Evaluation of Scopoletin from Penicillium janthinellum Snef1650 for the Control of Heterodera glycines in Soybean

Abstract

Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is responsible for causing a major soybean disease globally. The fungal strain Penicillium janthinellum Snef1650 was evaluated against H. glycines. However, the effective determinants of the P. janthinellum strain are unknown. By performing pot experiments, a functioning compound was isolated from P. janthinellum Snef1650 through organic solvent extraction, semi-preparative HPLC, Sephadex LH-20 column chromatography, and silica gel column chromatography, and the isolated compound was identified to be scopoletin through 1H NMR, 13C NMR, and HPLC–MS. The pot experiments indicated that the treatment of soybean seeds with scopoletin drastically reduced the SCN population. The field experiments performed in 2017 and 2018 revealed that scopoletin decreased over 43.7% juveniles in the roots and over 61.55% cysts in the soil. Scopoletin treatment also promoted soybean growth and improved its yield, with an increase in plot yield by >5.33%. Scopoletin obtained from P. janthinellum Snef1650 could be used as an anti-H. glycines biocontrol agent.

Biocontrol of Three Severe Diseases in Soybean

“…Scopoletin isolated from P. janthinellum Snef1650 was coated with soybean seeds and reduced the juveniles of H. glycines in the roots and cysts in the soil in the field assay. Meanwhile, scopoletin promoted the growth and yield of soybean plants [89]. Similarly, two P. oxalicum strains, NBC008 and NBC012, reduced the cyst number and improved the height and root length of soybean plants compared with the control [85].…”

“…Metabolite compounds from Streptomyces strains also exhibited remarkable effects against S. sclerotiorum [122]. Scopoletin was isolated from P. janthinellum Snef1650 and could decrease the number of cysts in the soil and juveniles in the soybean roots [89]. The L. enzymogenes C3 mutant was defective in antimicrobial secondary metabolite complex production and reduced the inhibitory effect on the reproduction of H. glycines [99].…”

Engineered coumarin accumulation reduces mycotoxin‐induced oxidative stress and disease susceptibility

“…Yet, to our knowledge, there is no report on the correlation between nematode infection and the accumulation of coumarins in plants. However, recent studies revealed scopoletin to be a potent biocontrol agent against SCN, with scopoletin being active after the treatment of soybean seeds with the compound (Yan et al ., 2021). SCNs are in the same genus as H. schachtii and form a disease complex with F. virguliforme , thereby leading to stronger SDS severity of soybean (Westphal et al ., 2014; Xing and Westphal, 2006).…”

Enhanced Bioactivity of Botanical Acaricidal Compound-Scopoletin Encapsulated by Polydopamine and Cellulose Nanocrystals Against Carmine Spider Mite

“…19 In agricultural applications, Sco presents insecticidal activity, antibacterial sterilization action, nematicidal bioactivity, and allelopathy. 20,21 In our previous study, we found that Sco extracted from Artemisia annua exhibits strong acaricidal activity against CSM. 11 However, Sco is difficult to use commercially because it has poor water solubility and is strongly susceptible to photolysis.…”