Evaluation of serum prestin as a new potential biomarker for hearing damage due to lead exposure in population from Tlaxcala, Mexico

Solis-Angeles, Soledad; Juárez-Pérez, Cuauhtémoc Arturo; Cabello-López, Alejandro; Fascinetto-Dorantes, L.; Amparo, G.; Torres-Valenzuela, Arturo; Aguilar-Madrid, Guadalupe; Razo, Luz María Del

doi:10.1289/isee.2020.virtual.p-0477

Cited by 2 publications

(1 citation statement)

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“…By using a sandwich enzyme-linked immunosorbent assay (ELISA), Prestin was detected in the bloodstream of humans [13][14][15], rats [16,17], guinea pigs [18,19], and mice [20]. Several reports suggested that Prestin in the bloodstream could be used as a biomarker for hearing loss such as idiopathic sudden sensorineural hearing loss [13-15, 21, 22], noise-induced hearing loss [16,17,[23][24][25], sensory hearing loss [26][27][28], age-related hearing loss [29], ototoxic regents induced hearing loss, such as HPβCD [30] and cisplatin [18][19][20]31], and also hearing loss observed in various diseases like Meniere's Disease and Vestibular Migraine [32], COVID-19 [33], lead poison [34], and even surgical related damage [21]. However, the data reported in several studies lack proper negative controls and show inconsistency [13,14].…”

Section: Introductionmentioning

confidence: 99%

Outer Hair Cell Motor Protein, Prestin, Is Not a Reliable Serological Biomarker for Mouse Cochlear Damage

Zheng,

Zhou,

Fuentes

et al. 2024

Preprint

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The motor protein Prestin found in the outer hair cells (OHCs) of the inner ear is responsible for producing high sensitivity and sharp frequency selectivity for mammalian hearing. Some studies have suggested that Prestin could serve as a serological biomarker for cochlear damage since OHCs are highly vulnerable to damage from various sources of insults. However, the reported data are inconsistent and lack appropriate negative controls. To investigate this further, we measured Prestin quantities in the bloodstream using ELISA kits from different companies. Wildtype (WT) mice were exposed to different ototoxic treatments, including noise exposure and ototoxic reagents that kill OHCs rapidly. Prestin-knockout (KO) mice were used as a negative control. Our data show that some ELISA kits were not able to detect Prestin specifically. The ELISA kit that could detect Prestin protein from cochlear homogenates failed to detect Prestin in the bloodstream despite significant damage to OHCs in cochleae. Furthermore, the optical densities of samples, which correlate to Prestin quantities, were significantly influenced by hemolysis in the samples. In conclusion, Prestin from OHCs is not a sensitive and reliable serological biomarker for detecting cochlear damage in mice.

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Section: Introductionmentioning

confidence: 99%

Outer Hair Cell Motor Protein, Prestin, Is Not a Reliable Serological Biomarker for Mouse Cochlear Damage

Zheng,

Zhou,

Fuentes

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Verification of Outer Hair Cell Motor Protein, Prestin, as a Serological Biomarker for Mouse Cochlear Damage

Zheng,

Zhou,

Fuentes

et al. 2024

IJMS

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The motor protein prestin, found in the inner ear’s outer hair cells (OHCs), is responsible for high sensitivity and sharp frequency selectivity in mammalian hearing. Some studies have suggested that prestin could be a serological biomarker for cochlear damage, as OHCs are highly vulnerable to damage from various sources. However, the reported data are inconsistent and lack appropriate negative controls. To investigate whether prestin can be used as a serological biomarker for cochlear damage or stress, we measured prestin quantities in the bloodstreams of mice using ELISA kits from different companies. Wildtype (WT) mice were exposed to different ototoxic treatments, including noise exposure and ototoxic reagents that rapidly kill OHCs. Prestin-knockout (KO) mice were used as a negative control. Our data show that some ELISA kits were not able to detect prestin specifically. The ELISA kit that could detect the prestin protein from cochlear homogenates failed to detect prestin in the bloodstream, despite there being significant damage to OHCs in the cochleae. Furthermore, the optical densities of the serum samples, which correlate to prestin quantities, were significantly influenced by hemolysis in the samples. In conclusion, Prestin from OHCs is not a sensitive and reliable serological biomarker for detecting cochlear damage in mice using ELISA.

show abstract

Evaluation of serum prestin as a new potential biomarker for hearing damage due to lead exposure in population from Tlaxcala, Mexico

Cited by 2 publications

References 0 publications

Outer Hair Cell Motor Protein, Prestin, Is Not a Reliable Serological Biomarker for Mouse Cochlear Damage

Outer Hair Cell Motor Protein, Prestin, Is Not a Reliable Serological Biomarker for Mouse Cochlear Damage

Verification of Outer Hair Cell Motor Protein, Prestin, as a Serological Biomarker for Mouse Cochlear Damage

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