Evaluation of reference samples for the detection of Salmonella

Beckers, H. J.; Leusden, F. M. Van; Roberts, Diane; Pietzsch, O.; Price, T. H.; Schothorst, M. van; Beumer, R.R.; Peters, Richard; Kampelmacher, E. H.

doi:10.1016/0168-1605(86)90005-x

Cited by 13 publications

(5 citation statements)

References 8 publications

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“…This confirms the work of Beckers et al (1984) who clearly demonstrated that the concentration of salmonellas in reference samples of artificially contaminated milk powder decreased rapidly with increasing temperature. The results suggest that the extent of decrease depended on the temperature of storage; the higher the room temperature the greater the decrease.…”

Section: Discussionsupporting

confidence: 91%

“…The results suggest that the extent of decrease depended on the temperature of storage; the higher the room temperature the greater the decrease. This confirms the work of Beckers et al (1984) who clearly demonstrated that the concentration of salmonellas in reference samples of artificially contaminated milk powder decreased rapidly with increasing temperature. Such reference samples of milk powder should therefore be stored at 4°C.…”

Section: Discussionsupporting

confidence: 91%

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Collaborative study on the isolation of salmonella from artificially contaminated milk powder

Beckers

Leusden

Roberts³

et al. 1985

Journal of Applied Bacteriology

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The suitability of artificially contaminated milk powder as a substrate for salmonella reference samples and its stability under different storage conditions were studied. The need for a reconstitution step in the standard isolation method for salmonellas from milk powders was also investigated. When milk powder was examined in this way with a reconstitution step, differences in laboratory methods and/or storage times had no significant effect on the results after storage at 4 degrees C. With powder stored at room temperature there was a systematic decrease in the number of samples positive as the storage time increased. It is concluded therefore that milk powder contaminated with salmonellas should be stored at 4 degrees C. Examination of such milk powder with a reconstitution step yielded better results than without it and this step is therefore necessary for improving the reproducibility of the method. No significant differences were encountered between the standard isolation method and that used in the authors' laboratories. The results of this study indicate that milk powder is suitable as basic material for reference samples and that a reconstitution step should be included in the standard salmonella isolation procedure.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Collaborative study on the isolation of salmonella from artificially contaminated milk powder

Beckers

Leusden

Roberts³

et al. 1985

Journal of Applied Bacteriology

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“…Several authors (3,12,14,20,24) observed that RV is the most effective enrichment broth for Salmonella. However, in our study the difference between RV and TT was not significant.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry

Rall¹,

Rall²,

Aragon³

et al. 2005

Braz. J. Microbiol.

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We evaluated the effectiveness of Selenite Cystine (SC), Tetrathionate Brilliant Green (TBG) and Rappaport Vassiliadis (RV) broths for Salmonella isolation. We also tested three classic plating media, SalmonellaShigella Agar (SS), Brilliant Green Agar (BGA), Xylose Lysine Desoxycholate Agar (XLD) and two chromogenic agars, Rambach (RA) and CHROMagar Salmonella (CAS). Among 100 poultry carcasses, 29 were positive for Salmonella using all plating media combined. RV broth (69%) and TT broth (58.6%) were more effective than SC broth (24.1%). The chromogenic media gave better results than the classic ones with less false-positive colonies. The most effective isolation medium was CHROMagar, where Salmonella was identified in 23 (79.3%) of the 29 positive samples, followed by Rambach (48%). Positivity for Salmonella using classic media was 13.8% for BGA, 27.6% for SS and 34.5% for XLD.

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“…Though a rapid destruction occurred in the first 12 weeks of storage, it was followed by a reduced rate of reduction at 35-25 0 C, reduced Salmonella numbers were obtained in 4-8 weeks, such storage would not make heavily contaminated powders Salmonella negative in 8 weeks. Beckers et al [23] also worked on the suitability of artificially contaminated milk powder as a substrate for Salmonella reference sample and their stability under different storage temperature, especially at 4 0 C, they found milk powder as a suitable material for reference sample through a reconstitution steps were needed to be included in the standard Salmonella isolation procedure.…”

Section: Discussionmentioning

confidence: 99%

Pevalence of Samonella Species Isolated From Powdered Infant Formula Sold in Nnewi Market

Barbara¹,

Chinwe²,

Chinenye³

et al. 2019

SJPM

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The continual development of Novel molecular based technologies for rapid high detection of food-borne pathogenic bacteria has made the future of conventional microbiological isolation methods termingly tenous. Report by Salmonella species in children is also increasing. A study on prevalence of Salmonella in PIF was conducted. A total of 10 Powdered Infant Formula consisting of 9 foreign and 1 local brand were analysed using a total sample size of 80 units.100 milk rating was done by methylene-blue reductase test, coliform analysis by serial dilution method, salmonella isolation by Bacteriological Analytical Manual(BAM) method and membrane filtration methods, while antimicrobial susceptibility by disc diffusion method. Reports obtained revealed that 80% (8) of the milk sampled belong to class 1, while 20% (2) belong to class 2. Total coliform count of different dilutions of the 10 brands sampled contained acceptable limits of coliform contamination per gram of milk. SMA infant contained lesser coliform at the higher dilutions 10-3 , 10-2 than others, 3 bacteria isolated from the 10 brands of milk include, Salmonella species 54 (67.5%); (S.typhi, S.Paratyphi A,S. Paratyphi C,S. entericaand S.typhimorium), E. coli 25 (31.25%) and Proteus 1 (1.25). 5 different salmonella species were isolated at different frequency using BAM and membrane filtration methods. S. paratyphi C 11(36.7%) and S. paratyphi A 14 (58.3%) were isolated using BAM and membrane filtration methods. BAM method isolated a total frequency of 30(100%). Salmonella, while membrane filtration 24(100%) Salmonella species. BAM method showed the highest recovery for Salmonella specie than membrane filtration method. Comparison of recovery rate between the two methods showed a statistical difference between them (P<0.05). Antibiotic susceptibility test show that all Salmonella specie was sensitive to Ciprofloxacin at (10mg) 24 (44.46%) while penicillin (30mg) had the highest resistance 54 (100%) rate. A statistical significant difference was observed between resistant and sensitive antibodies (P<0.05). Some powdered infant Formula sold in Nnewi are contaminated with different Salmonella species especially S. Paratyphi C and though are of class1 grade, contained acceptable coliform units/g og milk sample. Recovery rate of pathogen is best when performed with BAM method.

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Evaluation of reference samples for the detection of Salmonella

Cited by 13 publications

References 8 publications

Collaborative study on the isolation of salmonella from artificially contaminated milk powder

Collaborative study on the isolation of salmonella from artificially contaminated milk powder

Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry

Pevalence of Samonella Species Isolated From Powdered Infant Formula Sold in Nnewi Market

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