Contemporary farming and competitive market conditions are forcing nutritionists and physiologists to seek unconventional ways to upgrade rabbits' performance. Among these, feed additives such as antibiotics, probiotics, prebiotics, and immune modulators might be a good alternative to increase weight gain, improve feed efficiency, superior physiological functions of the body, and boost immune responses.Chitosan is one of the natural feed additives. It is derived from chitin, a polysaccharide formed by N-acetyl-D glucosamine units found in insects, marine diatoms, algae, fungi, and crustacea, through deacetylation, demineralization deproteinization, and decolouration (Synowiecki & Al-Khateeb, 2003;Abd El-Hack et al., 2020Abd El-Aziz et al., 2022). Previous studies have shown that chitosan as a dietary supplement in animal feed improved the digestive system performance by absorbing accumulated toxins and treating chronic constipation and gastrointestinal ulcers. In addition, it reduced the absorption of cholesterol from the diet. Therefore, in the future, chitosan may have wider uses, such as a feed additive, to improve the productive and reproductive performance of