Evaluation of radioprotective effect of carnosine (beta- alanyl-1- histidine) on the wound healing in rats

Abstract

he purpose of this study was to evaluate the radioprotective effect of carnosine (β-alanyl-1-histidine) on the wound healing in rats. Therefore, 48 male rats were submitted to a surgical procedure to perform a rectangular wound in the anteriordorsal region. The animals were divided into 4 experimental groups randomly chosen: control; irradiated; carnosine irradiated and carnosine group. The irradiated and carnosine irradiated group were exposed to a dose (6Gy) of gamma irradiation, in the whole body, 72 hours after surgery. The carnosine and carnosine irradiated groups, in addition to the surgical procedure and the irradiation, received two doses of carnosine aqueous solution, the first one being injected 48 hours after surgery, and the second one 1 hour and 30 minutes before irradiation. The tissue repair of the 4 groups was evaluated at 4, 7, 14, and 21 days after inflicting the wound, by morphological, histochemical and histophysical methods. At all examined periods, it could be observed that the animals from the carnosine irradiated group presented a better developed granulation tissue than the irradiated group and closely similar to that of the control group. Thus, under the experimental conditions used, it was possible to conclude that carnosine is an effective radioprotective substance. Uniterms: Radiation, ionizing; Radiation-protective agents; Carnosine; Wound healing. objetivo deste estudo foi avaliar o efeito radioprotetor da carnosina (β-alanil-1-histidina) no processo de cicatrização em ratos. Para isto, 48 ratos machos foram submetidos a um procedimento cirúrgico para realização de uma ferida retangular na região dorsal anterior. Os animais foram divididos aleatoriamente em 4 grupos experimentais: controle, irradiado, carnosina irradiado e carnosina. Os grupos carnosina e carnosina irradiado foram exposto a uma dose de corpo todo de 6 Gy de radiação gama, 72 horas após a cirurgia para confecção da ferida. O grupo carnosina e carnosina irradiado, adicionalmente, ao procedimento cirúrgico e a irradiação, receberam duas doses de solução aquosa de carnosina, a primeira sendo injetada 48 horas após a cirurgia, e a segunda 1 hora e 30 minutos antes da irradiação. A reparação tecidual nos 4 grupos foi avaliada 4, 7, 14 e 21 dias após a realização da ferida, por métodos morfológicos, histoquímicos e histofísicos. Em todos os períodos examinados, pode ser observado que os animais do grupo carnosina irradiado apresentaram um melhor desenvolvimento do tecido de granulação do que o grupo irradiado e muito similar ao do grupo controle. Logo, de acordo com as condições experimentais usadas, foi possível concluir que a carnosina é uma substância radioprotetora efetiva. Unitermos: Radiação ionizante; Protetores contra radiação; Carnosina; Cicatrização de feridas. 2005; 13(3): 253-8 J Appl Oral Sci

Gut Microbiota-Derived l-Histidine/Imidazole Propionate Axis Fights against the Radiation-Induced Cardiopulmonary Injury

“…l -Histidine was employed as an important component of preparation for organ preservation and myocardial protection in cardiac surgery [ 33 ]. In addition, studies have reported that carnosine (beta-alanyl-1-histidine) represents radioprotective effects on wound healing in rats [ 34 ] and can reduce radiation-induced lung damage [ 35 ]. In the present study, we screened out a single l -Histidine from intestinal microorganism-produced metabolites and found that oral gavage of l -Histidine educated and tuned the gut bacteria taxonomic proportions of local chest-irradiated mice.…”

Carnosine and Related Peptides: Therapeutic Potential in Age-Related Disorders

“…Carnosine is the most studied compound in the group of imidazolic dipeptides and presents putative antioxidant [20], bivalent metal ion chelating [21], radioprotective [22], muscular proton buffering [23], and reactive carbonyl scavenger activities [24]. The latter property was also suggested to the D-enantiomer of carnosine [25].…”