Evaluation of Rouxiella badensis Subsp Acadiensis (Canan SV-53) as a Potential Probiotic Bacterium

Abstract

The advent of omic platforms revealed the significant benefits of probiotics in the prevention of many infectious diseases. This led to a growing interest in novel strains of probiotics endowed with health characteristics related to microbiome and immune modulation. Therefore, autochthonous bacteria in plant ecosystems might offer a good source for novel next-generation probiotics. The main objective of this study was to analyze the effect of Rouxiella badensis acadiensis Canan (R. acadiensis) a bacterium isolated from the blueberry biota, on the mammalian intestinal ecosystem and its potential as a probiotic microorganism. R. acadiensis, reinforced the intestinal epithelial barrier avoiding bacterial translocation from the gut to deep tissues, even after feeding BALB/c mice for a prolonged period of time. Moreover, diet supplementation with R. acadiensis led to increases in the number of Paneth cells, well as an increase in the antimicrobial peptide α defensin. The anti-bacterial effect of R. acadiensis against Staphylococcus aureus and Salmonella enterica serovar Typhimurium was also reported. Importantly, R. acadiensis-fed animals showed better survival in an in vivo Salmonella enterica serovar Typhimurium challenge compared with those that received a conventional diet. These results demonstrated that R. acadiensis possesses characteristics of a probiotic strain by contributing to the reinforcement and maintenance of intestinal homeostasis.

Lentinula edodes Cultured Extract and Rouxiella badensis subsp. acadiensis (Canan SV-53) Intake Alleviates Immune Deregulation and Inflammation by Modulating Signaling Pathways and Epigenetic Mechanisms

“…Evidence also suggests that AHCC exerts inhibitory activity on cancer stem cells by modulating miRNAs involved in immune evasion [52]. The probiotic SV-53 is a novel Gram-negative probiotic bacterium isolated from the microbiota of wild blueberry fruit which exerts significant immunomodulatory effects at the gut level by improving gut mucosal immunity and gut barrier integrity, regulating IgA, IL-10, and IL-6 secretion, modulating gut miRNA expression, and reducing the population of pathogenic bacteria such as Salmonella Typhimurium and Staphylococcus aureus in intestinal fluid [53][54][55][56].…”

Complete genome sequence of Australian soil bacterium Rouxiella badensis DAR84756 resolved with Oxford Nanopore long-read and Illumina sequences

“…Rouxiella badensis is a Gram-negative bacterium in the family Yersiniaceae ( 1 ) found associated with soil and plant ecosystems ( 2 ), with emerging applications including post-harvest preservation of berries ( 3 ), a human antidepressant probiotic ( 4 – 6 ), biosynthesis of L-asparaginase ( 7 ), and uncharacterized antibiotics ( 8 ). High-quality genome sequences, such as described here, allow improved identification of metabolic pathways and secondary metabolite prediction ( 9 , 10 ).…”