“…For detection of mRNA for metabolic genes commercially available TaqMan probes (Applied Biosystems, Waltham, Massachusetts, USA) dedicated to mouse tissue were used (see Supporting Information, Table S2). The level of mRNA for glycogen synthase ( Gys1 ) was used as reference (Kim et al, ). In contrast to Gys1 , our proteomic data revealed that the expression of all commonly used in qRT‐PCR referential genes (Boda, Pini, Hoxha, Parolisi, & Tempia, ) changed during aging or they were expressed at very low levels (Supporting Information, Table S3).…”