Evaluation of radiation injury by <sup>1</sup>H and <sup>31</sup>P NMR of human urine

Yushmanov, Victor E.

doi:10.1002/mrm.1910310107

Cited by 8 publications

(4 citation statements)

References 19 publications

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“…Previous studies have used nuclear magnetic resonance [24], gas chromatography-mass spectrometry (GC-MS) [25], or liquid chromatography-mass spectrometry (LC-MS) [26] to measure metabolite levels after irradiation, and only a few employed capillary electrophoresis-mass spectrometry (CE-MS). These techniques have varying sensitivities, specificities, and detection limits.…”

Section: Introductionmentioning

confidence: 99%

Identification of Potential Biomarkers of Radiation Exposure in Blood Cells by Capillary Electrophoresis Time-of-Flight Mass Spectrometry

Sun

Inaba

Kanzaki

et al. 2020

IJMS

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Biodosimetry is a useful method for estimating personal exposure doses to ionizing radiation. Studies have identified metabolites in non-cellular biofluids that can be used as markers in biodosimetry. Levels of metabolites in blood cells may reflect health status or environmental stresses differentially. Here, we report changes in the levels of murine blood cell metabolites following exposure to X-rays in vivo. Levels of blood cell metabolites were measured by capillary electrophoresis time-of-flight mass spectrometry. The levels of 100 metabolites were altered substantially following exposure. We identified 2-aminobutyric acid, 2′-deoxycytidine, and choline as potentially useful markers of radiation exposure and established a potential prediction panel of the exposure dose using stepwise regression. Levels of blood cell metabolites may be useful biomarkers in estimating exposure doses during unexpected radiation incidents.

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Section: Introductionmentioning

confidence: 99%

Identification of Potential Biomarkers of Radiation Exposure in Blood Cells by Capillary Electrophoresis Time-of-Flight Mass Spectrometry

Sun

Inaba

Kanzaki

et al. 2020

IJMS

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“…One study, however, investigated possible radiation injury with no prior hypothesis except that signs of disturbances in cellular metabolism caused by radiation exposure after the Chernobyl reactor incident should be detectable in urine. 1 H and 31 P NMR analysis of human urine revealed some poorly defined changes in “ N -trimethyl groups” and in creatinine, citric acid, glycine and hippuric acid (24). …”

Section: Introductionmentioning

confidence: 99%

Radiation Metabolomics. 1. Identification of Minimally Invasive Urine Biomarkers for Gamma-Radiation Exposure in Mice

et al. 2008

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Gamma-radiation exposure has both short-and long-term adverse health effects. The threat of modern terrorism places human populations at risk for radiological exposures, yet current medical countermeasures to radiation exposure are limited. Here we describe metabolomics for γ-radiation biodosimetry in a mouse model. Mice were γ-irradiated at doses of 0, 3 and 8 Gy (2.57 Gy/min), and urine samples collected over the first 24 h after exposure were analyzed by ultra-performance liquid chromatography-time-of-flight mass spectrometry (UPLC-TOFMS). Multivariate data were analyzed by orthogonal partial least squares (OPLS). Both 3-and 8-Gy exposures yielded distinct urine metabolomic phenotypes. The top 22 ions for 3 and 8 Gy were analyzed further, including tandem mass spectrometric comparison with authentic standards, revealing that N-hexanoylglycine and β-thymidine are urinary biomarkers of exposure to 3 and 8 Gy, 3-hydroxy-2-methylbenzoic acid 3-O-sulfate is elevated in urine of mice exposed to 3 but not 8 Gy, and taurine is elevated after 8 but not 3 Gy. Gene Expression Dynamics Inspector (GEDI) self-organizing maps showed clear doseresponse relationships for subsets of the urine metabolome. This approach is useful for identifying mice exposed to γ radiation and for developing metabolomic strategies for noninvasive radiation biodosimetry in humans.

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“…Variations in the metabolite concentrations noted in survivors were considerable, and some distortions were seen as a result of water suppression and the use of deuterium. In addition, some metabolites such as methylene were pH sensitive, leading to misassignments of resonances [160]. Overall, NMR offers some advantages that include non-destructive sample analysis, high reproducibility, and the ability to perform accurate quantification [154,161].…”

Section: Metabolomic Biodosimetry Technologiesmentioning

confidence: 99%

Metabolomics in Radiation Biodosimetry: Current Approaches and Advances

et al. 2020

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Triage and medical intervention strategies for unanticipated exposure during a radiation incident benefit from the early, rapid and accurate assessment of dose level. Radiation exposure results in complex and persistent molecular and cellular responses that ultimately alter the levels of many biological markers, including the metabolomic phenotype. Metabolomics is an emerging field that promises the determination of radiation exposure by the qualitative and quantitative measurements of small molecules in a biological sample. This review highlights the current role of metabolomics in assessing radiation injury, as well as considerations for the diverse range of bioanalytical and sampling technologies that are being used to detect these changes. The authors also address the influence of the physiological status of an individual, the animal models studied, the technology and analysis employed in interrogating response to the radiation insult, and variables that factor into discovery and development of robust biomarker signatures. Furthermore, available databases for these studies have been reviewed, and existing regulatory guidance for metabolomics are discussed, with the ultimate goal of providing both context for this area of radiation research and the consideration of pathways for continued development.

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Evaluation of radiation injury by ¹H and ³¹P NMR of human urine

Cited by 8 publications

References 19 publications

Identification of Potential Biomarkers of Radiation Exposure in Blood Cells by Capillary Electrophoresis Time-of-Flight Mass Spectrometry

Identification of Potential Biomarkers of Radiation Exposure in Blood Cells by Capillary Electrophoresis Time-of-Flight Mass Spectrometry

Radiation Metabolomics. 1. Identification of Minimally Invasive Urine Biomarkers for Gamma-Radiation Exposure in Mice

Metabolomics in Radiation Biodosimetry: Current Approaches and Advances

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Evaluation of radiation injury by 1H and 31P NMR of human urine

Cited by 8 publications

References 19 publications

Identification of Potential Biomarkers of Radiation Exposure in Blood Cells by Capillary Electrophoresis Time-of-Flight Mass Spectrometry

Identification of Potential Biomarkers of Radiation Exposure in Blood Cells by Capillary Electrophoresis Time-of-Flight Mass Spectrometry

Radiation Metabolomics. 1. Identification of Minimally Invasive Urine Biomarkers for Gamma-Radiation Exposure in Mice

Metabolomics in Radiation Biodosimetry: Current Approaches and Advances

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Evaluation of radiation injury by ¹H and ³¹P NMR of human urine