Evaluation of Raftlin Mrna in Hepatocellular Carcinoma

Boshra, Mariam Sameh; Meteini, Mahmoud Shawky El; Shafei, Ayman; Matboly, Marwa; Ali, H.A.M.

doi:10.21608/asmj.2019.111197

Cited by 2 publications

(1 citation statement)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ephrin/Eph signaling plays pivotal roles in various developmental contexts such as axon guidance, tissue boundary formation, and angiogenesis [ 60 ], but its dysregulation is frequently observed in various types of cancers [ 61 ]. Similarly, raftlin , a persistent upregulated gene in this study, tends to be upregulated in patients of HCC compared to healthy controls [ 62 ]. Raftlin is one of the major lipid raft proteins and is known to function in adaptive immunity by mediating B-cell receptor (BCR) and T-cell receptor (TCR) signaling in lymphocytes, as well as in innate immunity by mediating uptake of ligand-bound Toll-like receptors (TLR) 3/4 via endocytosis in dendritic cells and macrophages [ 63 , 64 ].…”

Section: Discussionmentioning

confidence: 79%

High-fat diet in early life triggers both reversible and persistent epigenetic changes in the medaka fish (Oryzias latipes)

Inoue,

Suzuki,

Kunishima

et al. 2023

BMC Genomics

View full text Add to dashboard Cite

Background The nutritional status during early life can have enduring effects on an animal’s metabolism, although the mechanisms underlying these long-term effects are still unclear. Epigenetic modifications are considered a prime candidate mechanism for encoding early-life nutritional memories during this critical developmental period. However, the extent to which these epigenetic changes occur and persist over time remains uncertain, in part due to challenges associated with directly stimulating the fetus with specific nutrients in viviparous mammalian systems. Results In this study, we used medaka as an oviparous vertebrate model to establish an early-life high-fat diet (HFD) model. Larvae were fed with HFD from the hatching stages (one week after fertilization) for six weeks, followed by normal chow (NC) for eight weeks until the adult stage. We examined the changes in the transcriptomic and epigenetic state of the liver over this period. We found that HFD induces simple liver steatosis, accompanied by drastic changes in the hepatic transcriptome, chromatin accessibility, and histone modifications, especially in metabolic genes. These changes were largely reversed after the long-term NC, demonstrating the high plasticity of the epigenetic state in hepatocytes. However, we found a certain number of genomic loci showing non-reversible epigenetic changes, especially around genes related to cell signaling, liver fibrosis, and hepatocellular carcinoma, implying persistent changes in the cellular state of the liver triggered by early-life HFD feeding. Conclusion In summary, our data show that early-life HFD feeding triggers both reversible and persistent epigenetic changes in medaka hepatocytes. Our data provide novel insights into the epigenetic mechanism of nutritional programming and a comprehensive atlas of the long-term epigenetic state in an early-life HFD model of non-mammalian vertebrates.

show abstract

Section: Discussionmentioning

confidence: 79%

High-fat diet in early life triggers both reversible and persistent epigenetic changes in the medaka fish (Oryzias latipes)

Inoue,

Suzuki,

Kunishima

et al. 2023

BMC Genomics

View full text Add to dashboard Cite

show abstract

High-fat diet in early life triggers both reversible and persistent epigenetic changes in the medaka fish (Oryzias latipes)

Inoue

Suzuki

Kunishima

et al. 2023

Preprint

View full text Add to dashboard Cite

The nutritional status during early life can have enduring effects on an animal's metabolism, although the mechanisms underlying these long-term effects are still unclear. Epigenetic modifications are considered a prime candidate mechanism for encoding early-life nutritional memories during this critical developmental period. However, the extent to which these epigenetic changes occur and persist over time remains uncertain, in part due to challenges associated with directly stimulating the fetus with specific nutrients in viviparous mammalian systems. In this study, we used medaka as an oviparous vertebrate model to establish an early-life high-fat diet (HFD) model. Larvae were fed with HFD from the hatching stages (one week after fertilization) for six weeks, followed by normal chow (NC) for eight weeks until the adult stage. We examined the changes in the transcriptomic and epigenetic state of the liver over this period. We found that HFD induces fatty liver phenotypes, accompanied by drastic changes in the hepatic transcriptome, chromatin accessibility, and histone modifications, especially in metabolic genes. These changes were largely reversed after the long-term NC, demonstrating the high plasticity of the epigenetic state in hepatocytes. However, we found a certain number of genomic loci showing non-reversible epigenetic changes, especially around genes related to cell signaling, implying persistent changes in the cellular state of the liver triggered by early-life HFD feeding. Our data provide novel insights into the epigenetic mechanism of nutritional programming and a comprehensive atlas of the long-term epigenetic state in an early-life HFD model of non-mammalian vertebrates.

show abstract

Evaluation of Raftlin Mrna in Hepatocellular Carcinoma

Cited by 2 publications

References 5 publications

High-fat diet in early life triggers both reversible and persistent epigenetic changes in the medaka fish (Oryzias latipes)

High-fat diet in early life triggers both reversible and persistent epigenetic changes in the medaka fish (Oryzias latipes)

High-fat diet in early life triggers both reversible and persistent epigenetic changes in the medaka fish (Oryzias latipes)

Contact Info

Product

Resources

About