Evaluation of Reverse Passive Hemagglutination (RPHA) Fecal Occult Blood Test in screening of colorectal neoplasia

Yu, Hai; Zhou, Lun; Zheng, Beiyi; Qiu, Peilin; Sun, Qirong; Shao, Yuwen; Ma, Xiaoxin

doi:10.1007/bf03025581

Cited by 3 publications

(5 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, additional FOBTs and endoscopy were required to achieve adequate specificity and sensitivity in screening colorectal cancer (21). But almost one half of the colorectal cancer cases were FOBT negative, especially in early stages (4). In our result, we also find that CEA has a sensitivity of 47.3% and a specificity of 93.5% in screening for colorectal cancer with an upper limit of 5.0 ng/mL.…”

Section: Discussionmentioning

confidence: 48%

Artificial Neural Networks Analysis of Surface-Enhanced Laser Desorption/Ionization Mass Spectra of Serum Protein Pattern Distinguishes Colorectal Cancer from Healthy Population

Chen¹,

Zheng

et al. 2004

Clinical Cancer Research

Self Cite

133

View full text Add to dashboard Cite

Purpose:The low specificity and sensitivity of the carcinoembryonic antigen test makes it not an ideal biomarker for the detection of colorectal cancer. We developed and evaluated a proteomic approach for the simultaneous detection and analysis of multiple proteins for distinguishing individuals with colorectal cancer from healthy individuals.Experimental Design: We subjected serum samples (including 55 colorectal cancer patients and 92 age-and sexmatched healthy individuals) from 147 individuals, for analysis by surface-enhanced laser desorption/ionization (SELDI) mass spectrometry. Peaks were detected with Ciphergen SELDI software version 3.0. Using a multilayer artificial neural network with a back propagation algorithm, we developed a classifier for separating the colorectal cancer groups from the healthy groups.Results: The artificial neural network classifier separated the colorectal cancer from the healthy samples, with a sensitivity of 91% and specificity of 93%. Four top-scored peaks, at m/z of 5,911, 8,930, 8,817, and 4,476, were finally selected as the potential "fingerprints" for detection of colorectal cancer.Conclusions: The combination of SELDI-TOF mass spectrometry with the artificial neural networks in the analysis of serum protein yields significantly higher sensitivity and specificity values for the detection and diagnosis of colorectal cancer.

show abstract

Section: Discussionmentioning

confidence: 48%

Artificial Neural Networks Analysis of Surface-Enhanced Laser Desorption/Ionization Mass Spectra of Serum Protein Pattern Distinguishes Colorectal Cancer from Healthy Population

Chen¹,

Zheng

et al. 2004

Clinical Cancer Research

Self Cite

133

View full text Add to dashboard Cite

show abstract

“…However, when the three sampling method is used with taking 1/3 (+) as positive criteria, 22.0% of colorectal neoplasias can be detected. The authors previously reported that the sensitivity of three sampling RPHA FOBT for colorectal cancer was 63.6% and was 40% for villous or tubulovillous adenoma, which has an increasing tendency to malignant transformation [8] . The low sensitivity of one sampling RPHA FOBT may result from the variation of bleeding status of early colorectal neoplasia, particularly polyps.…”

Section: Discussionmentioning

confidence: 99%

“…The bowel preparation and endoscopic procedures were reported in another paper [4] . No dietary control was required and three day fecal samples were collected and submitted for laboratory testing before endoscopy.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of a fecal occult blood test with reverse passive hemagglutination for colorectal neoplasm screening

Zhou¹,

Yu²

2011

WJG

Self Cite

View full text Add to dashboard Cite

AIM:To evaluate the one and three sampling reverse passive hemagglutination fecal occult blood test (RPHA FOBT) for colorectal neoplasm screening. METHODS:A group of 3034 individuals with histories of colorectal polyps and/or ulcers were screened for colorectal cancer. Three day fecal samples were collected and 60 cm ��beroptic colonoscopy was conducted for each subject. The fecal samples were tested for occult blood with the RPHA method and the endoscopic and histopathological diagnoses were used as standard reference for evaluation. The sensitivity, specificity and positive and negative predictive values of different samplings were compared. RESULTS:About 521 cases of colorectal neoplasms were detected, including 12 cases of colorectal cancer and 50� cases of polyps. Results showed that the mean sensitivity of one sampling RPHA FOBT for colorectal neoplasm was only 13.2%, the specificity was �0.3% and the positive and negative predictive values were 21.3% and 83.4%, respectively; while for the three sampling, taking one positivity as positive, the sensitivity increased to 22.0%, the specificity decreased to 81.6% and the positive and negative predictive values were 1�.7% and 83.6%, respectively. CONCLUSION:A single RPHA FOBT seems to be less sensitive for screening for colorectal neoplasms. Since it is convenient and economical, RPHA FOBT remains the most practical procedure for detection of early colorectal cancer and polyps if it is combined with other screening methods. Zhou L, Yu H, Zheng S. Evaluation of a fecal occult blood test with reverse passive hemagglutination for colorectal neoplasm screening.

show abstract

“…Hope et al 17 concluded that the monoclonal antibody method, an FOBT that uses a monoclonal antibody specific to human hemoglobin, is a more accurate test in the detection of colorectal neoplasia and should be used in colorectal cancer screening programs. Unfortunately, as Yu and colleagues report, 9 only half of all colorectal cancers detected produce blood in the stool, with the remaining cancers producing false-negative results with the use of FOBT alone. In the present study, quantitative risk assessment (ADV) was used with FOBT and reduced the number of false-negative results, because the procedure of ADV has a higher sensitivity (70.2 percent) than the FOBT.…”

mentioning

confidence: 92%

“…Moreover, we have reported that only half of all colorectal cancers produced blood in the stool. 9 Thus, FOBT-based screening alone would miss approximately half of all colorectal cancers.…”

mentioning

confidence: 99%

Cluster Randomization Trial of Sequence Mass Screening for Colorectal Cancer

Zheng¹,

Chen²,

Liu³

et al. 2003

Diseases of the Colon &Amp; Rectum

Self Cite

View full text Add to dashboard Cite

show abstract

Evaluation of Reverse Passive Hemagglutination (RPHA) Fecal Occult Blood Test in screening of colorectal neoplasia

Cited by 3 publications

References 5 publications

Artificial Neural Networks Analysis of Surface-Enhanced Laser Desorption/Ionization Mass Spectra of Serum Protein Pattern Distinguishes Colorectal Cancer from Healthy Population

Artificial Neural Networks Analysis of Surface-Enhanced Laser Desorption/Ionization Mass Spectra of Serum Protein Pattern Distinguishes Colorectal Cancer from Healthy Population

Evaluation of a fecal occult blood test with reverse passive hemagglutination for colorectal neoplasm screening

Cluster Randomization Trial of Sequence Mass Screening for Colorectal Cancer

Contact Info

Product

Resources

About