Evaluation of Rhodotorula spp. contamination in hospital environments

Sanna, Clara; Marras, Luisa; Desogus, Alessandra; Marras, Barbara; Montero, Natalia; Bertolino, Giacomo; Schintu, Marco; Coroneo, Valentina

doi:10.1007/s10661-021-08908-3

Cited by 5 publications

(4 citation statements)

References 23 publications

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“…Similarly, other studies have reported Rhodotorula sp. as an indoor air contaminant in critical and semicritical hospital areas [48,49]. Equally, Souza et al [43] evaluated fungal contamination of indoor air in two neonatal intensive care units and reported R. mucilaginosa.…”

Section: Discussionmentioning

confidence: 99%

Indoor Air Contamination by Yeasts in Healthcare Facilities: Risks of Invasive Fungal Infection

do Nascimento,

dos Santos,

dos Santos Silva

et al. 2023

Aerobiology

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Introduction-Aims: Fungi are ubiquitous microorganisms that are easily dispersed through the air. In healthcare environments, indoor air can favor the spread of healthcare-associated fungal infections, compromising mainly immunocompromised hospitalized individuals. Therefore, this study aimed to evaluate indoor air contamination in healthcare environments, investigating mainly the presence of potentially pathogenic yeasts. Method: Indoor air samples were collected from 12 healthcare environments (hospital and medical clinics). After the growth, isolation, and purification of the yeast colonies, the isolates were identified by polymerase chain reaction using species-specific primers for yeasts of the genus Candida and sequencing of D1/D2 domains of the large ribosomal subunit (LSU rRNA). Results and interpretation: Fourteen yeast species were identified, including emerging pathogens. Species of clinical importance such as Candida parapsilosis, Candida glabrata, and Rhodotorula mucilaginosa were present. C. parapsilosis was the most prevalent species, followed by Rhodotorula mucilaginosa. In addition, we report the first occurrence of Candida orthopsilosis, Trichosporon mucoides, Fereydounia khargensis, and Hortaea werneckii in indoor air samples collected in healthcare environments. The present study shows that potentially fungal pathogens were present in air samples from healthcare environments, proving the role of indoor air in spreading infections. Therefore, monitoring air quality in healthcare environments is a fundamental approach to developing infection control measures, especially those related to invasive fungal infections.

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Section: Discussionmentioning

confidence: 99%

Indoor Air Contamination by Yeasts in Healthcare Facilities: Risks of Invasive Fungal Infection

do Nascimento,

dos Santos,

dos Santos Silva

et al. 2023

Aerobiology

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“…An amount of 4.70 g (yield 48%) of phthalide was obtained. Spectroscopic data: 1 3-n-butyl-3a,4,7,7a-tetrahydrophthalide (7) was prepared according to the procedure (steps 1 and 2a), and C 4 H 9 Br and 1,2,5,6-tetrahydrophthalic anhydride (7.7 g, 0.05 mole) were used as the substrate. An amount of 4.85 g (yield 50%) of phthalide was obtained.…”

Section: Synthesis Of Phthalide Lactonesmentioning

confidence: 99%

“…The following supporting information can be downloaded at: https://www. mdpi.com/article/10.3390/molecules28145423/s1, Figure S1a: 1 H NMR of 3-n-butylidenephthalide (1); Figure S1b: 13 C NMR of 3-n-butylidenephthalide (1); Figure S1c: HR-ESI-MS/MS of 3-nbutylidenephthalide (1); Figure S2a: 1 H NMR of 3-n-butylphthalide (2); Figure S2b: 13 C NMR of 3-n-butylphthalide (2); Figure S2c: HR-ESI-MS/MS of 3-n-butylphthalide (2); Figure S3: HR-ESI-MS/MS of 3-n-propylidenephthalide (3); Figure S4a: 1 H NMR of 3-n-propylphthalide (4); Figure S4b: 13 C NMR of 3-n-propylphthalide (4); Figure S4c: HR-ESI-MS/MS of 3-n-propylphthalide (4); Figure S5a: 1 H NMR of 3-butyl-4,5,6,7-tetrahydrophthalide (5); Figure S5b: 13 C NMR of 3-Butyl-4,5,6,7-tetrahydrophthalide (5); Figure S5c: HR-ESI-MS/MS of 3-Butyl-4,5,6,7-tetrahydrophthalide (5); Figure S6a: 1 H NMR of 3-n-butyl-hexahydrophthalide (6); Figure S6b: 13 C NMR of 3-n-butylhexahydrophthalide (6); Figure S6c: HR-ESI-MS/MS of 3-n-butyl-hexahydrophthalide (6); Figure S7a: 1 H NMR of 3-n-butyl-1,2,6,7-tetrahydrophthalide (7); Figure S7b: b 13 C NMR of 3-n-butyl-1,2,6,7tetrahydrophthalide (7); Figure S7c: HR-ESI-MS/MS of 3-n-Butyl-1,2,6,7-tetrahydrophthalide (7).…”

Section: Supplementary Materialsmentioning

confidence: 99%

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Fungistatic Effect of Phthalide Lactones on Rhodotorula mucilaginosa

et al. 2023

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Currently, there is an increasing number of cases of fungal infections caused by opportunistic strains of the yeast Rhodotorula mucilaginosa, mainly in immunocompromised patients during hospitalization. The excessive use of antibiotics and azole compounds increases the risk of resistance to microorganisms. A new alternative to these drugs may be synthetic phthalide lactones with a structure identical to or similar to the natural ones found in celery plants, which show low toxicity and relatively high fungistatic activity. In the present study, the fungistatic activity of seven phthalide lactones was determined against R. mucilaginosa IHEM 18459. We showed that 3-n-butylidenephthalide, the most potent compound selected in the microdilution test, caused a dose-dependent decrease in dry yeast biomass. Phthalide accumulated in yeast cells and contributed to an increase in reactive oxygen species content. The synergistic effect of fluconazole resulted in a reduction in the azole concentration required for yeast inhibition. We observed changes in the color of the yeast cultures; thus, we conducted experiments to prove that the carotenoid profile was altered. The addition of lactones also triggered a decline in fatty acid methyl esters.

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