2021
DOI: 10.1371/journal.pone.0247767
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Evaluation of sample pooling for screening of SARS CoV-2

Abstract: Background The coronavirus disease 2019 (COVID-19) pandemic has revealed the global public health importance of robust diagnostic testing. To overcome the challenge of nucleic acid (NA) extraction and testing kit availability, an efficient method is urgently needed. Objectives To establish an efficient, time and resource-saving and cost-effective methods, and to propose an ad hoc pooling approach for mass screening of SARS-CoV-2. Methods We evaluated pooling approach on both direct clinical and NA samples.… Show more

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Cited by 25 publications
(19 citation statements)
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“…Furthermore, the saliva stored in the RNA stabilization buffer and heat-treated can be used in nucleic acid amplification tests without the RNA extraction step, which considerably reduces the labor costs and shortens the time from sample collection to diagnosis. For mass testing of populations with low SARS-CoV-2 infection prevalence, sample pooling can be introduced in diagnostics to reduce resource consumption [28]. Although RT-LAMP is a less favorable method than RT-qPCR due to its reduced sensitivity, it is a suitable PoC method in which qPCR machines are not available.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Furthermore, the saliva stored in the RNA stabilization buffer and heat-treated can be used in nucleic acid amplification tests without the RNA extraction step, which considerably reduces the labor costs and shortens the time from sample collection to diagnosis. For mass testing of populations with low SARS-CoV-2 infection prevalence, sample pooling can be introduced in diagnostics to reduce resource consumption [28]. Although RT-LAMP is a less favorable method than RT-qPCR due to its reduced sensitivity, it is a suitable PoC method in which qPCR machines are not available.…”
Section: Discussionmentioning
confidence: 99%
“…If the prevalence of viral infection among the tested individuals is low, then sample pooling might be a suitable strategy to minimize the testing costs [28]. Generally, NPS or saliva in the first days of infection yields approximately 10 5 viral RNA molecules per milliliter [29,30].…”
Section: Sample Poolingmentioning
confidence: 99%
“…Pool testing is currently a suitable strategy for coping with the COVID-19 pandemic. More than ever, it is necessary to expand access to COVID-19 diagnostic tests and to identify presymptomatic and asymptomatic individuals who contribute silently to the fast dissemination and maintenance of the pandemic 15 . Increased testing has also become essential as a tool to reduce the rapid dissemination of the virus and an increase in mutant variants, which are a growing threat worldwide 16 .…”
Section: Introductionmentioning
confidence: 99%
“…Further, pooled testing reduces the effort for participants, making it easier to monitor over extended periods of time. Pooling has been reported for PCR tests detecting SARS-CoV-2 [ 13 , 14 , 15 , 16 ]. A recent report from a company suggests that pooled samples can successfully be analyzed by lateral flow point of care (PoC) test cassettes [ 17 ], but pooling has not become an established practice for rapid antigen tests.…”
Section: Introductionmentioning
confidence: 99%