Evaluation of pyrene and tetracosane degradation by mixed-cultures of fungi and bacteria

Aromatic compounds and metalloid oxyanions are abundant in the environment due to natural resources and industrial wastes. The high toxicity of phenol and tellurite poses a significant threat to all forms of life. A halotolerant bacterium was isolated and identified as Lysinibacillus sp. EBL303. The remediation analysis shows that 500 mg/L phenol and 0.5 mM tellurite can be remediated entirely in separate cultures within 74 and 56 h, respectively. In addition, co-remediation of pollutants resulted in the same phenol degradation and 27% less tellurite reduction within 98 h. Since phenol and tellurite exhibited inhibitory behavior, their removal kinetics fitted well with the first-order model. In the characterization of biosynthesized tellurium nanoparticles (TeNPs), transmission electron microscopy, dynamic light scattering, FE-SEM, and dispersive X-ray (EDX) showed that the separated intracellular TeNPs were spherical and consisted of only tellurium with 22–148 nm in size. Additionally, investigations using X-ray diffraction and Fourier-transform infrared spectroscopy revealed proteins and lipids covering the surface of these amorphous TeNPs. Remarkably, this study is the first report to demonstrate the simultaneous bioremediation of phenol and tellurite and the biosynthesis of TeNPs, indicating the potential of Lysinibacillus sp. EBL303 in this matter, which can be applied to environmental remediation and the nanotechnology industry.

show abstract

“…Which t stands for time, Ct for concentration at time t, C0 for initial concentration, and K for removal rate constant 30 , 31 .…”

Section: Methodsmentioning

confidence: 99%

Simultaneous bioremediation of phenol and tellurite by Lysinibacillus sp. EBL303 and characterization of biosynthesized Te nanoparticles

Hosseini

Lashani

Moghimi

2023

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, rhamnolipid positively affected long-chain alkanes, HMW PAHs, and complex biomarkers degradation in the order of complex biomarkers > long-chain aliphatics > HMW PAHs 37 . Also, another study showed that both rhamnolipids and tween 80 performed better at increasing the degradation of tetracosane than pyrene 28 .…”

Section: Resultsmentioning

confidence: 98%

“…On the contrary, P. aeruginosa ATCC 10145 had more positive effects on tetracosane degradation and increased it from 71.3 to 83.7%. P. aeruginosa ATCC 10145 is a biosurfactant producer, so its contribution to the performance of mixed-culture is increasing the bioavailability of carbon sources, and some prior studies have noted that generally, surface-active compounds have a lesser effect on PAHs 28 , 37 . In a similar study, the effect of Rhamnolipid (same as P. aeruginosa ATCC 10145 biosurfactant) produced by Pseudomonas sp.…”

Section: Resultsmentioning

confidence: 99%

“…The consortium showed threefold higher pyrene degradation than individual Mycobacterium species 4 . Furthermore, in a study investigating pyrene and tetracosane removal by different fungal, bacterial, and fungal-bacterial mixed-cultures, the bacterial mixed-culture had the lowest capacity 28 . Most of the species in this bacterial consortium had similar roles and behaviors in pyrene and tetracosane degradation.…”

Section: Resultsmentioning

confidence: 99%

“…Pyrene, a 4-ring PAH, and tetracosane, a 24-carbon aliphatic, have often been used as model hydrocarbons due to their abundance and structural similarity with other HMW hydrocarbons 4 , 28 . This study tested the degradation efficiency of pyrene and tetracosane by a newly isolated B. subtilis at various salinities (0–10% w/v).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biodegradation of high molecular weight hydrocarbons under saline condition by halotolerant Bacillus subtilis and its mixed cultures with Pseudomonas species

Ghorbannezhad

Moghimi

Dastgheib³

2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

Biodegradation of high-molecular-weight petroleum hydrocarbons in saline conditions appears to be complicated and requires further investigation. This study used heavy crude oil to enrich petroleum-degrading bacteria from oil-contaminated saline soils. Strain HG 01, with 100% sequence similarity to Bacillussubtilis, grew at a wide range of salinities and degraded 55.5 and 77.2% of 500 mg/l pyrene and 500 mg/l tetracosane, respectively, at 5% w/v NaCl. Additionally, a mixed-culture of HG 01 with Pseudomonasputida and Pseudomonasaeruginosa, named TMC, increased the yield of pyrene, and tetracosane degradation by about 20%. Replacing minimal medium with treated seawater (C/N/P adjusted to 100/10/1) enabled TMC to degrade more than 99% of pyrene and tetracosane, but TMC had lesser degradation in untreated seawater than in minimal medium. Also, the degradation kinetics of pyrene and tetracosane were fitted to a first-order model. Compared to B.subtilis, TMC increased pyrene and tetracosane's removal rate constant (K1) from 0.063 and 0.110 per day to 0.123 and 0.246 per day. TMC also increased the maximum specific growth rate of B.subtilis, P.putida, and P.aeruginosa, respectively, 45% higher in pyrene, 24.5% in tetracosane, and 123.4% and 95.4% higher in pyrene and tetracosane.

show abstract

Molecular advances in mycoremediation of polycyclic aromatic hydrocarbons: Exploring fungal bacterial interactions

Bokade

Bajaj

2023

J Basic Microbiol

View full text Add to dashboard Cite

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous high global concern environmental pollutants and tend to bioaccumulate due to hydrophobic properties. These xenobiotics, having variable concentrations along different matrices, gradually undergo various physical, chemical, and biological transformation processes. Myco‐remediation aids accelerated degradation by effectively transforming complex ring structures to oxidized/hydroxylated intermediates, which can further funnel to bacterial degradation pathways. Exploitation of such complementing fungal−bacterial enzymatic activity can overcome certain limitations of incomplete bioremediation process. Furthermore, high‐throughput molecular methods can be employed to unveil community structure, taxon abundance, coexisting community interactions, and metabolic pathways under stressed conditions. The present review critically discusses the role of different fungal phyla in PAHs biotransformation and application of fungal−bacterial cocultures for enhanced mineralization. Moreover, recent advances in bioassays for PAH residue detection, monitoring, developing xenobiotics stress‐tolerant strains, and application of fungal catabolic enzymes are highlighted. Application of next‐generation sequencing methods to reveal complex ecological networks based on microbial community interactions and data analysis bias in performing such studies is further discussed in detail. Conclusively, the review underscores the application of mixed‐culture approach by critically highlighting in situ fungal−bacterial community nexus and its role in complete mineralization of PAHs for the management of contaminated sites.

show abstract

Evaluation of pyrene and tetracosane degradation by mixed-cultures of fungi and bacteria

Cited by 18 publications

References 42 publications

Simultaneous bioremediation of phenol and tellurite by Lysinibacillus sp. EBL303 and characterization of biosynthesized Te nanoparticles

Simultaneous bioremediation of phenol and tellurite by Lysinibacillus sp. EBL303 and characterization of biosynthesized Te nanoparticles

Biodegradation of high molecular weight hydrocarbons under saline condition by halotolerant Bacillus subtilis and its mixed cultures with Pseudomonas species

Molecular advances in mycoremediation of polycyclic aromatic hydrocarbons: Exploring fungal bacterial interactions

Contact Info

Product

Resources

About