Up to 15 fumarolic gas samples were collected successively within a few hours at Satsuma-Iwojima and Kuju volcanoes, southwest Japan, with the aim to evaluate the naturally occurring short-term variations in the chemical and isotopic compositions. Variations in the concentrations of the major gas components (CO 2 , total S, SO 2 and H 2 S) and in the hydrogen, oxygen (H 2 O) and carbon (CO 2 ) isotopic compositions were relatively small and were within the analytical errors. Precision associated with the gas sampling and analytical techniques used in this study were estimated by making the hypothesis that the chemical and isotopic compositions of the fumarolic gases did not change during the sampling period. The estimated precision was 1-3% for CO 2 , H 2 S and SO 2 , 6-11% for HCl, 1-6% for H 2 , 4-6% for N 2 , 5-9% for Ar, 2% for CH 4 and 7-27% for CO. The precision of the isotopic analyses was estimated to be 0.05-0.2‰ for 13 C/ 12 C ratio of CO 2 . Also the precision was 0.9 and 0.05-0.11‰ for D/H and 18 O/ 16 O ratios of the condensates, respectively. The results in this study suggest that careful sampling and analysis of volcanic gases provide reliable chemical and isotopic compositions that can be used to monitor volcanic activities.