Evaluation of Salivary and Serum Anti-Helicobacter Pylori in Egyptian Patients with H. Pylori Related Gastric Disorders

El-Fakhfakh, Effat Abd El-Monem; Montasser, Mohamed F.; Khalifa, Reham

doi:10.12816/0006466

Cited by 8 publications

(6 citation statements)

References 19 publications

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“…In the present study, the salivary sensitivity and specificity for detection of H. pylori infection were 85% and 82% respectively, and serum sensitivity and specificity were 75% and 79% respectively, that all of them were acceptable. Our results showed 49.6% prevalence of H. pylori infection, which was less than 68.9% reported in Egypt ( 5 ). Hooshmand et al reported 50% prevalence of H. pylori infection which was in line with ours ( 14 ).…”

Section: Discussioncontrasting

confidence: 83%

“…El-Mekki et al found no significant difference between serum and salivary antibody levels regarding endoscopic findings, which was concordant with our study ( 23 ). In this research, serum and salivary antibodies for diagnosis of H. pylori were acceptable with no statistical difference, while El-Fakhfakh et al found that the salivary antibodies were more sensitive and specific than serum antibodies ( 5 ). Leal et al revealed no significant difference between sensitivity and specificity of serum and saliva which was consistent with this study ( 10 ).…”

Section: Discussionmentioning

confidence: 56%

“…Anti- H. pylori antibody measurement in serum, urine and saliva among children, is not reliable due to wide variety of sensitivity and specificity of tests ( 13 ). In a study, the sensitivity of salivary IgG against H. pylori was low ( 5 ) and in another investigation, the low salivary IgG specificity was reported ( 1 ). In another survey, the sensitivity and specificity of salivary anti- H. pylori IgG were acceptable ( 14 ).…”

Section: Introductionmentioning

confidence: 98%

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Comparison of the serum and salivary antibodies to detect gastric Helicobacter pylori infection in Kashan (Iran)

et al. 2017

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Background and aimHelicobacter pylori (H. pylori) is an important and common contagious human pathogen which may cause peptic ulcer and also gastric cancer. The definite diagnosis of it is made through invasive tests. Recently, non-invasive tests including serologic tests of serum and saliva have been conducted for diagnosis of H. pylori infection. In this research, the diagnostic values of serum and salivary serology were compared together to use salivary anti-H. pylori test as an alternative method in the future.MethodsDuring this prospective case-control study on patients who were candidates for endoscopy and gastric biopsy from March 2015 to April 2016 in Shahid Beheshti hospital, Kashan, Iran, serum and salivary samples were obtained for measurement of Immunoglobulin G (IgG) antibody levels against H. pylori by enzyme-linked immunosorbent assay (ELISA). Histopathology was the gold standard test. Statistical analysis was performed by SPSS software version 16. Statistical tests included Kolmogorov-Smirnov, independent-samples t-test, Chi-square, Mann-Whitney U, Kruskal-Wallis, McNemar and correlation.ResultsOf 123 patients, sixty-one patients (49.6%) were H. pylori-positive according to histology. The median levels of anti-H. pylori antibodies in serum (p<0.001) and saliva (p<0.001) of H. pylori-positive cases were significantly higher than H. pylori-negative cases. Sensitivity, specificity, positive likelihood ratio, negative likelihood ratio and accuracy of serologic tests in serum were 75%, 79%, 3.5, 0.3, 77% and for saliva were 85%, 82%, 4.7, 0.18, 84% respectively.ConclusionDiagnostic values of salivary ELISA are comparable to serum ELISA and can be used as an alternative modality for diagnosis of H. pylori infection.

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Section: Discussioncontrasting

confidence: 83%

Section: Discussionmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 98%

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Comparison of the serum and salivary antibodies to detect gastric Helicobacter pylori infection in Kashan (Iran)

et al. 2017

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“…pylori infection. [ 4 , 10 , 31 – 33 ] Also, in the present study, the detection of oral H . pylori was accomplished by the direct amplification of a portion of a specific gene of the bacteria: VacA .…”

Section: Discussionsupporting

confidence: 64%

Oral and Gastric Helicobacter Pylori: Effects and Associations

et al. 2015

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IntroductionThis study consisted in the comparison of the prevalence of Helicobacter pylori (H. pylori) present in the stomach and in saliva of a sample of Portuguese adolescents and the assessment of the association between H. pylori infection with socio-demographic variables and prevalence of dental caries.Materials and MethodsA cross-sectional study was designed including a sample of 447 adolescents aged 12 to 19 years old, attending a public school in Sátão, Portugal. A questionnaire about socio-demographic variables and oral health behaviors was applied. Gastric H. pylori infection was determined using the urease breath test (UBT). Saliva collection was obtained and DNA was extracted by Polymerase Chain Reaction (PCR) in order to detect the presence of oral H. pylori.ResultsThe prevalence of gastric H. pylori detected by UBT was 35.9%. Within the adolescents with a gastric UBT positive, only 1.9% were positive for oral H. pylori. The presence of gastric H. pylori was found to be associated with age (>15years, Odds ratio (OR)=1.64,95%CI=1.08-2.52), residence area (urban,OR=1.48,95%CI=1.03-2.29) and parents´ professional situation (unemployed,OR=1.22,95%CI=1.02-1.23). Among those with detected dental caries during the intra-oral observation, 37.4% were positive for gastric H. pylori and 40.2% negative for the same bacterial strain (p=0.3).ConclusionsThe oral cavity cannot be considered a reservoir for infection of H. pylori. Gastric H. pylori infection was found to be associated with socio-demographic variables such as age, residence area and socioeconomic status.

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“…Helicobacter pylori, a gram-negative bacterium found on the luminal surface of the gastric epithelium, was first isolated in 1983 (Warren et al,1983).Infection with H. pylori is a cofactor in the development of three important upper gastrointestinal diseases: duodenal or gastric ulcers (El-Omar et al,1995), gastric cancer (Hansen et al,1999), and gastric mucosa-associated lymphoid-tissue (MALT) lymphoma (Parsonnet et al,1994). It was thought that H. pylori pathogenic effects were limited to the stomach, however, its existence in extra gastric structures was reported (El-Fakhfakh et al, 2014). Helico-bacter pylori and H. bilis were detected in the bile and gallbladder in 75 % of patients with gallbladder cancer and more than 50% with chronic cholecystitis (Murphy et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Detection of Helicobacter Antigen in Stool Samples and Its Relation to H. Pylori Positive Cholecystitis in Egyptian Patients with Chronic Calcular Cholecystitis

Hassan¹,

Gerges²,

Ahmed³

2015

JESP

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Evidences supporting the association between H. pylori infection and chronic cholecystitis could be found by using direct culture or staining of H. pylori in gallbladder tissues as well as indirect techniques. Stool antigen test has been widely used due to its non-invasive nature. Various stool antigen tests were developed to detect H. pylori using an enzyme immunoassay (EIA) based on monoclonal or polyclonal antibodies This study evaluated the frequency of H. pylori antigen in stool samples of patients with chronic calcular cholecystitis as regard gall bladder histopathological changes.-Fifty patients were included presented with symptomatic cholecystolithiasis recruited from the outpatient clinic of National Hepatology and Tropical Medicine Research Institute during 2014-2015. Full history and clinical examination and abdominal ultrasonography were performed. Stool samples were collected, prepared and examined for detection of H. pylori antigen. Cholecystectomy was done for all patients; 45 patients (90%) by laparoscopic Cholecystectomy and 5 patients (10%) by open surgery and removed gallbladders were submitted to pathology department for detection of H. pylori in tissue under microscope using Giemsa stain. The results showed that (82%) were females with mean age (42.6+1years). The mean BMI was (29+7.2) H. pylori-specific antigen in stool samples was detected in 40% of patients and38% were detected in patients; tissue, with significant correlation between H. pylori-specific antigen in stool and in tissue. Histopathological pictures infection in tissue were 68.4% mucosal erosions, 63.2% mucosal atrophy, 57.9% mucosal hyperplasia, 26.3% metaplasia, 42.1% musculosa hypertrophy, 26.3% fibrosis, but lymphoid aggregates were in 42.1% of cases.

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Evaluation of Salivary and Serum Anti-Helicobacter Pylori in Egyptian Patients with H. Pylori Related Gastric Disorders

Cited by 8 publications

References 19 publications

Comparison of the serum and salivary antibodies to detect gastric Helicobacter pylori infection in Kashan (Iran)

Comparison of the serum and salivary antibodies to detect gastric Helicobacter pylori infection in Kashan (Iran)

Oral and Gastric Helicobacter Pylori: Effects and Associations

Detection of Helicobacter Antigen in Stool Samples and Its Relation to H. Pylori Positive Cholecystitis in Egyptian Patients with Chronic Calcular Cholecystitis

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