Evaluation of serological diagnostic tests for bovine brucellosis in dairy cattle herds in an endemic area: a multicenter study

Alamian, Saeed; Amiry, Karim; Bahreinipour, Akram; Etemadi, Afshar; Yousefi, Ali Reza; Dadar, Maryam

doi:10.1007/s11250-023-03519-1

Cited by 2 publications

(1 citation statement)

References 38 publications

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“…In any case, certainly control of bovine brucellosis should led to zero cases in most territories, and eventually to elimination and even eradication of those territories. Nevertheless, serological tests are the most used and important diagnostic tools for the massive screening and control programs of brucellosis, as has been stated in different studies [ 77 ].…”

Section: Discussionmentioning

confidence: 99%

A systematic review and meta-analysis of bovine brucellosis seroprevalence in Latin America and the Caribbean

Bonilla-Aldana,

Trejos-Mendoza,

Pérez-Vargas

et al. 2023

New Microbes and New Infections

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Section: Discussionmentioning

confidence: 99%

A systematic review and meta-analysis of bovine brucellosis seroprevalence in Latin America and the Caribbean

Bonilla-Aldana,

Trejos-Mendoza,

Pérez-Vargas

et al. 2023

New Microbes and New Infections

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Investigation of the Seroprevalence of Brucella Antibodies and Characterization of Field Strains in Immunized Dairy Cows by B. abortus A19

Shi,

Cui,

Wudong

et al. 2024

Veterinary Sciences

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(1) Background: One method of eradicating brucellosis is to cull cattle that test positive for antibodies 12 months after being vaccinated with the 19-strain vaccine. Variations in immunization regimens and feeding practices may contribute to differences in the rate of persistent antibodies. We conducted this study to investigate the real positive rate of Brucella antibody in field strains of Brucella spp. after immunization over 12 months in dairy cows. This research aims to provide data to support the development of strategies for preventing, controlling, and eradicating brucellosis. (2) Method: We employed the baseline sampling method to collect samples from cows immunized with the A19 vaccine for over 12 months in Lingwu City from 2021 to 2023. Serological detection was conducted using the RBPT method. An established PCR method that could distinguish between 19 and non-19 strains of Brucella was utilized to investigate the field strains of Brucella on 10 dairy farms based on six samples mixed into one using the Mathematical Expectation strategy. (3) Results: We analyzed the rates of individual seropositivity and herd seropositive rates in dairy cattle in Lingwu City from 2021 to 2023 and revealed that antibodies induced by the Brucella abortus strain A19 vaccine persist in dairy herds for more than 12 months. We established a PCR method for identifying both Brucella A19 and non-A19 strains, resulting in the detection of 10 field strains of Brucella abortus from 1537 dairy cows. By employing a Mathematical Expectation strategy, we completed testing of 1537 samples after conducting only 306 tests, thereby reducing the workload by 80.1%. (4) Conclusions: There was a certain proportion of cows with a persistent antibody titer, but there was no evidence that all of these cattle were naturally infected with Brucella. The established PCR method for distinguishing between Brucella abortus strain 19 and non-19 strains can be specifically utilized for detecting natural Brucella infection in immunized cattle. We propose that relying solely on the detection of antibodies in cattle immunized with the A19 vaccine more than 12 months previously should not be solely relied upon as a diagnostic basis for brucellosis, and it is essential to complement this approach with PCR analysis to specifically identify field Brucella spp. Brucella abortus was the predominant strain identified in the field during this study. Detection based on the Mathematical Expectation strategy can significantly enhance detection efficiency.

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Evaluation of serological diagnostic tests for bovine brucellosis in dairy cattle herds in an endemic area: a multicenter study

Cited by 2 publications

References 38 publications

A systematic review and meta-analysis of bovine brucellosis seroprevalence in Latin America and the Caribbean

A systematic review and meta-analysis of bovine brucellosis seroprevalence in Latin America and the Caribbean

Investigation of the Seroprevalence of Brucella Antibodies and Characterization of Field Strains in Immunized Dairy Cows by B. abortus A19

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