Evaluation of sensitivity, specificity and cross-reactivity in Bartonella henselae serology

Vermeulen, Marijn J.; Verbakel, Harold; Notermans, Daan W.; Reimerink, Johan; Peeters, Marcel

doi:10.1099/jmm.0.015248-0

Cited by 75 publications

(65 citation statements)

References 12 publications

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“…12,13 In addition, due to the lack of Bartonella species-specific antibody response, crossreactivity between different Bartonella spp., Epstein-Barr virus, cytomegalovirus, Toxoplasma gondii and Streptococcus pyogenes may occur. 12,14,15 Concerning histopathological examination of affected lymph nodes to determine the cause of lymphadenopathy, there is no specific pathology suggestive for B. henselae infection. 12 Even when Warthin-Starry silver stain is used to identify the causative bacterium, histopathologic findings are strongly suggestive for CSD but not definitive.…”

Section: Discussionmentioning

confidence: 99%

Seronegative cat-scratch disease diagnosed by PCR detection of Bartonella henselae DNA in lymph node samples

Chondrogiannis¹,

Vezakis²,

Derpapas³

et al. 2012

Braz J Infect Dis

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Section: Discussionmentioning

confidence: 99%

Seronegative cat-scratch disease diagnosed by PCR detection of Bartonella henselae DNA in lymph node samples

Chondrogiannis¹,

Vezakis²,

Derpapas³

et al. 2012

Braz J Infect Dis

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“…The presence of IgM in patients is clear evidence of recent contact with B. henselae; however, the sensitivity of assays in the detection of the IgM is low (5)(6)(7)(8)(9)(10)12). IgM remains in the serum for approximately 3 months (7,13).…”

Section: Discussionmentioning

confidence: 99%

“…We proved that the ELISAs using antigen II, IV, and V could clearly distinguish between the two sets of sera, whereas the ELISAs with antigen I and III showed overlapping results for the two sets, indicating that these two antigens were not suitable for use in the IgM-ELISA. There have been many previous reports on the development of IgM-ELISAs using whole-cell protein (antigen I) (6)(7)(8)(9)(10), which have generally shown poor sensitivity. We confirmed that whole-cell protein was inappropriate for use in the IgM-ELISA.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Development of a Highly Specific IgM Enzyme-Linked Immunosorbent Assay for Bartonella henselae Using Refined N -Lauroyl-Sarcosine-Insoluble Proteins for Serodiagnosis of Cat Scratch Disease

et al. 2016

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The conventional anti-Bartonella henselae IgM enzyme-linked immunosorbent assay (IgM-ELISA) methods for diagnosing cat scratch disease (CSD) remain poor in both sensitivity and specificity. We sought to develop an IgM-ELISA with improved accuracy in the serodiagnosis of CSD by exploring the antigens that are most suitable for an ELISA. We prepared 5 different protein antigens: antigen I (sonicated B. henselae whole-cell antigen), antigen II (N-lauroyl-sarcosine-insoluble antigen), antigen III (processed sarcosine-soluble antigen), and antigen IV and antigen V (sarcosine-insoluble and sarcosine-soluble antigens refined by DEAE-Sepharose Fast Flow ion-exchange chromatography). The IgM antibodies in the sera of 47 patients with clinically suspected CSD (24 definite, 23 suspected) and of 85 healthy individuals were examined by ELISAs using the 5 antigens, and the results were compared with those of an IgM indirect fluorescent antibody assay (IgM-IFA). In a reference panel, which consisted of 5 positive and 5 negative sera, antigen I and antigen III failed to distinguish between the two statuses, whereas the other three antigens succeeded in distinguishing between them. When the cutoff value was set at the 98th percentile of the ELISA index for healthy individuals, the sensitivity of IgM-IFA for the 24 cases of definite CSD was 54%, whereas the sensitivities of the IgM-ELISAs with antigen II, IV, and V were 75%, 83%, and 75%, respectively. The sensitivities of these three IgM-ELISAs for all 47 of the clinically suspected cases were 49%, 64%, and 51%, respectively. In contrast, the sensitivity of IgM-IFA was 28%. These results indicate that the refined sarcosine-insoluble proteins (antigen IV), which possessed the highest specificity among the 5 antigens, are the most appropriate for developing an IgM-ELISA for the highly specific serodiagnosis of CSD.

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“…and Streptoccocus spp. is rare when samples are evaluated with this serological assay (Sykes et al 2006, Vermeulen et al 2010). However, a potential limitation of our findings is that we cannot rule out the possibility of cross-reactive antibodies to other intracellular pathogens that have shown to interfere in the diagnosis of bartonellosis in dogs and humans (Maurin et al 2002, MacDonald et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Bartonellaspp. Exposure in Northern and Southern Sea Otters in Alaska and California

Carrasco

Chomel

Gill

et al. 2014

Vector-Borne and Zoonotic Diseases

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Since 2002, an increased number of northern sea otters (Enhydra lutris kenyoni) from southcentral Alaska have been reported to be dying due to endocarditis and/or septicemia with infection by Streptococcus infantarius subsp. coli. Bartonella spp. DNA was also detected in northern sea otters as part of mortality investigations during this unusual mortality event (UME) in Kachemak Bay, Alaska. To evaluate the extent of exposure to Bartonella spp. in sea otters, sera collected from necropsied and live-captured northern sea otters, as well as necropsied southern sea otters (Enhydra lutris nereis) unaffected by the UME, were analyzed using an immunofluorescent antibody assay. Antibodies against Bartonella spp. were detected in sera from 50% of necropsied and 34% of presumed healthy, live-captured northern sea otters and in 16% of necropsied southern sea otters. The majority of sea otters with reactive sera were seropositive for B. washoensis, with antibody titers ranging from 1:64 to 1:256. Bartonella spp. antibodies were especially common in adult northern sea otters, both free-living (49%) and necropsied (62%). Adult stranded northern sea otters that died from infectious causes, such as opportunistic bacterial infections, were 27 times more likely to be Bartonella seropositive than adult stranded northern sea otters that died from noninfectious causes ( p < 0.001; 95% confidence interval 2.62-269.4). Because Bartonella spp. antibodies were detected in necropsied northern sea otters from southcentral (44%) and southwestern (86%) stocks of Alaska, as well as in necropsied southern sea otters (16%) in southcentral California, we concluded that Bartonella spp. exposure is widely distributed among sea otter populations in the Eastern Pacific, providing context for investigating future disease outbreaks and monitoring of Bartonella infections for sea otter management and conservation.

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Evaluation of sensitivity, specificity and cross-reactivity in Bartonella henselae serology

Cited by 75 publications

References 12 publications

Seronegative cat-scratch disease diagnosed by PCR detection of Bartonella henselae DNA in lymph node samples

Seronegative cat-scratch disease diagnosed by PCR detection of Bartonella henselae DNA in lymph node samples

Development of a Highly Specific IgM Enzyme-Linked Immunosorbent Assay for Bartonella henselae Using Refined N -Lauroyl-Sarcosine-Insoluble Proteins for Serodiagnosis of Cat Scratch Disease

Bartonellaspp. Exposure in Northern and Southern Sea Otters in Alaska and California

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