The study aimed to evaluate the effectiveness and challenges of port health services. Utilizing a sample size of 134 respondents, the study assessed key indicators including awareness of port health services, attitudes towards their importance and effectiveness, perceptions of service quality, interactions with other regulatory authorities, and challenges and opportunities for improvement. Results revealed a high level of awareness regarding health services and protocols, with generally positive attitudes towards their importance and effectiveness. However, concerns were noted about the competence of health officials and trust in implemented measures. Significant challenges, such as resource constraints and infrastructural issues, were identified, along with substantial opportunities for enhancing service delivery through technological upgrades and improved staff training. Recommendations emphasize the need for better collaboration among regulatory bodies, investment in modern infrastructure, and increased stakeholder awareness to enhance the efficiency and effectiveness of port health services at Rusumo OSBP.