Evaluation of Serum Adenosine Deaminase Changes Before and After Treatment in Patients with Systemic Lupus Erythematosus, Henoch-Schonlein Purpura and Juvenile Idiopathic Arthritis

Ziaee, Vahid; Amiran, Ali; Moradinejad, Mohammad-Hassan; Haghi-Ashtiani, Mohammad-Taghi

doi:10.5455/apr.021820131558

Cited by 5 publications

(4 citation statements)

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“…Due to this fact, it has been used as a marker of cell-mediated immunity [ 15 , 16 ] and chronic inflammation [ 17 ]. Several diseases have been reported to increase serum ADA activity in humans, including inflammation such as chronic tonsillitis, rhinosinusitis or otitis media [ 4 ], immune mediated disorders such as systemic lupus erythematosus [ 18 , 19 ] or rheumatoid arthritis [ 20 ], and malignancies such as chronic lymphocytic leukemia [ 21 ], breast cancer [ 22 ] or bladder cancer [ 23 ]. However, the response of both isoenzymes could be different as observed in some diseases, such as rheumatoid arthritis, in which ADA2 increases, whereas ADA1 does not [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of total adenosine deaminase activity (ADA) and its isoenzymes in saliva and serum in health and inflammatory conditions in four different species: an analytical and clinical validation pilot study

et al. 2020

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Background Measurement of adenosine deaminase (ADA) can provide information about cell-mediated immunity. This report’s objective was to study the enzymatic activity of total ADA (tADA) and its isoenzymes ADA1 and ADA2 in canine, equine, porcine, and bovine serum and saliva and their changes in different inflammatory situations in each species. Besides, an automated method for ADA2 measurement was developed and validated. Results tADA was present in serum and saliva of healthy animals of the four species. Erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) concentration of 0.47 mM was needed for ADA1 inhibition in canine and porcine samples (serum and saliva) and bovine saliva, whereas for equine saliva 0.94 mM was needed. ADA2 activity was not detected in bovine serum and was very low or absent in equine serum and bovine saliva. An automated procedure to measure ADA2 consisting of adding EHNA to a commercial reagent for tADA measurement provided repetitive (coefficients of variation < 8.8% in serum and < 10% in saliva) and accurate (linearity of serial sample dilutions with R2 > 0.90) results, being equivalent to a manual incubation of the sample with EHNA at a similar concentration. Salivary tADA, as well as ADA1 and ADA2, were higher in dogs with leishmaniosis, horses with acute abdominal disease and pigs with lameness than in healthy animals. tADA and isoenzymes in saliva showed a positive significant correlation with serum ferritin in dogs (r = 0.602, P < 0.01; r = 0.555, P < 0.05; and r = 0.632, P < 0.01; respectively for tADA, ADA1 and ADA2) and serum C-reactive protein in pigs (r = 0.700, P < 0.01, for both tADA and ADA1; r = 0.770, P < 0.001, for ADA2), whereas salivary ADA2 significantly correlated with serum amyloid A in horses (r = 0.649, P < 0.01). In cows, salivary tADA and ADA1 significantly increased after calving, correlating with total white blood cell count (r = 0.487, P < 0.05, for both tADA and ADA1). Conclusions The activity of total ADA and its different isoenzymes, can be measured in serum and saliva of dogs, horses, pigs and cows by a simple and fast procedure described in this report. When measured in saliva, these analytes correlated with other biomarkers of inflammation and it could potentially be used as a biomarkers of inflammation and immune activation in the species of this study.

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Section: Introductionmentioning

confidence: 99%

Characterization of total adenosine deaminase activity (ADA) and its isoenzymes in saliva and serum in health and inflammatory conditions in four different species: an analytical and clinical validation pilot study

et al. 2020

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“…Due to this fact, it has been used as a marker of cell-mediated immunity [15,16] and in ammation [17]. Several diseases have been reported to increase serum ADA activity in humans, including in ammation such as chronic tonsillitis, rhinosinusitis or otitis media [4], immune mediated disorders such as systemic lupus erythematosus [18,19] or rheumatoid arthritis [20], and malignancies such as chronic lymphocytic leukemia [21], breast cancer [22] or bladder cancer [23]. However, the response of both isoenzymes could be different since some diseases such as rheumatoid arthritis ADA2 increase due to chronic in ammation, whereas ADA1 does not increase [20].…”

mentioning

confidence: 99%

Characterization of total adenosine deaminase activity (ADA) and its isoenzymes in saliva and serum in health and inflammatory conditions to four different species: an analytical and clinical validation pilot study

Contreras-Aguilar

Tvarijonaviciute

Monkevičienė

et al. 2020

Preprint

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Background: Measurement of adenosine deaminase (ADA) can provide information about cell-mediated immunity. This report’s objetive was to study the enzymatic activity of total ADA (tADA) and its isoenzymes ADA1 and ADA2 in canine, equine, porcine, and bovine serum and saliva and their changes in different inflammatory situations in each species. Besides, an automated method for ADA2 measurement was developed and validated.Results: tADA was present in serum and saliva of healthy animals of the four species. Erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) concentration of 0.47mM was needed for ADA1 inhibition in canine and porcine samples (serum and saliva) and bovine saliva, whereas for equine saliva 0.94mM was needed. ADA2 activity was not detected in bovine serum and was very low or absent in equine serum and bovine saliva. An automated procedure to measure ADA2 consisting of adding EHNA to a commercial reagent for tADA measurement provided repetitive (coefficients of variation <8.8% in serum and <10% in saliva) and accurate (linearity under dilution approaches gave R 2 >0.90) results, being equivalent to a manual incubation of the sample with EHNA at a similar concentration. Salivary tADA, as well as ADA1 and ADA2, were higher in dogs with leishmaniosis, horses with acute abdominal disease and pigs with lameness than in healthy animals. ADA showed significant correlation with serum ferritin in dogs ( r = 0.602, P <0.01; r = 0.555, P <0.05; and r = 0.632, P <0.01; respectively for tADA, ADA1 and ADA2) and serum C-reactive protein in pigs ( r = 0.700, P <0.01, for both tADA and ADA1; r = 0.770, P <0.001, for ADA2), whereas salivary ADA2 significantly correlated with serum amyloid A in the horse ( r = 0.649, P <0.01). In the cow, salivary tADA and ADA1 significantly increased after calving, correlatingd with total white blood cell count ( r = 0.487, P <0.05, both).Conclusions: ADA activity, as well as its isoenzymes, can be measured in serum and saliva of different animal species. The salivary levels of these analytes could potentially be inflammatory biomarkers in these species.

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“…Also, serum ADA is widely used to differentiate between various infectious and malignant conditions in body fluids (23)(24)(25). Moreover, ADA has been suggested as an alternative parameter representing disease activity in systemic lupus erythematous, Juvenile Idiopathic arthritis, and Behcet's Disease (18,20,26). Synovial fluid ADA can also differentiate between RA and non-inflammatory arthritis (27).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Adenosine Deaminase Level in Serum and Synovial Fluid in Patients with Juvenile Idiopathic Arthritis and Its Relation to Inflammatory Acute Phase Reactants

Rahmani¹,

Raeeskarami²,

Ziaee³

et al. 2017

Iran J Pediatr

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Background: In JIA, cell-mediated immune response results in secretion of different inflammatory products from activated lymphocytes, macrophages, fibroblasts and leukocytes in synovial joints. Adenosine deaminase (ADA) regulates this immune system activity by metabolizing adenosine through purine metabolic pathway. Objectives: The aim of this study was to compare the level of serum ADA with synovial fluid ADA in JIA patients and to see whether it can be utilized as a marker for the activity of the disease Methods: JIA was diagnosed based on International League of Associations for Rheumatology diagnosis criteria. ADA was measured using special kits. Results: 80% of the patients had oligoarticular and 20% polarticulare JIA. There was a significant relation between erythrocyte sedimentation rate (ESR) and high level of synovial fluid ADA. Synovial ADA level was significantly higher than serum ADA in polyarticular JIA. C-reactive protein (CRP), leukocytes and platelets count were increased in high levels of synovial fluid ADA. No correlation was observed between level of serum and synovial fluid ADA. Conclusions: Synovial fluid ADA seems a more precise index than serum ADA to assess the inflammatory condition. In addition, acute-phase response reactants such as ESR, CRP, and platelets count could be suitable predicting parameters for arthritis.

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Evaluation of Serum Adenosine Deaminase Changes Before and After Treatment in Patients with Systemic Lupus Erythematosus, Henoch-Schonlein Purpura and Juvenile Idiopathic Arthritis

Cited by 5 publications

References 28 publications

Characterization of total adenosine deaminase activity (ADA) and its isoenzymes in saliva and serum in health and inflammatory conditions in four different species: an analytical and clinical validation pilot study

Characterization of total adenosine deaminase activity (ADA) and its isoenzymes in saliva and serum in health and inflammatory conditions in four different species: an analytical and clinical validation pilot study

Characterization of total adenosine deaminase activity (ADA) and its isoenzymes in saliva and serum in health and inflammatory conditions to four different species: an analytical and clinical validation pilot study

Comparison of Adenosine Deaminase Level in Serum and Synovial Fluid in Patients with Juvenile Idiopathic Arthritis and Its Relation to Inflammatory Acute Phase Reactants

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