Evaluation of recombinant sporozoite antigen SPAG‐1 as a vaccine candidate against <i>Theileria annulata</i> by the use of different delivery systems

Boulter, Nicola R.; Brown, D. H.; Wilkie, Gwen; Williamson, Susanna; Kirvar, E.; Knight, Pamela; Glass, Elizabeth J.; Campbell, John; Morzaria, S.P.; Nene, Vishvanath; Musoke, A.J.; d’Oliveira, Christine; Gubbels, Marc‐Jan; Jongejan, Frans; Hall, Roger

doi:10.1046/j.1365-3156.1999.00453.x

Cited by 21 publications

(13 citation statements)

References 16 publications

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“…The chimeric particles not only induced high titers of neutralizing antibodies, and a significant T cell response, but also showed some evidence of protection against sporozoite challenge [131], which allowed to recommend them for inclusion into future multicomponent vaccine [132].…”

Section: Internal Insertionsmentioning

confidence: 95%

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HBV Core Particles as a Carrier for B Cell/T Cell Epitopes

Pumpens¹,

Grens²

2001

Intervirology

249

195

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In the middle 80s, recombinant hepatitis B virus cores (HBc) gave onset to icosahedral virus-like particles (VLPs) as a basic class of non-infectious carriers of foreign immunological epitopes. The recombinant HBc particles were used to display immunodominant epitopes of hepatitis B, C, and E virus, human rhinovirus, papillomavirus, hantavirus, and influenza virus, human and simian immunodeficiency virus, bovine and feline leukemia virus, foot-and-mouth disease virus, murine cytomegalovirus and poliovirus, and other virus proteins, as well as of some bacterial and protozoan protein epitopes. Practical applicability of the HBc particles as carriers was enabled by their ability to high level synthesis and correct self-assembly in heterologous expression systems. The interest in the HBc VLPs was reinforced by the resolution of their fine structure by electron cryomicroscopy and X-ray crystallography, which revealed an unusual α-helical organization of dimeric units of HBc shells, alternative packing into icosahedrons with T = 3 and T = 4 symmetry, and the existence of long protruding spikes. The tips of the latter seem to be the optimal targets for the display of foreign sequences up to 238 amino acid residues in length. Combination of numerous experimental data on epitope display with the precise structural information enables a knowledge-based design of diagnostic, and vaccine and gene therapy tools on the basis of the HBc particles.

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Section: Internal Insertionsmentioning

confidence: 95%

“…The chimeric HBc-SR1 particles, carrying a segment of a SPAG-1 of T. annulata is also regarded as a potential sporozoite vaccine challenge [131,132].…”

Section: Application Of Chimeric Hbc Particles As Vaccine Candidatesmentioning

confidence: 99%

HBV Core Particles as a Carrier for B Cell/T Cell Epitopes

Pumpens¹,

Grens²

2001

Intervirology

249

195

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“…Like the circumsporozoite protein (CSP) of Plasmodium sporozoites, the p67/SPAG1 proteins play a role in host cell recognition and entry, but they are not expressed in the schizont stage. Recombinant p67 and SPAG1 incorporated in adjuvant induce sporozoite neutralizing activity in cattle and induce immunity to sporozoite needle challenge in a proportion of immunized cattle. In most of these experiments, the sporozoite challenge dose was titrated to provide less than an LD 100 .…”

Section: Approaches To Development Of Subunit Vaccinesmentioning

confidence: 99%

“…It is likely that in the first of these categories of animals, no infection occurred, as these cattle were negative by PCR. Immunity induced by SPAG1 and different forms of SPAG1 has been less well studied, but as with p67 has been found to induce significant levels of immunity (~50%) to challenge . Interestingly, the SPAG1 protein has been shown to synergize with the protective efficacy of the Tams‐1 protein and a live attenuated T. annulata cell line, indicating that there is merit in assessing the role of multiple parasite antigens in increasing the efficacy of candidate vaccine antigens.…”

Section: Approaches To Development Of Subunit Vaccinesmentioning

confidence: 99%

Approaches to vaccination against Theileria parva and Theileria annulata

Nene

Morrison

2016

Parasite Immunology

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SummaryDespite having different cell tropism, the pathogenesis and immunobiology of the diseases caused by Theileria parva and Theileria annulata are remarkably similar. Live vaccines have been available for both parasites for over 40 years, but although they provide strong protection, practical disadvantages have limited their widespread application. Efforts to develop alternative vaccines using defined parasite antigens have focused on the sporozoite and intracellular schizont stages of the parasites. Experimental vaccination studies using viral vectors expressing T. parva schizont antigens and T. parva and T. annulata sporozoite antigens incorporated in adjuvant have, in each case, demonstrated protection against parasite challenge in a proportion of vaccinated animals. Current work is investigating alternative antigen delivery systems in an attempt to improve the levels of protection. The genome architecture and protein‐coding capacity of T. parva and T. annulata are remarkably similar. The major sporozoite surface antigen in both species and most of the schizont antigens are encoded by orthologous genes. The former have been shown to induce species cross‐reactive neutralizing antibodies, and comparison of the schizont antigen orthologues has demonstrated that some of them display high levels of sequence conservation. Hence, advances in development of subunit vaccines against one parasite species are likely to be readily applicable to the other.

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“…Recombinant forms of the antigen have been tested in vivo and while they have been found to attenuate the effects of a virulent challenge, the vaccine preparations have not afforded good protection (Boulter et al, 1999).…”

Section: Theileria Sporozoite Antigensmentioning

confidence: 99%