Evaluation of Selective and Nonselective Media for Isolation of Helicobacter pylori from Gastric Biopsy Specimens

Alikhani, M Y; Sadeghifard, Sepehr; Farajnia, S; Hajia, Masood; Aslani, Mohammad Mehdi; Zamani, Ali

doi:10.3923/pjbs.2007.4156.4159

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…The best medium for the primary isolation of H. pylori was the selective Pylori agar, which is more suitable for its detection mainly because it is more likely to avoid the transformation of vital germs into coccoid forms that are unable to grow and which would thus not be suitable for antibiotic sensitivity study. 36,37 The low percentage of H. pylori isolated in our study (56%), considering that all our patients were infected because they persistently resulted positive to both Urea Breath test and histological examinations, can also be due to the fact that they could yield only very low numbers of bacteria (too low to be cultured) owing to several previous treatments or to the presence of metabolic inactive microorganisms that are insensible to antibiotics.…”

Section: Discussionmentioning

confidence: 79%

“…First of all, in the present study, the isolation rate was likely influenced by the sole use of the culture technique. By overcoming the difficulty in accessing the H. pylori ecological niche and the fragile nature of the bacterium, 36 non-culture methods (such as fluorescence in situ hybridization -FISH test) may be more sensitive than culture-based techniques. 33 Since a perfect method for H. pylori isolation is not available and the methods used in each laboratory strongly affect its detection, two growing media were used and compared.…”

Section: Discussionmentioning

confidence: 99%

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Impact of Helicobacter pylori resistance in unsuccessfully pluritreated patients in a Department of Infectious Diseases in Rome

Mascellino

Porowska

Nicosia³

et al. 2010

Microbiol Res (Pavia)

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Twenty-five pluritreated patients were examined. Fifty-six percent yielded Helicobacter pylori (H. Pilory); of these, 9 patients showed a concomitant colonization of the three gastric regions. The highest resistance rate was found for metronidazole (71.8%) followed by chlaritromycin (53.1%). Amoxycillin showed the best susceptibility (only 6% of resistance), tetracycline showed 12% of resistant strains and levofloxacin appeared to be a promising antibacterial agent (18% of resistance). The E-test method was shown to be more suitable than disk diffusion technique for resistance testing. Combined resistance to both chlaritromycin and metronidazole appeared in 50% of the strains. The isolates showing this dual resistance are known to be difficult to eradicate. Resistotypes were shown to be genotypically different even if the strains with the resistance to both chlaritromycin and metronidazole are more likely to belong to genotype cagA+ and vacA s1m1. Heteroresistance (different susceptibility of the isolated strains in a single stomach) resulted in 36% of patients with pangastritis. Indeed, the concomitant presence of H. pylori strains in the same subject, either susceptible or resistant or vice versa, may interfere with the eradication outcomes. In our study, antibiotic resistant H. pylori typically develops from pre-existing susceptible strains rather than from co-infection with a different and unrelated strain. In fact, each pair of isolates detected in our 4 patients with heteroresistance belonged to the same genotype (cagA+ s1m2 in patient 1 and cagA+ s1m1 in patients 2, 3 and 4). In conclusion, H. pylori antibiotic resistance does present several issues in pluritreated patients owing to the rapid emergence of multi-resistant strains

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Impact of Helicobacter pylori resistance in unsuccessfully pluritreated patients in a Department of Infectious Diseases in Rome

Mascellino

Porowska

Nicosia³

et al. 2010

Microbiol Res (Pavia)

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Helicobacter pylori vacA virulence factor in uncultured Helicobacter heilmannii sensu lato from an infected child

et al. 2016

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Introduction: Helicobacter spp. colonizing the human stomach other than Helicobacter pylori have been rarely described in children. Case Presentation: We identified an uncultured Helicobacter heilmannii sensu lato by histology and PCR in biopsies from a symptomatic child. Surprisingly, the PCR assay demonstrated the presence of the vacA gene in this uncultured H. heilmannii sensu lato and its sequence related highly to H. pylori vacA virulence factor. The analysis of the 16S ribosomal sequence showed higher identity with uncultured Helicobacter spp. and the H. heilmannii strain, respectively. Conclusion: This is the first report that shows the presence of vacA in an uncultured H. heilmannii sensu lato extracted from human gastric mucosa.

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Consequences of Chronic Bacterial Infections on Development of Cancer

Ibrahim¹,

Makkiya²

2011

Research J. of Microbiology

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Evaluation of Selective and Nonselective Media for Isolation of Helicobacter pylori from Gastric Biopsy Specimens

Cited by 3 publications

References 24 publications

Impact of Helicobacter pylori resistance in unsuccessfully pluritreated patients in a Department of Infectious Diseases in Rome

Impact of Helicobacter pylori resistance in unsuccessfully pluritreated patients in a Department of Infectious Diseases in Rome

Helicobacter pylori vacA virulence factor in uncultured Helicobacter heilmannii sensu lato from an infected child

Consequences of Chronic Bacterial Infections on Development of Cancer

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