Evaluation of RECK mRNA and RECK protein in tissue and serum of breast cancer patients

AbouGhalia, Azza H; Khairy, Eman; Fouad, Manar Ahmed; Boshnak, Hussein

doi:10.1016/j.genrep.2018.05.005

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…RECK is a new tumor suppressor gene first discovered by Professor Takahashi. It can inhibit tumor invasion and metastasis by downregulating the expression of MMP-9, and widely expressed in human blood, various solid tissues, and undifferentiated cells [12,18]. Studies have found the significantly reduced expression of RECK in a variety of tumors, including breast cancer, colorectal cancer, cholangiocarcinoma, lung cancer, cervical cancer, pancreatic cancer, hepatocellular carcinoma, and gastric cancer, etc.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have found the significantly reduced expression of RECK in a variety of tumors, including breast cancer, colorectal cancer, cholangiocarcinoma, lung cancer, cervical cancer, pancreatic cancer, hepatocellular carcinoma, and gastric cancer, etc. [18][19][20][21][22][23][24][25], and it was mostly associated with the prognosis of tumors [15,23,26]. RECK also has been demonstrated to be involved in the development of many nonmalignant diseases, including myocardial fibrosis, coronary atherosclerosis, asthma, etc.…”

Section: Discussionmentioning

confidence: 99%

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Association between the Reduced Expression of RECK and Neutrophilic Inflammation in Chronic Obstructive Pulmonary Disease

Wang,

Su,

Liu

et al. 2024

Int Arch Allergy Immunol

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Introduction: Reversion-inducing cysteine-rich protein with Kazal motifs (RECK), a recently discovered inhibitor of matrix metalloproteinase (MMP). There is a large number of chronic obstructive pulmonary disease (COPD) patients worldwide; however, the role of RECK on COPD has not been studied. This study explored the expression of RECK in COPD patients and its effect on neutrophil function to provide a new scientific basis for the prevention and treatment of COPD. Method: Fifty patients with acute exacerbation of COPD and fifty healthy controls were enrolled in the study. RECK was detected in lung tissue, sputum, and plasma of subjects as well as in BEAS-2B cells stimulated with cigarette smoke extract (CSE) by immunohistochemistry, ELISA, and qRT-PCR. Meanwhile, lung function (FEV1%pred) and inflammatory cytokines (IL-6 and IL-8) were examined, and correlation analysis was performed with RECK expression. The effect of RECK on proliferation, apoptosis, migration, and inflammatory cytokines and its potential mechanism was further quantified by neutrophil stimulated with recombinant human RECK protein (rhRECK) combined with CSE using CCK8, flow cytometry, Transwell assay, qRT-PCR, ELISA, and Western analysis. Results: RECK was mainly expressed on airway epithelial cells in normal lung tissue and was significantly diminished in COPD patients. The levels of RECK in sputum and plasma were also significantly decreased in COPD patients. Pearson correlation analysis showed that RECK level in plasma was positively correlated with FEV1%pred (r = 0.458, p < 0.001) and negatively correlated with IL-6 and IL-8 (r = −0.386, −0.437; p = 0.006, 0.002) in COPD patients. The expression of RECK was decreased in BEAS-2B stimulated with CSE. The migration, inflammation, and MMP-9 expression of neutrophils were promoted by CSE, while inhibited by rhRECK. Conclusion: RECK is low expressed in COPD patients and negatively correlated with inflammation. It may inhibit the inflammation and migration of neutrophils by downregulating MMP-9.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Association between the Reduced Expression of RECK and Neutrophilic Inflammation in Chronic Obstructive Pulmonary Disease

Wang,

Su,

Liu

et al. 2024

Int Arch Allergy Immunol

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WITHDRAWN: Association between the reduced expression of RECK and neutrophilic inflammation in chronic obstructive pulmonary disease

Fang

Liu

et al. 2022

Preprint

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Objective Reversion-inducing cysteine-rich protein with Kazal motifs (RECK), a new inhibitor of matrix metalloproteinase (MMP), plays a vital role in tumor invasion. Patients with chronic obstructive pulmonary disease (COPD) are at higher risk of developing lung cancer, however, the role of RECK on COPD has not been studied. This study explored the expression of RECK in COPD patients and its effect on neutrophil function to provide a new scientific basis for the prevention and treatment of COPD. Method Fifty patients with acute exacerbation of COPD and fifty healthy controls were enrolled in the study. RECK was detected in lung tissue, sputum and plasma of subjects as well as in BEAS-2B cells stimulated with cigarette smoke extract (CSE) by immunohistochemistry, ELISA and qRT-PCR. Meanwhile, lung function (FEV1%pred) and inflammatory cytokines (IL-6 and IL-8) were examined, and correlation analysis was performed with RECK expression. The effect of RECK on proliferation, apoptosis, migration and inflammatory cytokines and its potential mechanism were further quantified by neutrophil stimulated with recombinant human RECK protein (rhRECK) combined with CSE using CCK8, flow cytometry, Transwell assay, qRT-PCR, ELISA and Western blot. Results RECK was mainly expressed on airway epithelial cells in normal lung tissue and was significantly diminished in COPD patients. The levels of RECK in sputum and plasma were also significantly decreased in COPD patients. Pearson correlation analysis showed that RECK level in plasma was positively correlated with FEV1%pred (r = 0.458, P < 0.001) and negatively correlated with IL-6 and IL-8 (r=-0.386, -0.437; P = 0.006, 0.002) in COPD patients. The expression of RECK was decreased in BEAS-2B stimulated with CSE. The migration, inflammation, and MMP-9 expression of neutrophils were promoted by CSE, while inhibited by rhRECK. Conclusions RECK is low expressed in COPD patients and negatively correlated with inflammation. It may inhibit the inflammation and migration of neutrophils by downregulating MMP-9.

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WITHDRAWN: Association between the reduced expression of RECK and neutrophilic inflammation in chronic obstructive pulmonary disease

2023

Preprint

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Evaluation of RECK mRNA and RECK protein in tissue and serum of breast cancer patients

Cited by 3 publications

References 22 publications

Association between the Reduced Expression of RECK and Neutrophilic Inflammation in Chronic Obstructive Pulmonary Disease

Association between the Reduced Expression of RECK and Neutrophilic Inflammation in Chronic Obstructive Pulmonary Disease

WITHDRAWN: Association between the reduced expression of RECK and neutrophilic inflammation in chronic obstructive pulmonary disease

WITHDRAWN: Association between the reduced expression of RECK and neutrophilic inflammation in chronic obstructive pulmonary disease

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