Evaluation of results of changes in bacterial strains in ecosystem of lake Baikal

Abstract

In the paper, we evaluated the results of changes in the microbial community using molecular research methods. The authors analyzed strains of microorganisms isolated from different parts of the ecosystem of Lake Baikal: pelagial, littoral and benthic sediments. While analyzing we identified the presence of restriction endonuclease (RE) enzymes. It was established that RE were detected in bacteria isolated from samples taken in places of anthropogenic influence (Listvyanka, Baikalsk). As a result, 28 types of different RE were identified. When reviewing the results obtained, it was established that the formation of these enzymes in bacteria is associated neither with the season of the year nor with the year of sampling. The condition for the synthesis of RE enzymes in microorganism strains is the appearance of allochthonous microflora in the lake ecosystem. These are the littoral parts of the lake, where anthropogenic influence is observed. As a result, it was established that the biosynthesis of RE enzymes occurred in bacterial strains under the influence of human activity. It is especially significant that anthropogenic changes can be identified in the early stages of impact on the ecosystem of Lake Baikal.