Evaluation of safety and protection efficacy on cpxR and lon deleted mutant of Salmonella Gallinarum as a live vaccine candidate for fowl typhoid

Matsuda, Kiku; Chaudhari, Atul A.; Lee, John Hwa

doi:10.1016/j.vaccine.2010.11.039

Cited by 63 publications

(68 citation statements)

References 27 publications

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“…A number of studies have demonstrated that the expression of acquired resistance to fowl typhoid depends upon the combined humoral and cell-mediated immune response of the infected host (Rana and Kulshreshtha, 2006;Park et al, 2010;Matsuda et al, 2011). In the present study, we observed a significant induction of systemic IgG responses in the immunised groups, especially in chickens immunised with a combination of ghost cells with Montanide TM ISA 70 VG (Fig.…”

Section: Discussionsupporting

confidence: 61%

Evaluation of Salmonella Gallinarum ghost formulated with Montanide™ ISA 70 VG adjuvant as a vaccine against fowl typhoid

Jawale

Somsanith

et al. 2015

Acta Veterinaria Hungarica

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Escherichia coli heat-labile enterotoxin B subunit (LTB) protein is a potent adjuvant. Salmonella Gallinarum ghosts carrying LTB (S. Gallinarum-LTB ghosts) were genetically constructed using a plasmid, pJHL187-LTB, designed for the co-expression of the LTB and E lysis proteins. This study evaluates the immunopotentiating effects of Montanide TM ISA 70 VG on S. Gallinarum-LTB ghost vaccination against fowl typhoid. Five-week-old layer chickens were injected intramuscularly with sterile PBS (non-immunised control, Group A), S. Gallinarum-LTB ghost (Group B) or S. Gallinarum-LTB ghost emulsified with Montanide TM ISA 70 VG adjuvant (Group C). Chickens from both Groups B and C showed significant induction of antigen-specific systemic IgG response compared to controls; in addition, Group C showed enhanced induction of systemic IgG response compared to Group B. We observed significant induction of antigen-specific lymphocyte proliferative response and increased mRNA levels of Th1 cytokines (IFN-γ and IL2) in both Groups B and C. Furthermore, in the challenge experiment with a virulent strain of S. Gallinarum, Group C showed higher survival rates compared with other groups. These results indicate that vaccination with the S. Gallinarum-LTB ghost in combination with Montanide TM ISA 70 VG may enhance the protective immunity against fowl typhoid.

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Section: Discussionsupporting

confidence: 61%

Evaluation of Salmonella Gallinarum ghost formulated with Montanide™ ISA 70 VG adjuvant as a vaccine against fowl typhoid

Jawale

Somsanith

et al. 2015

Acta Veterinaria Hungarica

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“…centrifuging heparinized blood samples collected from the wing vein, and the intestinal wash samples were collected as described previously (24). An indirect ELISA was performed with an outer membrane protein (OMP) fraction extracted from JOL860 as described previously (17).…”

Section: Methodsmentioning

confidence: 99%

“…Lymphocyte proliferation assay. The lymphocyte proliferation assay was performed to determine lymphocyte activation and cell-mediated immune responses (23). The sonicated bacterial protein suspension prepared from JOL860 was as described previously (43).…”

Section: Methodsmentioning

confidence: 99%

Characterization of a Novel Inactivated Salmonella enterica Serovar Enteritidis Vaccine Candidate Generated Using a Modified cI857/λ P_R/GeneEExpression System

et al. 2012

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ABSTRACTA new strategy to develop an effective vaccine is essential to control food-borneSalmonella entericaserovar Enteritidis infections. Bacterial ghosts (BGs), which are nonliving, Gram-negative bacterial cell envelopes, are generated by expulsion of the cytoplasmic contents from bacterial cells through controlled expression using the modified cI857/λ PR/geneEexpression system. In the present study, the pJHL99 lysis plasmid carrying the mutated lambda pR37-cI857 repressor and PhiX174 lysis geneEwas constructed and transformed inS. Enteritidis to produce a BG. Temperature induction of the lysis gene cassette at 42°C revealed quantitative killing ofS. Enteritidis. TheS. Enteritidis ghost was characterized using scanning and transmission electron microscopy to visualize the transmembrane tunnel structure and loss of cytoplasmic materials, respectively. The efficacy of the BG as a vaccine candidate was evaluated in a chicken model using 60 10-day-old chickens, which were divided into four groups (n= 15), A, B, C, and D. Group A was designated as the nonimmunized control group, whereas the birds in groups B, C, and D were immunized via the intramuscular, subcutaneous, and oral routes, respectively. The chickens from all immunized groups showed significant increases in plasma IgG and intestinal secretory IgA levels. The lymphocyte proliferation response and CD3+CD4+and CD3+CD8+T cell subpopulations were also significantly increased in all immunized groups. The data indicate that both humoral and cell-mediated immune responses are robustly stimulated. Based on an examination of the protection efficacy measured by observations of gross lesions in the organs and bacterial recovery, the candidate vaccine can provide efficient protection against virulent challenge.

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“…Throughout the experimental period after challenge the clinical symptoms daily evaluated were decreased in all vaccinated birds, using two or three doses of LV, whilst in unvaccinated animals severe clinical symptoms preceded death, including anorexia, weakness, greenish diarrhea, loss of appetite, fluffed feather and fever. The use of a live attenuated strain of SG, with deletions on cpxR and lon genes, could also protect chickens against fowl typhoid, and only slight anorexia and depression were temporarily observed by the authors, while in the unvaccinated group, birds showed severe infection followed by mortality of 100% (MATSUDA et al, 2011b).…”

Section: Discussionmentioning

confidence: 95%

“…The attenuated strains are able to colonize internal organs, without clinical symptoms, however they induce an efficient immune response (DE PAIVA et al, 2009;LEE et al, 2005;SHEHATA et al, 2013). The use of LV prepared with attenuated strains of SG has showed good immunogenicity and capacity to induce a strong cellular and humoral immune response in experimental models (MATSUDA et al, 2011a;2011b;PENHA FILHO et al, 2012). Previous work from our group showed that the mutant strain SGΔcobSΔcbiA has had the potential to protect chickens against SG infection in experimental models (PENHA FILHO et al, 2010;2012).…”

Section: Palavras-chavementioning

confidence: 99%

Protection of chickens against fowl typhoid using field vaccine programs formulated with the live attenuated strain Salmonella Gallinarum ΔcobSΔcbiA

et al. 2018

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Salmonella enterica serovar Gallinarum biovar Gallinarum (SG) is a host-specific bacteria that causes the fowl typhoid (FT). This disease is highly pathogenic to commercial chickens, specially brown layers and breeders, causing acute septicemia followed by high morbidity and mortality. Vaccination is extensively adopted in the fields as a biosafety tool for prevention of isolated infections and outbreaks in commercial poultry flocks. The present study evaluated the use of an attenuated SG with deletions on genes cobS and cbiA (SGΔcobSΔcbiA) as a live vaccine, using vaccination schemes adjusted for field conditions. To this end, brown layers were used in two different experiments, to evaluate the long-term protection, necessary in the fields. The vaccination scheme on the first experiment consisted of two doses, the first at 4 th week-of-age and the booster dose at 8 th week-of-age with challenge at 16 th week-of-age with wild SG strain. On the second experiment, the vaccination was carried out by different routes using three doses of the live vaccine, at 4 th , 8 th and 12 th weeks-of-age, and the challenge was done at 20 th weeks-of-age. After the challenge, the mortality was recorded during 28 days, and the egg production (experiment 2) was evaluated and compared with the group of unvaccinated layers. In both experiments, the mortality was significantly reduced, and the egg production was not affected in vaccinated layer-hens. In summary, this study shows the efficacy and the protection of different vaccination schemes against FT that can be applied under field conditions in commercial poultry farms.

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Evaluation of safety and protection efficacy on cpxR and lon deleted mutant of Salmonella Gallinarum as a live vaccine candidate for fowl typhoid

Cited by 63 publications

References 27 publications

Evaluation of Salmonella Gallinarum ghost formulated with Montanide™ ISA 70 VG adjuvant as a vaccine against fowl typhoid

Evaluation of Salmonella Gallinarum ghost formulated with Montanide™ ISA 70 VG adjuvant as a vaccine against fowl typhoid

Characterization of a Novel Inactivated Salmonella enterica Serovar Enteritidis Vaccine Candidate Generated Using a Modified cI857/λ P_R/GeneEExpression System

Protection of chickens against fowl typhoid using field vaccine programs formulated with the live attenuated strain Salmonella Gallinarum ΔcobSΔcbiA

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Evaluation of safety and protection efficacy on cpxR and lon deleted mutant of Salmonella Gallinarum as a live vaccine candidate for fowl typhoid

Cited by 63 publications

References 27 publications

Evaluation of Salmonella Gallinarum ghost formulated with Montanide™ ISA 70 VG adjuvant as a vaccine against fowl typhoid

Evaluation of Salmonella Gallinarum ghost formulated with Montanide™ ISA 70 VG adjuvant as a vaccine against fowl typhoid

Characterization of a Novel Inactivated Salmonella enterica Serovar Enteritidis Vaccine Candidate Generated Using a Modified cI857/λ PR/GeneEExpression System

Protection of chickens against fowl typhoid using field vaccine programs formulated with the live attenuated strain Salmonella Gallinarum ΔcobSΔcbiA

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Characterization of a Novel Inactivated Salmonella enterica Serovar Enteritidis Vaccine Candidate Generated Using a Modified cI857/λ P_R/GeneEExpression System