Evaluation of Recombinant Green Fluorescent Protein, Under Various Culture Conditions and Purification with HiTrap Hydrophobic Interaction Chromatography Resins

Penna, Thereza Christina Vessoni; Ishii, Marina; Pessoa, Adalberto; Nascimento, Laura Oliveira de; Souza, Luciana Cambricoli de; Cholewa, Olívia

doi:10.1007/978-1-59259-837-3_39

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Hydrophobic Interaction Chromatography1

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2005

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“…Poppenborg et al [25] optimized immobilized metal affinity separation of a histidine-rich protein tagged with GFP by tracking the fluorescence of the fusion protein. Since GFP is a highly hydrophobic protein, recovery of GFP-fusion proteins can be facilitated by using hydrophobic interaction chromatography (HIC) [26]. GFP has also been engineered to allow affinity purification.…”

Section: Reviewmentioning

confidence: 99%

Fluorescent proteins as tools to aid protein production

2005

Microb Cell Fact

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Fluorescent proteins are genetically encoded, highly versatile reporters useful for monitoring various aspects of recombinant protein production. In addition to the widely popular green fluorescent protein (GFP) from Aequorea victoria, a variety of other fluorescent proteins have been discovered that display a wide range of spectral properties. Synthetic variants have also been developed to overcome limitations associated with their wild-type counterparts. Having a large repertoire of fluorescent proteins with diverse traits opens new opportunities for rapid monitoring and optimization of recombinant protein production.

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Section: Reviewmentioning

confidence: 99%