Evaluation of quantitative polymerase chain reaction markers for the detection of breast cancer cells in ovarian tissue stored for fertility preservation

Bockstaele, Laurence; Boulenouar, Selma; Steen, Géraldine Van Den; Dechene, Julie; Tsépélidis, Sophie; Craciun, Ligia; Noël, Jean‐Christophe; Demeestere, Isabelle

doi:10.1016/j.fertnstert.2015.04.036

Cited by 11 publications

(4 citation statements)

References 34 publications

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“…The lack of specific immunological tools for species such as bovine can make the results of xenograft development difficult to interpret. The PCR method could be used to detect transplanted cells using DNA extracted from frozen tissue [8,9]. Here, we propose an alternative method to determine the species-specific mammary tissue that develops after xenotransplantation using genomic DNA extracted from fixed tissue.…”

Section: Methods Summarymentioning

confidence: 99%

Genomic DNA PCR Analysis to Assess Xenograft Development in Mouse Mammary Gland

et al. 2020

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The mouse transplantation model remains the most relevant methodology to assess the functional capacities of mammary cells and is particularly appropriate for investigations regarding mammary stem cells, whatever the species studied. Following xenotransplantation in mice mammary fat pad, the development of the xenograft is commonly evaluated by immunohistology. Here, we present a simple and rapid method to control the species specificity of a xenograft based on genomic DNA PCR amplification. DNA is extracted from the fixed samples intended for histology, thus allowing the reuse of precious samples. Standard and digital droplet PCR (requiring low DNA quantities) methods have been used to make the present method suitable for the analysis of xenotransplanted samples.

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Section: Methods Summarymentioning

confidence: 99%

Genomic DNA PCR Analysis to Assess Xenograft Development in Mouse Mammary Gland

et al. 2020

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“…In patients undergoing ovarian tissue cryopreservation an oophorectomy is performed soon after cancer diagnosis. In these patients, disseminated tumor cells may not yet have outgrown into overt metastases and may appear as micrometastases in the ovarian tissues [23, 26, 27]. The chance that tumor cells will then be overlooked is presumably greater.…”

Section: Discussionmentioning

confidence: 99%

Morphological and phenotypical features of ovarian metastases in breast cancer patients

et al. 2017

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BackgroundAutotransplantation of frozen-thawed ovarian tissue is a method to preserve ovarian function and fertility in patients undergoing gonadotoxic therapy. In oncology patients, the safety cannot yet be guaranteed, since current tumor detection methods can only exclude the presence of malignant cells in ovarian fragments that are not transplanted. We determined the need for a novel detection method by studying the distribution of tumor cells in ovaries from patients with breast cancer. Furthermore, we examined which cell-surface proteins are suitable as a target for non-invasive tumor-specific imaging of ovarian metastases from invasive breast cancer.MethodsUsing the nationwide database of the Dutch Pathology Registry (PALGA), we identified a cohort of 46 women with primary invasive breast cancer and ovarian metastases. The localization and morphology of ovarian metastases were determined on hematoxylin-and-eosin-stained sections. The following cell-surface markers were immunohistochemically analyzed: E-cadherin, epithelial membrane antigen (EMA), human epidermal growth receptor type 2 (Her2/neu), carcinoembryonic antigen (CEA), αvβ6 integrin and epithelial cell adhesion molecule (EpCAM).ResultsThe majority of ovarian metastases (71%) consisted of a solitary metastasis or multiple distinct nodules separated by uninvolved ovarian tissue, suggesting that ovarian metastases might be overlooked by the current detection approach. Combining the targets E-cadherin, EMA and Her2/neu resulted in nearly 100% detection of ductal ovarian metastases, whereas the combination of EMA, Her2/neu and EpCAM was most suitable to detect lobular ovarian metastases.ConclusionsExamination of the actual ovarian transplants is recommended. A combination of targets is most appropriate to detect ovarian metastases by tumor-specific imaging.Electronic supplementary materialThe online version of this article (doi:10.1186/s12885-017-3191-y) contains supplementary material, which is available to authorized users.

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“…Other studies assessed the usefulness of molecular markers for the detection of residual disease in the ovarian tissue of breast cancer patients. Using PCR technique, mammaglobin 1 (MGB1), gross cystic disease fluid protein 15, and small breast epithelial mucin showed the highest predictive values in the detection of micro-metastases in ovarian tissue of breast cancer [60][61][62][63] . Hoekman, et al detected positive MGB1 expression in ovarian tissue in one out of three patients with breast cancer and sentinel lymph node involvement 61 .…”

Section: Tamoxifen-associated Ovarian Hyperstimulationmentioning

confidence: 99%

Viable Options for Fertility Preservation in Breast Cancer Patients: A Focus on Latin America

Lambertini

Goldrat

Barragán-Carrillo

et al. 2017

RIC

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Thanks to the improved survival outcomes observed in recent years, a growing attention has been given to the quality of life issues faced by young women with breast cancer such as fertility preservation and concerns related to future pregnancies.However, several challenges remain for young women with breast cancer considering undergoing fertility preservation strategies.Further specific issues on this regard should be taken into account in Latin America, where patients and physicians face particular barriers that hinder the routine adoption of this practice. Hence, further efforts are needed to overcome these deficiencies and improve the correct referral of breast cancer patients to fertility preservation strategies.The aim of the present review is to focus on the risk of anticancer treatment-related premature ovarian failure and infertility in young breast cancer patients, to summarize the current knowledge on the available options for fertility preservation, and to discuss the safety issues of pregnancy in breast cancer survivors. Furthermore, this review aims to highlight the specific clinical challenges in this field encountered by healthcare providers and young breast cancer patients from Latin American countries. (REV INVES CLIN. 2017;69:103-13)

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Evaluation of quantitative polymerase chain reaction markers for the detection of breast cancer cells in ovarian tissue stored for fertility preservation

Cited by 11 publications

References 34 publications

Genomic DNA PCR Analysis to Assess Xenograft Development in Mouse Mammary Gland

Genomic DNA PCR Analysis to Assess Xenograft Development in Mouse Mammary Gland

Morphological and phenotypical features of ovarian metastases in breast cancer patients

Viable Options for Fertility Preservation in Breast Cancer Patients: A Focus on Latin America

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