Evaluation of Rifampicin-resistant Tuberculosis in Pediatric Patients by GeneXpert MTB/RIF

William, Ashish; Rai, Yogita; Kaur, Ravinder

doi:10.5799/jmid.951506

Cited by 4 publications

(4 citation statements)

References 19 publications

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“…Overall, this study's MTB and rifampicin-resistant TB frequency was 18.98% and 7.11%, respectively. The incidence rate of M. tuberculosis (18.33%) in this study was higher than the previous study that reported 6.5% [18] and 7.9% [19] in Ethiopia, 13.8% [20] in Nepal, and 12% [21] in India. The incidence rate of M. tuberculosis infection among total suspects (n = 39,107) tested for GeneXpert MTB/RIF was 18.98% (7156/37,695), which is lower than the previous study that reported 38.77% [22] in China, 22.65% [23] and 22.9% [24] in Nigeria, and 23.82% [25] in India.…”

Section: Discussioncontrasting

confidence: 89%

Assess the Diagnostic Accuracy of GeneXpert to Detect Mycobacterium tuberculosis and Rifampicin-Resistant Tuberculosis among Presumptive Tuberculosis and Presumptive Drug Resistant Tuberculosis Patients

Ramachandra,

Brammacharry,

Muralidhar

et al. 2023

Microbiology Research

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GeneXpert MTB/RIF is a rapid molecular diagnostic tool capable of simultaneously detecting Mycobacterium tuberculosis and rifampicin resistance. This study aimed to assess the diagnostic precision of GeneXpert MTB/RIF assay to detect pulmonary and extrapulmonary tuberculosis and evaluate the performance for detecting of rifampicin resistance. Of 37,695 samples, 7156 (18.98%) were tuberculosis-positive, and 509 (7.11%) were rifampicin-resistant. The sensitivity, specificity, positive predictive value, negative predictive value, disease prevalence, and accuracy of the GeneXpert MTB/RIF assay for pulmonary tuberculosis were 99.87% (95%CI: 99.75–99.94), 99.92% (95%CI: 99.88–99.95), 99.71% (95%CI: 99.54–99.82), 99.97% (95%CI: 99.93–99.98), 21.38% (95%CI: 20.92–21.86), and 99.91% (95%CI: 99.87–99.94), respectively. For extrapulmonary tuberculosis, the sensitivity, specificity, PPV, NPV, disease prevalence, and accuracy of GeneXpert MTB/RIF assay accounted for 99.45% (95%CI: 98.73–99.82), 99.84% (95%CI: 99.73–99.92), 98.70% (95%CI: 97.73–99.25), 99.93% (95%CI: 99.84–99.97), 10.64% (95%CI: 9.99–11.31), and 99.80% (95%CI: 99.68–99.88), respectively. Despite its high sensitivity for detecting tuberculosis and rifampicin resistance, GeneXpert MTB/RIF had contradictory results for 20.5% of cases among patients with smear-negative results and 54.9% of cases among patients with a high risk of multidrug-resistant tuberculosis. Of 46% fluoroquinolone-resistant cases, 16.56% (26/157) were multidrug-resistant tuberculosis isolates, and 4.02% (20/498) were isoniazid-resistant, a characteristic distribution leading to about 17.2% of fluoroquinolone-resistance events and relevant marker gyr-A mutations in MDR tuberculosis isolates. Further, our study indicated that increased fluoroquinolone resistance among rifampicin-resistant and isoniazid-resistant tuberculosis endangers the success of newly endorsed MDR-TB regimens.

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Section: Discussioncontrasting

confidence: 89%

Assess the Diagnostic Accuracy of GeneXpert to Detect Mycobacterium tuberculosis and Rifampicin-Resistant Tuberculosis among Presumptive Tuberculosis and Presumptive Drug Resistant Tuberculosis Patients

Ramachandra,

Brammacharry,

Muralidhar

et al. 2023

Microbiology Research

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show abstract

“…Overall, this study's MTB and Rifampicin Resistant TB frequency was 18.33% and 7.11%, respectively. The incidence rate of M. tuberculosis (18.33%) in this study was higher than the previous study reported 6.5% [14] and 7.9% [15] in Ethiopia, 13.8% [16] in Nepal, and 12% [17] in India. The incidence rate of M. tuberculosis infection among total suspects (n=39107) tested for Xpert MTB/RIF was 18.33% (7156/39107), which is lower than the previous study reported 38.77% [18] in China, 22.65 % [19], 22.9% [20] in Nigeria and 23.82% [21] in India.…”

Section: Discussioncontrasting

confidence: 85%

Access the Diagnostic Accuracy of GeneXpert to Detect <em>Mycobacterium tuberculosis</em> and Rifampicin-Resistance Tuberculosis among Presumptive Tuberculosis and Presumptive Drug Resistant Tuberculosis Patients

Ramachandra,

Brammacharry,

Muralidhar

et al. 2023

Preprint

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Xpert MTB/RIF is rapid molecular diagnostic tool capable of simultaneously detecting Mycobacterium tuberculosis and rifampicin resistance. This study aimed to access the diagnostic precision of Xpert MTB/RIF assay to detect pulmonary and extra-pulmonary tuberculosis and to evaluate the performance for the detection of rifampicin resistance. Of 37695 samples, 7156(18.98%) were tuberculosis positive, 509(7.11%) were rifampicin-resistant. The sensitivity, specificity, PPV, NPV, Diseases prevalence and accuracy of Xpert MTB/RIF assay for PTB were 99.87% (95%CI:99.75-99.94), 99.92%(95%CI:99.88-99.95), 99.71%(95%CI:99.54-99.82),99.97%(95%CI:99.93-99.98),21.38% (95%CI:20.92-21.86),and 99.91%(95%CI:99.87-99.94), respectively. For EPTB, the sensitivity, specificity, PPV, NPV, Diseases prevalence and accuracy of Xpert MTB/RIF assay accounted for 99.45% (95%CI:98.73-99.82),99.84%(95%CI:99.73-99.92),98.70%(95%CI:97.73-99.25),99.93% (95%CI:99.84-99.97),10.64%(95%CI:9.99-11.31),and 99.80%(95%CI:99.68-99.88), respectively. Despite its high sensitivity for detecting tuberculosis and rifampicin resistance, Xpert MTB/RIF had contradictory results for 20.5% of cases among patients with negative smear results and 54.9% of cases among patients with a high risk of Multidrug-resistant tuberculosis. Of 47 fluoroquinolone-resistant, 16.56% (26/157) of Multidrug-resistant tuberculosis isolates and 4.02% (20/498) isoniazid-resistant are fluoroquinolone-resistant, a characteristic distribution leading to about 17.2% of fluoroquinolone resistance events and relevant marker gyr-A mutations in MDR tuberculosis isolates. Further, our study indicated that increased fluoroquinolone resistance among Rifampicin-resistant and isoniazid-resistant tuberculosis endangers the success of newly endorsed MDR-TB regimens.

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“…7 Acid-fast bacilli have a beaded look and are bright red. 13 The specimen was inoculated with the culture medium and allowed to grow at 37°C for 4 weeks. 6…”

Section: Smear Microscopy and Culturementioning

confidence: 99%

“…7 The systems documented the presence or absence of M. tuberculosis after two hours, classifying the bacterial load as very low, low, medium, or high as well as the subject's susceptibility to the antibiotic rifampicin. 13 We obtained the values of sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) by using the formulas mentioned as follows:…”

Section: Genexpert Mycobacterium Tuberculosis/resistance To Rifampinmentioning

confidence: 99%

Comparative Evaluation of Cartridge-based Nucleic Acid Amplification Test Smear Microscopy and Conventional Culture Techniques in Laboratory Diagnosis of Tuberculosis

Rattan,

Naseem,

Naaz

et al. 2024

The Indian Journal of Chest Diseases and Allied Sciences

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Aim: This study compared the nucleic acid amplification assay with smear microscopy and culture to assess the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) in patients with suspected pulmonary and extrapulmonary tuberculosis (TB) using pulmonary and extrapulmonary samples. Methods: The information for this retrospective study was collected from the Path Kind Laboratory-a National Accreditation Board for Testing and Calibration Laboratories (NABL) accredited lab in Gurugram, Haryana, India. We evaluated 1,520 samples with suspected TB for smear microscopy, culture, and GeneXpert between January 2019 and December 2020. These samples came from the pulmonary and extrapulmonary areas. Smear microscopy and GeneXpert's diagnostic capabilities for pulmonary and extrapulmonary TB were calculated, with Mycobacterium tuberculosis (MTB) culture from pulmonary and extrapulmonary specimens serving as the gold standard. Results: This study comprised 1,520 clinical samples altogether. Of these, 624 were extrapulmonary specimens, while 896 were pulmonary samples. Overall, acid-fast bacilli (AFB) smear microscopy's sensitivity, specificity,

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Evaluation of Rifampicin-resistant Tuberculosis in Pediatric Patients by GeneXpert MTB/RIF

Cited by 4 publications

References 19 publications

Assess the Diagnostic Accuracy of GeneXpert to Detect Mycobacterium tuberculosis and Rifampicin-Resistant Tuberculosis among Presumptive Tuberculosis and Presumptive Drug Resistant Tuberculosis Patients

Assess the Diagnostic Accuracy of GeneXpert to Detect Mycobacterium tuberculosis and Rifampicin-Resistant Tuberculosis among Presumptive Tuberculosis and Presumptive Drug Resistant Tuberculosis Patients

Access the Diagnostic Accuracy of GeneXpert to Detect <em>Mycobacterium tuberculosis</em> and Rifampicin-Resistance Tuberculosis among Presumptive Tuberculosis and Presumptive Drug Resistant Tuberculosis Patients

Comparative Evaluation of Cartridge-based Nucleic Acid Amplification Test Smear Microscopy and Conventional Culture Techniques in Laboratory Diagnosis of Tuberculosis

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