Evaluation of ADAM‐rWBC for counting residual leucocytes in leucocyte‐reduced whole blood and apheresis platelet concentrates

Methods for counting residual leukocytes in leukocyte‐depleted plasma—a comparison between a routine hematology instrument, the Nageotte chamber, flow cytometry, and a fluorescent microscopy analyzer

“…The ADAM‐rWBC has previously been found suitable for measuring residual WBCs in platelet concentrates and in whole blood . In the study by Strobel and coworkers, the results from ADAM‐rWBC and flow cytometry analyses were comparable, whereas the Nageotte chamber produced results with a negative bias and had lower accuracy.…”

“…Polymerase chain reaction methods have also been suggested but were regarded expensive and even more laborious than the Nageotte chamber method . Recently, a dedicated fluorescent microscopy analyzer was introduced with promising results, but the instrument had previously been tested only on platelet concentrates and in leukocyte‐depleted whole blood …”

Leukoreduction filters: Still stuck on sickle trait red cells

“…A gold standard for rWBC enumeration does not exist, and most U.S. blood centers utilize automated flow cytometry or the ADAM‐rWBC (NanoEntek, Waltham, MA, USA), a portable semiautomated fluorescence analyzer that is less costly than flow cytometry and requires less staff training to operate 31,32 . Specifications for rWBC testing utilizing the ADAM‐rWBC demonstrate a measuring range from 1 to 100 cells/μl, with ≥80% accuracy and a ≤20% coefficient of variation at rWBC concentrations down to 3 cells/μl 34 . This range encompasses the 15–20 cells/μl representing a rWBC content of 5 × 10 6 in most RBC units, but is incapable of quantitating counts >3 × 10 7 /unit.…”

“…31,32 Specifications for rWBC testing utilizing the ADAM-rWBC demonstrate a measuring range from 1 to 100 cells/μl, with ≥80% accuracy and a ≤20% coefficient of variation at rWBC concentrations down to 3 cells/μl. 34 This range encompasses the 15-20 cells/μl representing a rWBC content of 5 Â 10 6 in most RBC units, but is incapable of quantitating counts >3 Â 10 7 /unit. In this issue, Gehrie et al present rWBC quality control data on 40 WB collections that had successfully completed cold filtration (1 WB-and 39 RBC-filtered products) from donors with historically positive HbS screening test results.…”

An evaluation of a volumetric method for the flow cytometric determination of residual leukocytes in blood transfusion units

“…Therefore, the residual leukocyte count in a small sample volume is determined with other methodologies, including microscopy and flow cytometry. The manual microscopy with a Nageotte chamber, which was one of the first residual leukocyte counting methods, or a portable fluorescent microscopic leukocyte cell counter ADAM-rWBC (NanoEntek), are two well-known non-flow cytometric counting procedures [10][11][12]. As both have their limitations, the Nageotte hemocytometer is less accurate and less precise and is time-consuming, the ADAM-rWBC shows better results for low-level leukocyte enumeration in blood samples, but still has a narrow scope [13,14], flow cytometry is a much more flexible technique and can achieve counts even when there are very low cell numbers.…”