Resin-based pit and fissure sealant materials are heterogeneous materials with two principal components: the resin matrix and the filler particles. The shear bond strength test holds great importance for providing insight into the adhesion of these materials and, also, it is a screening mechanism for predicting clinical performance. The purposes of the present study are to investigate the structure of surface and the shear bond strength for two commercial pit and fissure sealants, Fissurit (VOCO) and Pit and Fissure (DMP) sealer. Seal Fissurit had the highest shear strength, and the difference was statistically significant between two tested materials.