Evaluation of recombinant LigB antigen-based indirect ELISA and latex agglutination test for the serodiagnosis of bovine leptospirosis in India

Deneke, Yosef; Thankappan, Sabarinath; Gogia, Neha; Lalsiamthara, Jonathan; Viswas, K.N.; Chaudhuri, Pallab

doi:10.1016/j.mcp.2014.01.001

Cited by 22 publications

(26 citation statements)

References 27 publications

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“…This could be explained on the basis that all those sera turned out to be MAT negative may not actually be negative as only 6 Leptospira serovars were used as antigens for antibody detection in MAT. However, the findings supported the prevailing view that the ELISA was more sensitive than MAT (Rana et al, 2012;Deneke et al, 2014).…”

Section: Sensitivity and Specificity Of Indirect Elisa And Matsupporting

confidence: 83%

Antioxidant Enzymes in Leaves of Susceptible and Resistant Okra Genotypes against YVMV

Patel¹,

Japda²,

Dhruve³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Section: Sensitivity and Specificity Of Indirect Elisa And Matsupporting

confidence: 83%

Antioxidant Enzymes in Leaves of Susceptible and Resistant Okra Genotypes against YVMV

Patel¹,

Japda²,

Dhruve³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…Traditionally, immunoagglutination is only used in a qualitative test; however, together with the narrow beam technique, immunoagglutination offers a simple, generally applicable, and non-hazardous method for fast and bacterium-specific quantitative detection [33,34,35,36,37,38,39,40,41,42,43,44,45,46]. More importantly, the agglutination reaction is a one-step reaction method mediated by specific reactions between antibodies immobilized on microbeads and antigens in the sample, requiring no further washing steps prior to detection.…”

Section: Resultsmentioning

confidence: 99%

Rapid Waterborne Pathogen Detection with Mobile Electronics

Wu¹,

Chen²,

Wang³

et al. 2017

Sensors

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Pathogen detection in water samples, without complex and time consuming procedures such as fluorescent-labeling or culture-based incubation, is essential to public safety. We propose an immunoagglutination-based protocol together with the microfluidic device to quantify pathogen levels directly from water samples. Utilizing ubiquitous complementary metal–oxide–semiconductor (CMOS) imagers from mobile electronics, a low-cost and one-step reaction detection protocol is developed to enable field detection for waterborne pathogens. 10 mL of pathogen-containing water samples was processed using the developed protocol including filtration enrichment, immune-reaction detection and imaging processing. The limit of detection of 10 E. coli O157:H7 cells/10 mL has been demonstrated within 10 min of turnaround time. The protocol can readily be integrated into a mobile electronics such as smartphones for rapid and reproducible field detection of waterborne pathogens.

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“…The main bottleneck involved in whole cell antigen based immunoassays for M. leachii is the preparation of whole cell antigen of M. leachii which is time-consuming and cumbersome. The advent of recombinant DNA technology made possible the generation of large quantities of purified recombinant proteins in heterologous hosts such as E. cloni for specific diagnosis of infectious diseases [ 28 ]. Moreover, recombinant protein based serological tests show high sensitivity and specificity, owing to high concentration of immunoreactive antigens and the lack of non-specific moieties present in whole cell preparations [ 29 ].…”

Section: Resultsmentioning

confidence: 99%

Cloning and expression of P67 protein of Mycoplasma leachii

et al. 2017

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Aim:The present study was undertaken to clone, express and study the immunogenicity of P67 protein of Mycoplasma leachii.Materials and Methods:P67 gene was amplified from genomic DNA of M. leachii. The polymerase chain reaction (PCR) product was inserted in pRham N-His SUMO Kan vector and was used to transform competent Escherichia cloni 10G cells. Recombinant protein expression was done by inducing cells with 0.2% Rhamnose. Purification was done using nickel nitrilotriacetic acid affinity chromatography. Western blot and dot blot analysis were performed to assess the immunoreactivity of P67 protein.Results:PCR amplicon size of P67 gene was found to be 1500 base pair. The size of the fusion protein with SUMO tag was 79 kDa in sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis. The recombinant P67 fusion protein expressed in pRham N-His SUMO Kan vector was found to be immunogenic in both western blot and dot blot analysis.Conclusion:Western blot and dot blot analysis of P67 protein of M. leachii revealed that the protein is immunogenic. Further work is needed to evaluate the role of P67 antigen of M. leachii as an immunodiagnostic agent.

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Evaluation of recombinant LigB antigen-based indirect ELISA and latex agglutination test for the serodiagnosis of bovine leptospirosis in India

Cited by 22 publications

References 27 publications

Antioxidant Enzymes in Leaves of Susceptible and Resistant Okra Genotypes against YVMV

Antioxidant Enzymes in Leaves of Susceptible and Resistant Okra Genotypes against YVMV

Rapid Waterborne Pathogen Detection with Mobile Electronics

Cloning and expression of P67 protein of Mycoplasma leachii

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