Evaluation of Screening Methods for the Isolation of Biosurfactant Producing Marine Bacteria

Thavasi, Rengathavasi; Sharma, Shilpy; Jayalakshmi, S.

doi:10.4172/2157-7463.s1-001

Cited by 77 publications

(73 citation statements)

References 43 publications

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“…bacteria, archaea and fungi) are isolated from environmental samples, such as soil and water, collected from around the world. [23,29,32,33] A small percentage of environmental isolates normally produce BS. [33] Interestingly, this number can rise to 25% in samples from hydrocarbon or heavy metals-polluted sites or even more dramatically (up to 80%) when enrichment cultures with hydrocarbons are used.…”

Section: Isolation and Screening Of Bs-producing Microorganismsmentioning

confidence: 99%

“…[8,11,20,21] The third branch of BS research is searching for novel molecules. [22][23][24][25] The identification of novel LP is critical to enrich our knowledge of BS and will provide researchers with molecules that may have desirable characteristics, such as high temperature stability, activity in a broad range of pH and salinity levels, satisfactory biodegradability and/ or new biological activities. [2,[26][27][28] Although many valuable papers on BS have been published, we found no concise review that introduced and described a workflow of LP/BS research.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Screening concepts, characterization and structural analysis of microbial-derived bioactive lipopeptides: a review

Biniarz

Łukaszewicz

Janek

2016

Critical Reviews in Biotechnology

103

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Lipopeptide biosurfactants are surface active biomolecules that are produced by a variety of microorganisms. Microbial lipopeptides have gained the interest of microbiologists, chemists and biochemists for their high biodiversity as well as efficient action, low toxicity and good biodegradability in comparison to synthetic counterparts. In this report, we review methods for the production, isolation and screening, purification and structural characterization of microbial lipopeptides. Several techniques are currently available for each step, and we describe the most commonly utilized and recently developed techniques in this review. Investigations on lipopeptide biosurfactants in natural products require efficient isolation techniques for the characterization and evaluation of chemical and biological properties. A combination of chromatographic and spectroscopic techniques offer opportunities for a better characterization of lipopeptide structures, which in turn can lead to the application of lipopeptides in food, pharmaceutical, cosmetics, agricultural and bioremediation industries. ARTICLE HISTORY

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Section: Isolation and Screening Of Bs-producing Microorganismsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Screening concepts, characterization and structural analysis of microbial-derived bioactive lipopeptides: a review

Biniarz

Łukaszewicz

Janek

2016

Critical Reviews in Biotechnology

103

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“…Thavasi et al [18] obtained surface tension values ranging between 30.8 -38.75 mN/m for potential biosurfactant producers among Bacillus species. Surface tension value above 45 mN/m is regarded as negative for biosurfactant production [22].…”

Section: Resultsmentioning

confidence: 99%

“…Of five isolates positive for oil spreading technique, it was found that isolate 4M5 produced the largest diameter of clearing zone of more than 1.5 cm, suggesting high biosurfactant concentration in the culture medium. Thavasi et al [18] showed potential biosurfactant producers produced diameter of clearing zone of ≥0.5 cm. Karthik et al [19], employing oil spreading technique as one of their screening methods, reported a potential biosurfactant-producing microorganism produced diameter of clearing zone in the range of 0.9 -2.0 cm, whereas poor biosurfactant producers produced 0.1 -0.2 cm.…”

Section: Resultsmentioning

confidence: 99%

DETERMINATION OF OPTIMUM CONDITIONS AND STABILITY STUDY OF BIOSURFACTANT PRODUCED BY Bacillus subtilis UKMP-4M5

Hamzah¹

2016

MJAS

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Bacillus subtilis UKMP-4M5 was isolated from a hydrocarbon contaminated site which was found to be the most potential biosurfactant producer. Isolates were grown in a mineral salt medium (MSM) supplemented with sunflower oil (1% v/v) as the main carbon source. Screening was based on both qualitative (oil spreading technique) and quantitative (emulsification index and surface tension measurement) methods. B. subtilis UKMP-4M5 produced the highest oil displacement activity with and emulsification index of 17%. It also reduced surface tension of culture medium from 58.95 to 41.75 mN/m. B. subtilis UKMP-4M5 produced biosurfactant with surface tension measurement of 32.7 ± 0.66 mN/m using 2% (v/v) palm oil and 0.5% (w/v) yeast extract as carbon and nitrogen sources respectively. The tested biosurfactant exhibited excellent thermal (up to 120 °C) and pH (6.0 -8.0) stability as well as high tolerance for varying salt concentrations (1 -5% w/v) in terms of surface tension reducing ability. This suggests potential applications in fields such as enhanced oil recovery, bioremediation, and the food industry. Keywords: Bacillus subtilis, biosurfactant, palm oil, surface tension AbstrakBacillus subtilis UKMP-4M5 yang dipencilkan dari kawasan yang tercemar dengan hidrokarbon didapati paling berpotensi sebagai penghasil biosurfaktan. Pencilan dihidupkan di dalam medium garam mineral (MSM) yang ditambahkan dengan 1% (i/i) minyak bunga matahari sebagai sumber karbon utama. Penyaringan adalah berdasarkan kepada kedua-dua kaedah kualitatif (teknik penyebaran minyak) dan kuantitatif (indeks emulsifikasi dan ukuran ketegangan permukaan). B. subtilis UKMP-4M5 merekodkan aktiviti sesaran minyak yang tertinggi dengan indeks emulsifikasi sebanyak 17%. Ia juga merendahkan ketegangan permukaan kultur medium dari 58.95 mN/m ke 41.75 mN/m B. subtilis UKMP-4M5 menghasilkan biosurfaktan dengan ukuran ketegangan permukaan 32.7 ± 0.66 mN/m menggunakan 2% (i/i) minyak kelapa sawit dan 0.5% (b/i) ekstrak yis masing-masing sebagai sumber karbon dan nitrogen. Biosurfaktan yang diuji menunjukkan kestabilan yang amat baik pada suhu (sehingga 120 °C) dan pH (6.0 -8.0) serta toleransi yang tinggi pada kepekatan garam yang berbeza (1 -5% b/i) dari segi keupayaannya merendahkan ketegangan permukaan. Ini mencadangkan potensi aplikasinya dalam industri seperti peningkatan perolehan minyak, bioremediasi dan industri makanan.

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“…According to Thavasi et al (2011), microorganisms with positive hemolytic activity for production of biosurfactants show a clear zone in the blood agar plates. In this context, only the strain Rhizopus spp.…”

Section: Detection Of Biosurfactant-producing Rhizopus Strainsmentioning

confidence: 99%

Development and improved selected markers to biosurfactant and bioemulsifier production by Rhizopus strains isolated from Caatinga soil

Pele¹,

Rodríguez²,

Ribeaux³

et al. 2018

Afr. J. Biotechnol.

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This study screened four Rhizopus species as biosurfactant producers using different markers. First of all, Rhizopus spp. UCP 1607 was identified as Rhizopus arrhizus by morphological and molecular methods. The production of biosurfactant/bioemulsifier was investigated by submerged fermentation using soybean post-frying oil (5% v/v) and sodium glutamate (1% w/v) medium. The primary markers' hemolysis and parafilm M tests showed that R. arrhizus UCP1607 strain exhibited higher hemolytic activity (49 mm of clear zone) on sheep blood agar and a larger drop diameter (12 mm) on parafilm hydrophobic surface. The experimental results showed the most promising biosurfactant production by R. arrhizus UCP 1607 strain led to a reduction of surface tension (31.8 mN/m) and the diameter of the oil-spreading covered an area of 66.4 cm 2 . The strains Rhizopus microsporus var. chinensis UCP1296, R. microsporus var. microsporus UCP1304, and R. arrhizus UCP1607 were capable of forming stable emulsions corresponding to 91.7, 94.8, and 82.6%, respectively in crude oil.

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Evaluation of Screening Methods for the Isolation of Biosurfactant Producing Marine Bacteria

Cited by 77 publications

References 43 publications

Screening concepts, characterization and structural analysis of microbial-derived bioactive lipopeptides: a review

Screening concepts, characterization and structural analysis of microbial-derived bioactive lipopeptides: a review

DETERMINATION OF OPTIMUM CONDITIONS AND STABILITY STUDY OF BIOSURFACTANT PRODUCED BY Bacillus subtilis UKMP-4M5

Development and improved selected markers to biosurfactant and bioemulsifier production by Rhizopus strains isolated from Caatinga soil

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