Evaluation of Resistance Development in Bemisia tabaci Genn. (Homoptera: Aleyrodidae) in Cotton against Different Insecticides

Khalid, Muhammad Zaryab; Ahmed, Sohail; Al-Ashkar, Ibrahim; Sabagh, Ayman El; Liu, Liyun; Zhong, Guohua

doi:10.3390/insects12110996

Cited by 20 publications

(15 citation statements)

References 39 publications

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“…The silverleaf whitefly B. tabaci is no exception, with median lethal concentrations of pymetrozine being increased approximately 11 times after 18 generations (Wang et al 2021). The recent results provided by Khalid et al (2021) are even more striking, as these authors reported a very high increase of the resistance level to buprofezin (127-fold) and imidacloprid (86-fold) after five generations only. A masterpiece case of rapid adaptation was conducted by Karasov et al (2010) in Drosophila demonstrating that organophosphate treatments quickly induced ace point mutations, resulting in multiple simple and complex resistant-ace alleles in the investigated fly subpopulations.…”

Section: Discussionmentioning

confidence: 94%

Predicting the insecticide-driven mutations in a crop pest insect: Evidence for multiple polymorphisms of acetylcholinesterase gene with potential relevance for resistance to chemicals

Renault

Elfiky

Mohamed

2022

Environ Sci Pollut Res

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The silverleaf whitefly Bemisia tabaci (Gennadius, 1889) (Homoptera: Aleyrodidae) is a serious invasive herbivorous insect pest worldwide. The misuse and excessive use of pesticides hasve progressively selected B. tabaci specimens, progressively reducing the effectiveness of the treatments, and ultimately ending in the selection of pesticide-resistantce strains. The management of this crop pest has thus become challenging owing to the high levels of resistance to all major classes of recommended insecticides, including organophosphates (OPs). Here, we used in silico techniques for detecting molecular mutations in ace1 gene from naturally occurring B. tabaci variants, and monitor the presence and frequency of the detected mutations from 30 populations of the silver whitefly from Egypt and Pakistan. We found several point mutations in ace1-type acetylcholinesterase (ace1) in the studied B. tabaci B variants (MEAM1) naturally occurring in the field. By comparing ace1 sequence data and point mutations with those reportedfrom on an organophosphate-susceptible and an organophosphate-resistant strains of B. tabaci (SUD-S), and to ace1 sequence data retrieved from GenBank for that species and to nucleotide polymorphisms from other arthropods, we identified novel mutations that could potentially influence insecticide resistance. Seven Egyptian field strains had 100% homology with the fragment of the Israeli B. tabaci susceptible allele (SUD-SUD), revealing a high conservation of the sequence/nucleotide, and/or possible missense mutations. We also report novel amino acid substitutions (S369W, C316W, P317G, and P317F) as likely candidates conferring resistance to organophosphates. Indeed, the location of these mutations, which we visualized on the 3D crystal structure of acetylcholinesterase, occur lose to the access of the active site gorge of the enzyme. The phylogenetic analysis revealed that these novel gene sequences appeared at the top of the clade in the tree, suggesting that the reported novel point mutations have evolved recentlyHomology modeling and molecular docking analyses were performed to determine if the mutation-induced changes in form 1 acetylcholinesterase (AChE1) structure could confer resistance to carbamate and organophosphate insecticides. Mutations had small effects on binding energy (ΔGb) interactions between mutant AChE1 and insecticides; they altered the conformation of the peripheral anionic site of AChE1, and modified the enzyme surface, and these changes have potential effects on the target-site sensitivity. Altogether, the results from this study provides information on genic variants of B. tabaci ace1 for future monitoring insecticide resistance development and report a potential case of environmentally driven gene variations.

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Section: Discussionmentioning

confidence: 94%

Predicting the insecticide-driven mutations in a crop pest insect: Evidence for multiple polymorphisms of acetylcholinesterase gene with potential relevance for resistance to chemicals

Renault

Elfiky

Mohamed

2022

Environ Sci Pollut Res

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“…The possible reasons in Pakistan scenarios, lack of speci ed resistance management schemes and injudicious use of insecticides are supposed to be the reasons in development of resistance in B. tabaci (Banerjee et al, 2014). The continuous relying on limited group of insecticides has lead and resulted in resistance development to B. tabaci (Khalid et al, 2021a).…”

Section: Resistance Monitoring Resultsmentioning

confidence: 99%

Insecticidal Resistance Monitoring in mitotypes of Bemisia tabaci in South Punjab region of Pakistan

Shamraiz,

Saeed,

Qayyum

et al. 2023

Preprint

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Whitefly (Bemisia tabaci Gennadius) (Hemiptera: Aleyrodidae) is a serious pest of several summer crops in hot and dry climates with diverse cryptic species complex worldwide. Among 7 major clades of B. tabaci; Asia-II is predominant in the cotton zone of Punjab-Pakistan. Cotton is one of the favorite hosts of B. tabaci, where it feeds and spread different plant viruses. In Pakistan, the primary approach used to manage B. tabaci in the cotton environment is through the use of synthetic pesticides. A vital tool for the effective management of B. tabaci is the monitoring of insecticidal resistance. Different cotton field strains were collected from the four major cotton growing districts of South-Punjab to assess their genetic variability and resistance levels compared with the laboratory susceptible population against most commonly used insecticides during the years 2020 and 2021. Leaf-dip bioassays were performed on 2nd instar nymphal (N2) stage; while adult bioassays were conducted on G1 stage on field collected populations. Genetic analysis of mtCOI revealed that all the populations were belonged to Asia II-1 clade. A mixture of three haplotypes of Asia II-I including haplotypes-2, haplotype-3 and haplotype-4 was identified from Jampur and Bahawalpur samples. However, haplotype-4 and haplotype-3 were identified from Multan population while Vehari samples comprised of haplotype-4 and haplotype-2. Buprofezin (RR ranged 25.75–36.71), pyriproxyfen (RR ranged 7.98–12.78) and diafenthiuron (RR ranged 20.59–28.12) were least efficient products in adult bioassays and had moderate to low level of resistance. However, spirotetramat and flonicamid both were relatively effective against adults with low to very low resistance during both the years. Pyriproxyfen demonstrated least efficacy for reducing adult emergence followed by flonicamid and buprofezin with moderate to very low resistance ratio respectively. In contrast spirotetramat (RR ranged 2.20–4.01), was highly effective against nymphs followed by diafenthiuron (RR ranged 4.63–7.68), having very low resistance ratio. However an upward trend of resistance development was observed against all the five tested insecticides during both the monitoring years. Current study concluded that different mitotypes/haplotypes of B. tabaci have various level of insecticidal resistance to the old conventional insecticides. These results establish a regional baseline that can serve as a reference for future monitoring and management of B. tabaci resistance to the tested insecticides.

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“…Judicious application of insecticides is essential (76), since a non-judicious and unnecessary use of pesticide has developed resistance in insect pests. Bemisia tabaci is the fifth most insecticide-resistant species in the world out of the top 12; therefore, the control of whitefly has become a global concern due to readily developing resistance against each new introduced insecticide (46). Indiscriminate use of insecticides against whitefly can only be stopped by the proper and timely pest scouting.…”

Section: Vector Management Through Insecticidesmentioning

confidence: 99%

Is the best resistance strategy against begomoviruses yet to come? A Comprehensive Review

Ishfaqe

Shafiq

Ali³

et al. 2022

Summa phytopathol.

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The worldwide spread of Geminiviruses and its vector has been attributed to the high rate of recombination, mutation, presence of alternate hosts, transport of plant material, global human activity along with global trade, new agricultural practices and climate change; therefore, the most severe viral diseases among crops have grown in almost all regions of the world, including cassava viral diseases in Africa, cotton leaf curl disease in Pakistan, and multiple diseases related to tomatoes, legumes and cucurbits in India and all over the world. Begomovirus is the major and largest genus of Geminiviridae which has shown a worldwide increase in its variations and distribution by affecting previously unaffected plants. The increasing population has augmented the demand for a sustainable agriculture boost, but begomoviruses have become a continual threat to the world’s agriculture. Numerous control strategies have been used for the viral invasion of the world as pathogens but begomoviruses continually overcome the control methods, and this has generated a need to end this competition between researchers and begomoviruses. The present review summarizes all strategies used against begomoviruses and provides a comprehensive comparison among all control methods, their drawbacks, and future insights.

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Evaluation of Resistance Development in Bemisia tabaci Genn. (Homoptera: Aleyrodidae) in Cotton against Different Insecticides

Cited by 20 publications

References 39 publications

Predicting the insecticide-driven mutations in a crop pest insect: Evidence for multiple polymorphisms of acetylcholinesterase gene with potential relevance for resistance to chemicals

Predicting the insecticide-driven mutations in a crop pest insect: Evidence for multiple polymorphisms of acetylcholinesterase gene with potential relevance for resistance to chemicals

Insecticidal Resistance Monitoring in mitotypes of Bemisia tabaci in South Punjab region of Pakistan

Is the best resistance strategy against begomoviruses yet to come? A Comprehensive Review

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