to the enzymes synthesized by bacteria residing in the colon [6]. What makes them different from other dietary fiber, such as xylans, and cellulose is that prebiotics favor the growth of only the beneficial microorganisms [7].…”

Section: Introductionmentioning

confidence: 99%

The In Vitro Analysis of Prebiotics to Be Used as a Component of a Synbiotic Preparation

Śliżewska

Chlebicz-Wójcik

2020

Nutrients

View full text Add to dashboard Cite

Prebiotics are food components that are selectively fermented by beneficial microbiota and which confer a health benefit. The aim of the study was to select a prebiotic for the chosen probiotic strains to create a synbiotic. The impact of prebiotics (inulin, maltodextrin, corn starch, β-glucan, and apple pectin) on five Lactobacillus spp. strains’ growth and metabolites synthesis (lactic, acetic, propionic, and butyric acids, ethanol, and acetaldehyde) was tested by the plate count method and by high-performance liquid chromatography, respectively. Moreover, the differences in the ratio of D(−) and L(+) lactate isomers produced by Lactobacillus spp., as well as variations in the probiotics’ enzymatic profiles associated with the prebiotic used for cultivation, were determined with a Megazyme rapid assay kit and API® ZYM assay, accordingly. Finally, the influence of the carbon source (prebiotic) used on the antagonistic activity of the probiotic strains towards pathogenic bacteria, such as Salmonella spp. or Listeria monocytogenes was analyzed in the co-cultures. The results showed that the growth, metabolic profile, and antagonistic activity of the probiotics towards selected pathogens were the most favorable when 2% (w/v) of inulin was used. Therefore, the combination of inulin with selected probiotics is a promising synbiotic mixture.

show abstract

“…has only recently been investigated in humans as well as in animals. Several studies have reported Weissella strains, mostly belonging to the species Weissella cibaria, Weissella confusa and Weissella paramesenteroides, to possess some functional properties relevant to their use as probiotics [3][4][5][6]. Notably, W. cibaria JW15 was found to possess anti-oxidant and anti-inflammatory properties in vitro [7,8], and to exert immune-modulatory effects in mice [9] and in human subjects [10].…”

Section: Introductionmentioning

confidence: 99%

Integrated Phenotypic-Genotypic Analysis of Candidate Probiotic Weissella Cibaria Strains Isolated from Dairy Cows in Kuwait

Patrone

Al-Surrayai

Romaniello

et al. 2020

Probiotics & Antimicro. Prot.

View full text Add to dashboard Cite

Probiotics represent a possible strategy for controlling intestinal infections in livestock. Members of the Weissella genus are increasingly being studied for health-related applications in animals and humans. Here we investigated the functional properties of two Weissella cibaria strains isolated from cows reared in Kuwait breeding facilities by combining phenotypic with genomic analyses. W. cibaria SP7 and SP19 exhibited good growth in vitro under acidic conditions and in the presence of bile salts compared to the reference probiotic Lacticaseibacillus (formerly Lactobacillus) rhamnosus GG. Both strains were able to adhere to Caco-2 and HT-29 cell lines, as well as to mucin. The cell-free supernatants of the two isolates exhibited inhibitory activity towards Escherichia coli ATCC 25,922 and Salmonella enterica UC3605, which was ultimately due to the low pH of supernatants. W. cibaria SP19 showed a co-aggregation ability similar to that of L. rhamnosus GG when incubated with S. enterica. Whole genome sequencing and analysis revealed that both strains harbored several genes involved in carbohydrate metabolism and general stress responses, indicating bacterial adaptation to the gastrointestinal environment. We also detected genes involved in the adhesion to host epithelial cells or extracellular matrix. No evidence of acquired antibiotic resistance or hemolytic activity was found in either strain. These findings shed light on the potential of W. cibaria for probiotic use in livestock and on the mechanisms underlying host-microbe interaction in the gut. W. cibaria` strain SP19 exhibited the best combination of in vitro probiotic properties and genetic markers, and is a promising candidate for further investigation.

show abstract

Evaluation of Probiotic Properties and Prebiotic Utilization Potential of Weissella paramesenteroides Isolated From Fruits

Cited by 45 publications

References 42 publications

Next-Generation Sequencing for Whole-Genome Characterization of Weissella cibaria UTNGt21O Strain Originated From Wild Solanum quitoense Lam. Fruits: An Atlas of Metabolites With Biotechnological Significance

Next-Generation Sequencing for Whole-Genome Characterization of Weissella cibaria UTNGt21O Strain Originated From Wild Solanum quitoense Lam. Fruits: An Atlas of Metabolites With Biotechnological Significance

The In Vitro Analysis of Prebiotics to Be Used as a Component of a Synbiotic Preparation

Integrated Phenotypic-Genotypic Analysis of Candidate Probiotic Weissella Cibaria Strains Isolated from Dairy Cows in Kuwait

Contact Info

Product

Resources

About