Evaluation of serum anti‐Müllerian hormone concentrations following treatment with vitamin D in Holstein Friesian heifers

Abstract

Anti‐Müllerian hormone (AMH) levels in the blood of dairy cattle are associated with the count of antral follicles (AFC), ovarian function. Numerous studies, particularly in human medicine, have shown that vitamin D3 (Vit D3) has a positive effect on AMH levels. To our knowledge, the effects of Vit D3 on serum AMH levels in heifers have not been investigated. The goal of the current study was to evaluate the concentrations of serum AMH in dairy heifers following Vit D3 treatment. The study included 20 healthy non‐pregnant Holstein Friesian heifers. These animals came to estrus at least once. All heifers received a single intramuscular dosage of 5 million IU Vit D3 (Cholecalciferol). Blood samples were collected from the coccygeal veins of all animals before and after Vit D3 injection (days 7, 14, and 28) for the measurement of AMH and 25(OH)D concentrations. In our analysis, we observed that after exogenous Vit D3 administration, circular 25(OH)D increased constantly (up to day 28 after Vit D3 injection) in all animals. On day 28, AMH concentrations saw a 10% increase in comparison with those measured the day before the Vit D3 injection. However, a comparison of AMH concentrations measured across days did not reveal statistically significant differences between Day 0, 7, 14, and 28 levels (p = .10). Furthermore, no statistical correlation was observed between the pairs (age‐AMH) and (weight‐AMH). Considering all times, no correlation was found between 25(OH)D and blood AMH levels. These findings demonstrated that exogenous Vit D3 did not affect serum AMH in Holstein Friesian heifers.

Comparison of blood ΒHBA measurement devices for diagnosis of subclinical pregnancy toxaemia in sheep: A field study

“…Production traits are affected by congenital diseases (Koca et al, 2024), individual genetic differences (Turgut et al, 2023;, and management (Koca et al, 2023;Komprej et al, 2012) acid profiles (Uztimür et al, 2024), acute phase proteins (Darwish & El-Ebissy, 2019), eosinophil count and eosinophil-based complete blood cell (Gelaw et al, 2022;Tanacan et al, 2023). Together with this, serum or blood BHBA levels are still accepted as a reliable biomarker in the diagnosis of PT in small ruminants.…”

“…Production traits are affected by congenital diseases (Koca et al., 2024), individual genetic differences (Turgut et al., 2023; Turgut & Koca, 2024), and management (Koca et al., 2023; Komprej et al., 2012) in farm animal species. Pregnancy toxaemia is an important metabolic disease that causes economic losses.…”

Elecsys® AMH assay: Determination of Anti‐Müllerian hormone levels and evaluation of the relationship between superovulation response in Holstein dairy cows

“…Electrochemiluminescence immunoassay methodology was used to measure AMH levels. All samples underwent analysis under optimum conditions, utilising the Elecsys AMH automated assay (for Cobas 601 platform, Roche, Germany) as described earlier (Koca et al., 2023 ; Koca et al., 2024 ; Marchetti et al., 2023 ; Turgut & Koca, 2024 ). Before the primary trial, the samples underwent method validation as per the manufacturer's guidelines.…”

“…These measurements were conducted using various assays tailored for specific species: cats: canine AMH ELISA (AL‐116, Ansh Labs, Webster, TX, USA) (Claaßen et al., 2023 ); dogs: AMH Gen II ELISA (A79765, Beckman Coulter‐Immunotech s.r.o., Czechia) (Evci et al., 2023 ) and canine AMH ELISA (AL‐116, Ansh Labs, Webster, TX, USA) (Chotimanukul et al., 2023 ); mares: equine AMH ELISA (AL‐115, Ansh Labs, Webster, TX, USA) (Ucmak et al., 2023 ) and AMH Gen II ELISA (Beckman Coulter, Brea, CA, USA) (Fouché et al., 2022 ); cattle: AMH Bovine Test Kit, Enzyme‐Linked Fluorescent Assay (ELFA) method of the miniVIDAS ® (bioMérieux SA) (Sevgi et al., 2019 ); AMH Gen II ELISA (Beckman Coulter, Brea, CA, USA) (Furukawa et al., 2020 ); bovine AMH ELISA (AnshLabs®, Webster, TX, USA) (Widodo et al., 2022 ). In contrast to these kits, it has been documented that a fully automated Elecsys AMH assay (Roche, 56 for Cobas 601 platform) is utilised for dogs (Ozalp et al., 2023 ), camels (Seyedasgari et al., 2024 ) and heifers (Koca et al., 2023 ). Furthermore, the effectiveness of this kit has been confirmed in research carried out across various species, particularly in women.…”

Anti‐Müllerian hormone: A novel biomarker for detecting bovine freemartinism

“…Serum AMH levels were assessed in blood samples from G1 and G2. The measurement of AMH levels was conducted using the Elecsys AMH kit based on the electrochemiluminescence immunoassay methodology (Roche, Cobas e 601, Germany) (Anckaert et al, 2019;Deeks, 2015;Domain et al, 2022;Jacobs et al, 2019;Koca et al, 2023). Before the primary trial, the samples underwent method validation following the manufacturer's guidelines.…”