Evaluation of Schistosoma bovis adult worm extract for vaccination of calves

Aradaib, Imadeldin E.; Abbas, B.; Bushara, H. O.; Taylor, M. G.

doi:10.1016/0167-5877(93)90077-7

Cited by 12 publications

(5 citation statements)

References 15 publications

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“…Schistosomiasis is one of the major veterinary problems in many Africa countries. Cattle infected with Schistosoma bovis develop a syndrome characterized by liver damage, roughness of hair coat, pale mucous membrane, serious emaciation and a very poor reproductive performance (Aradaib et al, 1993) that results in a significant economic down turn and a threat to public health. This study is aimed at determining the distribution of Schistosoma infection in cattle in Maiduguri Metropolis and Jere LGAs of Borno state.…”

Section: Introductionmentioning

confidence: 99%

Sero-prevalence of Schistosoma species in cattle in Maiduguri Metropolis and Jere Local Government Areas of Borno State, Nigeria

Hambali¹,

Adamu²,

Ahmed³

et al. 2016

J Adv Vet Anim Res

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Objectives: This study was designed to investigate the Sero-prevalence of Schistosoma species in cattle in Maiduguri Metropolis (MMC) and Jere Local Government Areas (LGAs) of Borno State, Nigeria. Materials and Method: Blood samples (n=200) from cattle were collected using a multistage sampling technique; 100 samples each were collected from MMC and Jere LGAs, respectively. The samples were subjected to screening for Schistosoma antibodies using Enzyme Linked Immunosorbent Assay (ELISA). Age, sex, breed and location of cattle were recorded. Results: The overall prevalence of Schistosoma infection among cattle in MMC and Jere LGAs was 10%. Jere LGA had a prevalence rate of 14% and MMC had 6%. At the ward levels, Custom Area in Jere LGA had the highest number of Schistosoma positive (50%). Out of 103 female and 97 male cattle screened, the prevalence of Schistosoma infection in female and male were 9.71% (n=10/103) and 10.31% (n=10/103). Out of the 177 serum samples from cattle aging >1-year (adult) examined, 16 (9.04%) were positive, while only 4 (17.39%) out of 23 serum samples from cattle aging <1-year (young) were positive. Out of the eight (8) breeds screened, the highest number of cases was recorded in Kuri breed (16.22%). This was followed by Sokoto Gudali (10.9%) breed. The prevalence in other breeds was as follows: Abore-10%, Red Bororo-5.26%, and White Fulani-6.67%. Conclusion: It is concluded that schistosomiasis in cattle was prevalent in MMC and Jere LGAs of Borno State. A regular checking program is suggested to constantly check out whether the prevalence rate is increasing, so that effective control measures can be strenthened.

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Section: Introductionmentioning

confidence: 99%

Sero-prevalence of Schistosoma species in cattle in Maiduguri Metropolis and Jere Local Government Areas of Borno State, Nigeria

Hambali¹,

Adamu²,

Ahmed³

et al. 2016

J Adv Vet Anim Res

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“…Variation between our microscopic and molecular results could because valuable sensitivities and specificities of molecular assays in addition to facts that cattle harboring Schistosoma could not be showed a positivity using traditional techniques as parasitic eggs may be trapped in various tissues and are not get a way to be excreted by feces [9,37]. On other hand, cattle of older ages might be acquired a strong specific immunity to suppress worm fecundities as well as releasing of eggs [2,38].…”

Section: Total Resultsmentioning

confidence: 92%

Molecular Detection of Schistosoma spp. in Cattle Urine in Mosul, Iraq

Gharban,

Ajaj

2024

Egyptian Journal of Veterinary Sciences

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T HIS STUDY conducts to identification the microscopic prevalence of schistosome infection in cattle urine and molecular detection of Schistosoma bovis in urine and blood. Totally, 70 anorexic, emaciated and intermittent diarrheic cattle of different ages and sexes were selected and subjected to collection of urine that sediment firstly and tested by light microscope and the samples blood that examined molecularly using the conventional polymerase chain reaction (PCR) assay. Our findings revealed that 11.43% of study animals were positives by microscopy. Targeting the 18S rRNA gene, the positive findings using the PCR assay detected that (21.43%) of in blood samples were positive; whereas, no positivity was seen in urine. Regarding age, results of microscopy were significantly elevated (P<0.0216) in cattle of >1 year (14.89%) when compared to those of <1 year (4.35%); while molecularly, insignificant variation (P<0.0939) was seen between values of <1 year (21.74%) and >1 year (21.28%). Concerning sex, the findings of microscopy and PCR reported that females (15.39% and 28.21%, respectively) having a higher positivity (P<0.05) than males (6.45% and 129%, respectively). In conclusion, this represents the first Iraqi study indicates traditionally the prevalence of bovine schistosomiasis in urine; and molecularly, S. bovis in blood. The speciesspecific DNA detection by PCR appears to be more sensitive than clinical and traditional techniques, in addition to its ability in providing a high valuable data about the prevalence of disease. Moreover studies are recommended be done in other Iraqi areas among other domestic animals to explain their role in transmission of infection.

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“…Precipitin lines were also observed with sera from calves experimentally infected or immunized with Schistosoma bovis adult worm extracts or whole-egg antigen [66]. Cross reactions in the AGID between whole-egg, cercarial or adult worm antigens were also observed [67]. No precipitin lines were obser ved with sera from calves experimentally immunized with irradiated Schistosoma bovis schistosomula [66].…”

Section: Agar-gel Immunodiffusion Test (Agid)mentioning

confidence: 91%

“…The method adopted was basically that described by Ouchterloney [62] with the modification of Aradaib et al [63] Precipitin lines were seen when S. bovis adult worm antigen was tested against hyperimmune será from Schistosoma bovis infected rabbit and mice [64,65]. Precipitin lines were also observed with sera from calves experimentally infected or immunized with Schistosoma bovis adult worm extracts or whole-egg antigen [66].…”

Section: Agar-gel Immunodiffusion Test (Agid)mentioning

confidence: 99%