Evaluation of Rosmarinus officinalis Leaves Essential Oils Activity Against Vancomycin Intermediate Staphylococcus aureus (VISA) Isolated from Baghdad Hospital Patients

Abstract

Rosemary is a well-known aromatic and medicinal plant used to treat various ailments. This study evaluated Rosmarinus officinalis essential oil for its phytochemical and antibacterial properties. The essential oil was analysed by using a gas chromatography-mass Spectrometry (GC-MS) that revealed the common chemicals containing verbenone 36.20% and 1,8-cineol (Eucalyptol) 12.14%. Extracted essential oils were tested for antibacterial activity against vancomycin intermediate Staphylococcus aureus (VISA), a strain of bacteria obtained locally from bacteremia patients. Three isolates were found to be VISA positive using the E-test (strips) and the population analysis profile method (PAP). VISA showed lower resistance to the essential oils (MIC: Minimum inhibitory concentration, MIC = 1.5 mg/ml). Overall, the genetic influence of VISA was evaluated by looking at the hld gene expression after being isolated from blood samples and RNA extraction for qRT-PCR to determine hld gene expression. R. officinalis gene expression was analysed and calculated by using real-time PCR of RNA which revealed that it had decreased.

The Effect of Adding Different Proportions of Local Moringa oleifera Seed Oil on the Shelf Life Extension of Flaxseed Oil

“…The spectrum of the unidentified components was compared to the spectrum of the identified components stored in the National Institute of Standards and Technology (NIST) library of standards [17,18].…”

“…Thiobarbituric acid value (TBA)Estimated according to the official method of the American Oil Chemist Society [18]. The TBA value was calculated according to the following equation: TBA value (mg malonaldehyde/kg Oil) = Optical absorption value at a wavelength of 350 nm × 7.6…”