Evaluation of Real Time PCR, ELISA and Direct Strip Tests for Detection of Helicobacter pylori Infection in Patients with Gastrointestinal Illnesses

Erzooki, Asmaa; Manhal, Falah Salim

doi:10.9734/bjmmr/2016/25631

Cited by 2 publications

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“…To avoid the harsh condition in the gastric lumen, this bacteria have developed an antibiotic-resistant to the stomach acid of the microbial through colonization in a very narrow place of gastric lactation and secretion of the urease which analyses urea located in the medium to ammonia which have the effect of the acidic acid around in the stomach lining which enables them to stay in the human stomach lifelong if not treated with antibiotics [6]. Therefore, this bacterium causes many diseases such as chronic gastritis, gastric ulcers, duodenal ulcers, Gastric cancer and Mucosa-associated lymphoid-tissue lymphoma [7]. The transmission of H. pylori may occur via oraloral, fecal-oral, gastric-oral or iatrogenic routes [8].…”

Section: Introductionmentioning

confidence: 99%

The prevalence of Helicobacter pylori infection in Baqubah city

Jameel¹,

Motib²,

Athab³

2020

DJM

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Background: Helicobacter pylori are the most common cause's peptic ulcer disease, and it is transmitted by oral-oral, fecal-oral and gastric oral. The prevalence of this bacteria depends on age, sex, smoking or non-smoking and also chronic disease such as diabetes. Objective: To determine the infection of H. pylori in Baqubah city and its associated with age and gender of patients. Patients and Methods: H. pylori diagnosed by used rapid urease test and histopathology test. From 200 studied groups, 127 (63.5%) males and 73 (36.5%) females ages ranged from (10 to ≥ 60) years were collected from Baqubah teaching hospitals, during the period from September 2018 till January 2019. From 200 studied groups 110 considered patients after initial diagnosis by endoscopic unit (presence symptoms) and 90 considered control group (absence symptoms). Two types of samples were collected from each studied groups including gastric biopsy specimens for rapid urease test and histopathology test to detect H. pylori infection. Results:The result of this study shows that H. pylori infection was detected by rapid urease test which appears positive results in 86 patients (78.2 %), and the positive results of histopathology test were 10 (9.0%). The incidence of H. pylori infection in male more than in female as the percentage (70.00%) and (30.00%), respectively. Minimum age was 10 years and maximum was ≥ 60. The highest age specific frequency in studied groups is in the age group (40-49) & (50-59) years old. Conclusion:The prevalence rate of gastric ulcer caused by H. pylori in Baqubah city was high and it is detected by rapid urease test which showed more accurate than histopathological test. In addition, the results showed that the rate of incidence in males more than in females, and in (40-49)&(50-59) more than other ages.

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Section: Introductionmentioning

confidence: 99%

The prevalence of Helicobacter pylori infection in Baqubah city

Jameel¹,

Motib²,

Athab³

2020

DJM

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“…Helicobacter pylori is a gram negative and spiral-shaped bacterium which colonizes the human stomach mucoid lining (1) and linked with increased risk for numerous disease "chronic gastritis(GS), duodenal ulcer (DU), gastric ulcer (GU), dysplasia, mucosalassociated lymphoid tissue (MALT) lymphoma and gastric cancer (GC)" (2) .…”

Section: Introductionmentioning

confidence: 99%

Detection of, cagA and vacA Helicobacter pylori Virulence Genes in Gastric Biopsies of patients with Gastroduodenal disease using Polymerase chain reaction (PCR) technique

Al-Mahdawi¹,

Kareem²,

Hamdi³

2017

djps

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The objective of current study was to detect the prevalence of Helicobacter pylori by identifying 16SrDNA and to determine the virulence genes (ureA, cagA and vacA) in biopsy specimens from patients suffering gastroduodenal disease using polymerase chain reaction (PCR). Forty samples were obtained by gastroenterologists during endoscopy from gastric antral of suspected individual attending endoscopy unit at Baqubah Teaching Hospital, Diyala, Iraq, during the period from September 2015 to February 2016. According to the endoscopic finding the patients were allocated into four groups of gastroduodenal diseases and control, which include gastritis (GS), duodenal ulcer (DU), gastric ulcer (GU), gastric cancer (GC), their rates were 30% (12), 20% (8), 17.5% (7), 7.5% (3) and 25% (10), respectively. DNA was extracted from the biopsies and subsequently used for PCR detection of H. pylori and the virulence genes using specific primers. The results shows that 60% of samples were positive for H. pylori, of these positive samples, 91,66%, 66.66%, and 48.83%, were shown to have the virulence genes, ureA, cagA, and vacA, respectively. It is important to mention that cagA shown the highest prevalence rate in gastric cancer cases in comparison with vacA gene. further studies are required to study the link between cagA gene and

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Evaluation of Real Time PCR, ELISA and Direct Strip Tests for Detection of Helicobacter pylori Infection in Patients with Gastrointestinal Illnesses

Cited by 2 publications

References 0 publications

The prevalence of Helicobacter pylori infection in Baqubah city

The prevalence of Helicobacter pylori infection in Baqubah city

Detection of, cagA and vacA Helicobacter pylori Virulence Genes in Gastric Biopsies of patients with Gastroduodenal disease using Polymerase chain reaction (PCR) technique

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