Evaluation of renal ischemia‐reperfusion injury by magnetic resonance imaging texture analysis: An experimental study

Abstract

To explore the value of MRI texture analysis in evaluating the presence and severity of early renal ischemia-reperfusion injury (IRI). Methods: Healthy New Zealand rabbits were used (IRI group, N = 54; control group, N = 8). Rabbits in the IRI group underwent left renal artery clamping for 60 minutes. Magnetic resonance imaging was performed before and at 1, 12, 24, and 48 hours after IRI. The relationship between MRI texture features and histopathology parameters was assessed using Pearson's correlation coefficients. The diagnostic performance of texture features in kidney differentiation at different time points was assessed by receiver operating characteristic curve analysis. Results: T 2 WI_S(3,-3)Inverse_Difference_Moment had the strongest correlation with brush border destruction, tubular epithelial edema, necrosis, and cast (r = 0.56, −0.58, 0.62, and 0.69, respectively; all P < .001). BOLD_S(4,-4)Correlation had the strongest correlation with interstitial inflammatory cell infiltration (r = 0.63, P < .001). SWI_S(4,4)Difference_Entropy had the strongest correlation with microvessel density (r = 0.61, P < .001). The areas under the curve for T 2 WI_S(3,-3) Inverse_Difference_Moment, SWI_S(4,4)Difference_Entropy, and BOLD_S(4,-4) Correlation in kidney differentiation before IRI and that at 1 and 12 hours after reperfusion were 0.76, 0.72, and 0.70, respectively; the values before IRI and at 24 and 48 hours after reperfusion were 0.84, 0.81, and 0.69, respectively. The area under the curve for T 2 WI_S(3,-3)Inverse_Difference_Moment in kidney differentiation at 1 and 12 hours after reperfusion and that at 24 and 48 hours after reperfusion was 0.66. Conclusion: Magnetic resonance imaging texture analysis can be used for evaluating the presence and severity of early renal IRI.

Perfusion Analysis of Kidney Injury in Rats With Cirrhosis Induced by Common Bile Duct Ligation Using Arterial Spin Labeling MRI

“…34 A possible explanation may be the different evaluation criteria for renal injury. We used the Paller criteria 19,20 to determine tubule dilation, brush border destruction, vacuole degeneration, interstitial edema, inflammatory cell infiltration, tube casting, and nuclear pyknosis. In contrast, they considered the presence of significant tubular necrosis as a definitive indication of renal injury.…”

“…The liver fibrosis staging was classified according to the METAVIR scoring system 18 as: S0, no fibrosis; S1: enlarged fibrous hyperplasia in the portal area with limited perisinusoidal and intralobular fibrosis; S2, peripheral fibrosis in the portal area with formation of fibrous septa and structural intact lobules of the liver; S3: fibrous septa with intralobular structural disturbances but no cirrhosis; and S4, definite cirrhosis. Under the microscope, five randomly selected nonoverlapping areas in each renal compartment were used to sum the scores for each pathological change (tubule dilation, brush border destruction, vacuole degeneration, interstitial edema, inflammatory cell infiltration, tube casting, and nuclear pyknosis) to obtain total severity scores for cortex (CO) 19,20 . Semi‐quantitative image profiling was conducted using the open‐source software ImageJ and the IHC profiler plug‐in initiated by Varghese et al 21 Three fields were freely selected to calculate the gross score to reflect HIF‐1α expression.…”

“…Under the microscope, five randomly selected nonoverlapping areas in each renal compartment were used to sum the scores for each pathological change (tubule dilation, brush border destruction, vacuole degeneration, interstitial edema, inflammatory cell infiltration, tube casting, and nuclear pyknosis) to obtain total severity scores for cortex (CO). 19,20 Semi-quantitative image profiling was conducted using the open-source software ImageJ and the IHC profiler plug-in initiated by Varghese et al 21 Three fields were freely selected to calculate the gross score to reflect HIF-1α expression. PTC density was inspected with positively CD34-stained sections and tallied by censoring three random views in each section, and capillary density was denoted as the mean number of capillaries/0.065 mm 2 .…”

“…The tubules suffered varying degrees of damage (Fig. 5) according to the Paller criteria, 19,20 most notably on the 3rd, 28th, and 42nd days (Fig. 4g).…”

Evaluating renal iron overload in diabetes mellitus by blood oxygen level-dependent magnetic resonance imaging: a longitudinal experimental study

“…Moreover, R 2 * values of BOLD MRI were related to the level of renal oxygenation, and indirectly re ected the degree of kidney brosis and injury [9,10]. We hypothesized that BOLD MRI can be not only used to assess kidney iron deposition but also to indirectly evaluate kidney injury caused by renal iron overload in diabetic mellitus.…”