Evaluation of protection induced by immunisation of domestic pigs with deletion mutant African swine fever virus BeninΔMGF by different doses and routes

Sánchez-Cordón, P.J.; Jabbar, Tamara; Berrezaie, Margot; Chapman, Dave; Reis, Ana Luísa; Sastre, Patricia; Rueda, Paloma; Goatley, Lynnette; Dixon, Linda K.

doi:10.1016/j.vaccine.2017.12.030

Cited by 60 publications

(72 citation statements)

References 24 publications

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“…Similar to previous results (22,24), the virus genome was detected in blood samples from all animals immunized with BeninΔMGF (group B) at days 14, 24, and 52 p.i. Viremia levels decreased progressively from day 14 p.i.…”

Section: Resultssupporting

confidence: 89%

“…Single immunizations with BeninΔMGF have not been tested before. However, even lower doses (10 2 and 10 3 TCID 50 ) administered twice by the intramuscular route over an interval of 3 weeks were able to induce up to 100% protection (22,24) when animals were challenged shortly (18 to 21 days) after the boost immunization. On the basis of these results and with the objective of performing a comparative evaluation, both vaccine candidates were selected to test in parallel their capacity to induce long-term protection after a single dose (10 4 TCID 50 ) was administered by the intramuscular route, considered the most feasible and safest route for immunizations.…”

Section: Importancementioning

confidence: 96%

“…in which domestic pigs were immunized with BeninΔMGF or OURT88/3 (22)(23)(24), all immunized pigs that did not become protected showed an increase in IL-10 serum concentrations just before euthanasia. In a previous short-term study, it was suggested that ␥␦-T cells and IL-10 levels could be related to mortality (62).…”

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confidence: 99%

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Absence of Long-Term Protection in Domestic Pigs Immunized with Attenuated African Swine Fever Virus Isolate OURT88/3 or BeninΔMGF Correlates with Increased Levels of Regulatory T Cells and Interleukin-10

Sánchez-Cordón

Jabbar

Chapman

et al. 2020

J Virol

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Following short immunization protocols, naturally attenuated African swine fever virus (ASFV) isolate OURT88/3 and deletion mutant BeninΔMGF have previously been shown to induce high percentages of protection in domestic pigs against challenge with virulent virus. The results obtained in the present study show that a single intramuscular immunization of domestic pigs with OURT88/3 or BeninΔMGF followed by a challenge with the virulent Benin 97/1 isolate at day 130 postimmunization did not trigger the mechanisms necessary to generate immunological memory able to induce long-term protection against disease. All pigs developed acute forms of acute swine fever (ASF). Gamma interferon-producing cells peaked at day 24 postimmunization, declining thereafter. Surprisingly, the levels of regulatory T cells (Tregs) and interleukin-10 (IL-10) were elevated at the end of the experiment, suggesting that regulatory components of the immune system may inhibit effective protection. IMPORTANCE The duration of immunity for any vaccine candidate is crucial. In the case of African swine fever virus vaccine candidates, this issue has received little attention. Attenuated viruses have proven protective following short immunization protocols in which pigs were challenged a few weeks after the first immunization. Here, the duration of immunity and the immune responses induced over a duration of 130 days were studied during prechallenge and after challenge of pigs immunized with the naturally attenuated isolate OURT88/3 and an attenuated gene-deleted isolate, BeninΔMGF. After a single intramuscular immunization of domestic pigs with the OURT88/3 isolate or BeninΔMGF virus, animals were not protected against challenge with the virulent Benin 97/1 ASFV genotype I isolate at day 130 postimmunization. The levels of regulatory T cells and IL-10 were elevated at the end of the experiment, suggesting that regulatory components of the immune system may inhibit effective protection.

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Section: Resultssupporting

confidence: 89%

Section: Importancementioning

confidence: 96%

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Absence of Long-Term Protection in Domestic Pigs Immunized with Attenuated African Swine Fever Virus Isolate OURT88/3 or BeninΔMGF Correlates with Increased Levels of Regulatory T Cells and Interleukin-10

Sánchez-Cordón

Jabbar

Chapman

et al. 2020

J Virol

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“…A new generation of ASFV gene-deletion mutants have been generated that have induced protective responses in pigs ( O’Donnell et al, 2015a , Reis et al, 2016 , O’Donnell et al, 2017 , Monteagudo et al, 2017 , Sánchez-Cordón et al, 2017b ). Cross-protection against a genotype II isolate was induced by a genotype I attenuated virus, suggesting that cross-protective attenuated virus vaccines may be feasible.…”

Section: Development Of Vaccines Against African Swine Fever Virusmentioning

confidence: 99%

African swine fever: A re-emerging viral disease threatening the global pig industry

Sánchez-Cordón

Montoya

Reis

et al. 2018

The Veterinary Journal

366

327

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HighlightsGenotype II African swine fever virus (ASFV) circulating in Europe has high pathogenicity for domestic pigs and wild boar.Genotype II ASFV strains in Europe do not exhibit attenuation and have limited ability to establish persistent infections.The wild boar population in Eastern Europe plays an important role in the maintenance and spread of ASFV.Immune responses against ASFV are not fully understood, hampering the rational design of vaccines.Control relies on ‘stamping-out’ policies and control of pig movements, which have a significant impact on affected regions.

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“…Having noted the drawbacks of in vitro attenuated ASFVs, target gene‐deleted live attenuated and subunit vaccines become attractive options, and as a result, several gene‐deleted ASF vaccines have been attempted (Gallardo, Sánchez, et al, ; Monteagudo et al, ; O’Donnell et al, , ; Reis et al, ). By targeted deletion of virulence‐related genes, it has been shown that it would be possible to define the balance between attenuation and immunogenicity (Reis et al, ; Sánchez‐Cordón, Jabbar, et al, ). To date, several virulence‐associated ASFV genes such as 9GL, UK, CD2v, DP148R and MGFs have been deleted to generate attenuated ASF vaccines.…”

Section: Asf Vaccine Strategies Have Evolved Through Different Scenariosmentioning

confidence: 99%

Current status and evolving approaches to African swine fever vaccine development

Teklue

Sun

Abid

et al. 2019

Transbound Emerg Dis

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African swine fever (ASF) is a highly lethal haemorrhagic disease of swine caused by African swine fever virus (ASFV), a unique and genetically complex virus. The disease continues to be a huge burden to the pig industry in Africa, Europe and recently in Asia, especially China. The purpose of this review was to recapitulate the current scenarios and evolving trends in ASF vaccine development. The unavailability of an applicable ASF vaccine is partly due to the complex nature of the virus, which encodes various proteins associated with immune evasion. Moreover, the incomplete understanding of immune protection determinants of ASFV hampers the rational vaccine design. Developing an effective ASF vaccine continues to be a challenging task due to many undefined features of ASFV immunobiology. Recent attempts on DNA and live attenuated ASF vaccines have been reported with promising efficacy, and especially live attenuated vaccines have been proved to provide complete homologous protection. Single‐cycle viral vaccines have been developed for various diseases such as Rift Valley fever and bluetongue, and the rational extension of these strategies could be helpful for developing single‐cycle ASF vaccines. Therefore, live attenuated vaccines in short term and single‐cycle vaccines in long term would be the next generation of ASF vaccines.

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Evaluation of protection induced by immunisation of domestic pigs with deletion mutant African swine fever virus BeninΔMGF by different doses and routes

Cited by 60 publications

References 24 publications

Absence of Long-Term Protection in Domestic Pigs Immunized with Attenuated African Swine Fever Virus Isolate OURT88/3 or BeninΔMGF Correlates with Increased Levels of Regulatory T Cells and Interleukin-10

Absence of Long-Term Protection in Domestic Pigs Immunized with Attenuated African Swine Fever Virus Isolate OURT88/3 or BeninΔMGF Correlates with Increased Levels of Regulatory T Cells and Interleukin-10

African swine fever: A re-emerging viral disease threatening the global pig industry

Current status and evolving approaches to African swine fever vaccine development

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