Evaluation of Russian sturgeon (Acipenser gueldenstaedtii) Semen Quality and Semen Cryopreservation

Igna, Violeta; Telea, Ada; Florea, Tiana; Popp, Roxana; Grozea, A.

doi:10.3390/ani12162153

Cited by 6 publications

(2 citation statements)

References 56 publications

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“…All the studies carried out so far have been aimed at increasing sperm viability/motility, that is, the fertility of sperm, after thawing. However, in the sperm cryopreservation study using methanol in Russian sturgeon, the motility value obtained after thawing sperm samples was found low (18-25%); in the fertilization study conducted with the same sperm samples, fertilization percentage was obtained as 72.67% [53].…”

Section: Sturgeon Sperm Cryopreservationmentioning

confidence: 90%

Cryopreservation Studies in Aquaculture from Past to Present: Scientific Techniques and Quality Controls for Commercial Applications

Ekici¹,

Yamaner²,

Demircan³

2023

Biomedical Engineering

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In this section, cryopreservation of fish genetic resources, which is one of the important applications to ensure the sustainability of genetic resources of freshwater fish species, is discussed. At the same time, information is provided about the possible sources of contamination that may be encountered during cryopreservation applications. In this context, the results of sperm, egg, and embryo cryopreservation studies of fish and their success and failure in applications were evaluated in addition to the process from past to present. Information is given about the contamination that may develop depending on the applications in the process of cryopreservation and dissolving processes, as well as the studies carried out to eliminate extracellular disease agents. In the section, in addition to the evaluation of the results of scientific studies, commercial companies that commercially carry out gamete cryopreservation applications are also included. The contamination that may develop depending on the applications in the process of cryopreservation and thawing processes, as well as the studies carried out to eliminate extracellular disease agents are mentioned.

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Section: Sturgeon Sperm Cryopreservationmentioning

confidence: 90%

Cryopreservation Studies in Aquaculture from Past to Present: Scientific Techniques and Quality Controls for Commercial Applications

Ekici¹,

Yamaner²,

Demircan³

2023

Biomedical Engineering

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“…In the case of fish reproductive cells, the process of low-temperature cryopreservation results in a significant decrease in cell temperature [15][16][17]. Particular attention is paid to salmon and sturgeon fish [18][19][20][21][22], of which the decline in population has reached alarming levels and threatens the complete extinction of these species.…”

Section: Introductionmentioning

confidence: 99%

Finite Element Modeling of Crystallization with Temperature Jump to Improve Cryopreservation of Fish Germ Cells

Matrosov,

Soloviev,

Ponomareva

et al. 2024

Processes

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This article is devoted to the further development of a viable technology for low-temperature cryopreservation of reproductive cells of sturgeon fish using acoustic–mechanical fields and intelligent control of the freezing process. Before vitrification begins, the piezoactuator acts on a mixture of cryoprotectant and reproductive cells. This promotes intensive mixing of the cryoprotector and its diffusion through the cell membrane. When vitrification is carried out directly, a phase transition phenomenon is observed, accompanied by crystal formation. This article presents a new mathematical model describing this process as developed by the authors. The corresponding boundary conditions are formulated. Numerical experiments were carried out using the finite element method. It has been established that during vitrification without the use of a cryoprotectant, a sharp temperature jump is observed at the front of the crystalline formation boundary. The use of a cryoprotectant leads to a slowdown in the process of crystal formation, that is, to a weakening of the effect of one of the most important cryoprotective factors. The comparison with full-scale experiments showed qualitative agreement with the experimental results, which indicates the adequacy of the proposed model. The results obtained can be used in the future during the vitrification process and the evaluation of the quality of cryofreezing. The application of a new methodological approach to methods of long-term preservation at low temperatures of the genetic and reproductive material of hydrobionts using acoustic and mechanical effects and an intelligent control module opens up great opportunities for the creation of new cost-effective biotechnologies that make it possible to make the transition to a new type of aquatic farms, increase the stability of aquaculture, in general, to make environmental protection measures to save rare and endangered species more effective.

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Modelling of Phase Transitions in the Process of Cryopreservation of Biological Material

Matrosov,

Soloviev,

Serebryanaya

et al. 2024

Springer Proceedings in Materials

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Evaluation of Russian sturgeon (Acipenser gueldenstaedtii) Semen Quality and Semen Cryopreservation

Cited by 6 publications

References 56 publications

Cryopreservation Studies in Aquaculture from Past to Present: Scientific Techniques and Quality Controls for Commercial Applications

Cryopreservation Studies in Aquaculture from Past to Present: Scientific Techniques and Quality Controls for Commercial Applications

Finite Element Modeling of Crystallization with Temperature Jump to Improve Cryopreservation of Fish Germ Cells

Modelling of Phase Transitions in the Process of Cryopreservation of Biological Material

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