Evaluation of Salicin Isolated from Salix subserrata as a Radioprotector against Gamma Irradiation Induced Ultrastructural and Electrophoretic Changes in Spleen Tissue in Rats

Abstract

The aim of this study was to investigate the radioprotective effect of salicin against irradiation effect on spleen tissue in male rats. Lipid peroxidation product (MDA) level was measured as thiobarbituric acid reactive substance. Ultrastructural examination was carried out in spleen tissue by scanning electron microscope (SEM). The polyacrylamide gel electrophoresis for native protein, lipoprotein and zymogram were carried out in spleen homogenate. As expected, salicin resisted the irradiation effect and declined the MDA level in spleen homogenate of all treated groups. The alterations which were occurred as a result of irradiation in the spleen tissue could not be detected microscopically but they were detected electrophoertically at levels of protein and isozymes. Salicin prevented the qualitative mutagenic effect of irradiation on the electrophoretic protein pattern in the irradiated salicin simultaneous treated group (SI = 0.73). It showed the highest protective effect against qualitative mutagenic irradiation effect in catalase pattern in irradiated salicin pre-treated group (SI = 0.80). It could not prevent the abnormalities occurred qualitatively and quantitatively as a result of irradiation in peroxidase pattern in all irradiated salicin treated groups. While the esterase pattern showed the same electrophoretic pattern in the all irradiated salicin treated groups. The results suggested the radioprotective ability of salicin against gamma irradiation effect on various ultrastructural and electrophoretic patterns in spleen tissue of male rats.

Effect of Salix alba leave extract on growth performance, antioxidant level and immune status of broiler chickens reared at high altitude cold desert

“…It has been reported that aqueous extract of S. alba leaves has marked antileukemic activity (El-Shemy et al 2003). Previous studies by Abdalla et al (2015) and Kader et al (2015) reported a reduction in oxidative stress marker MDA in the brain and spleen tissues of rats respectively, after administration of salicin extracted from S. alba leaves. It has been reported by Jadhav et al (2011) that addition of S. alba leaves in the lamb feed provided proper nutrition and did not create any adverse effect on lamb performance under high altitude climatic conditions of Ladakh.…”

Rational Design of Nanomaterials for Various Radiation‐Induced Diseases Prevention and Treatment

“…[ 154 ] It is well known that the spleen plays a vital role in immune functions because it is the largest peripheral lymphoid organ in the body, where alterations in spleen homeostasis affect immune responses. [ 155,156 ] Herein, the clinical symptoms of spleen injury induced by radiation are immune‐related diseases. Regarding the damage mechanism, Cole et al.…”

The radio-protective effects of n-Hexane extracts of Telfairia occidentalis Hook. f. and Cucumeropsis mannii Naud. seed oils on the liver of irradiated male Wistar rats

“…Even if considerable research studies on their dangerous consequences have been documented, humans cannot be fully exempted from absorbing rays intentionally or unintentionally in our daily activities (1). Among the ionizing radiations encountered by humans, one of the most harmful is the radiation from gamma rays which have large energy and a short wavelength (2). Gamma rays can break the barriers of living organisms during their transmission, transmit their radiant energy to biological molecules within the cells and induce oxidative stress by the production of reacting oxygen species (ROS) and free radicals (2)(3)(4).…”

“…Among the ionizing radiations encountered by humans, one of the most harmful is the radiation from gamma rays which have large energy and a short wavelength (2). Gamma rays can break the barriers of living organisms during their transmission, transmit their radiant energy to biological molecules within the cells and induce oxidative stress by the production of reacting oxygen species (ROS) and free radicals (2)(3)(4).…”

“…Free radicals have been implicated in the incidence, occurrence and distribution of most metabolic abnormalities such as compromised cell membranes integrity, spleen dysfunction, cancer of the lungs and various other malignancies (1)(2)(3). The cytotoxic effects induced by ionizing radiation in normal tissues are reduced or ameliorated through the administration of various organic compounds with therapeutic potential as well as the capability of scavenging the free radicals (4,5).…”