Evaluation of Serum S100A8/S100A9 Levels in Patients with Autoimmune Thyroid Diseases

Korkmaz, Hakan; Tabur, Suzan; Savaş, Esen; Özkaya, Mesut; Aksoy, Şefika; Aksoy, Nurten; Akarsu, Ersin

doi:10.5152/balkanmedj.2016.15881

Cited by 14 publications

(10 citation statements)

References 28 publications

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“…To further explore the potential biological functions of these miRNAs, GO and KEGG enrichment analyses were performed to analyse the NGS data. Upregulated miRNAs were potentially associated with the GO terms myoblast proliferation (associated gene: HGF), CD40 receptor complex (associated gene: TRAF3), leukocyte migration involved in inflammatory response (associated gene: S100A9) and phosphatidylinositol-3,5-bisphosphate binding (associated gene: RAG2), which may participate in the occurrence of HT (22)(23)(24)(25). In addition, Pax-8, which was associated with the GO term 'nephron tubule formation' , participates in the pathogenetic process of AITD by regulating TG and TPO gene expression (26).…”

Section: Discussionmentioning

confidence: 99%

Circulating microRNA Expression Profiling Identifies miR-125a-5p Promoting T Helper 1 Cells Response in the Pathogenesis of Hashimoto's Thyroiditis

et al. 2020

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MicroRNAs (miRNAs) have emerged as key regulators of cellular processes by suppressing target mRNAs at the posttranscriptional level. However, little is known regarding the expression of miRNAs in peripheral blood mononuclear cells (PBMCs) from Hashimoto's thyroiditis (HT) patients. Therefore, 38 HT patients and 36 healthy volunteers were enrolled in this study to identify HT-mediated changes in miRNA expression. Over 1,000 dysregulated miRNAs and their biological functions in the HT patients were identified. Among them, miR-125a-5p expression was upregulated and inversely correlated with low levels of MAF, a transcription factor that inhibits Th1 cells activity and the production of IFN-γ. Luciferase assay results demonstrated that MAF is a direct target gene of miR-125a-5p. Moreover, the proportion of circulating Th1 cells and the transcript levels of IFN-γ were increased in the HT patients. MiR-125a-5p expression positively correlated with the proportion of circulating Th1 cells and the serum concentrations of anti-thyroperoxidase antibodies in the HT patients. Interestingly, knockdown of miR-125a-5p in CD4 + T cells resulted in an elevated level of MAF but decreased the proportion of Th1 cells and the transcript level of IFN-γ in vitro. Furthermore, upregulated miR-125a-5p and IFN-γ transcript levels and downregulated MAF expression were detected in thyroid tissues from HT patients. Receiver operating characteristic (ROC) curves suggested that miR-125a-5p has a crucial role in the HT. Our results demonstrate that the elevated levels of miR-125a-5p contribute to the Th1 cells response in the HT patients and may be involved in the pathogenesis of HT.

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Section: Discussionmentioning

confidence: 99%

Circulating microRNA Expression Profiling Identifies miR-125a-5p Promoting T Helper 1 Cells Response in the Pathogenesis of Hashimoto's Thyroiditis

et al. 2020

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“…S100A9 is a member of S100 protein family, which is involved in in ammatory reaction [10] . S100A9 level in AIT patients was signi cantly higher than that in healthy control group [11] . Compared with AIT-model group, S100A9 mRNA decreased by 0.368 times in the diosgenin-H group (P<0.01), suggesting that diosgenin may slow down the development of AIT by downregulating the expression of S100A9 gene.…”

Section: Discussionmentioning

confidence: 67%

Transcriptome Analysis of the Effect of Diosgenin on Autoimmune Thyroiditis in A Rat Model

Chengfei

Qin

Sun

et al. 2020

Preprint

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Transcriptomics was used to analyze the protective effect of diosgenin on autoimmune thyroiditis (AIT) in rats and its possible mechanism. Forty AIT model rats were established following high iodine induction and injection of porcine thyroglobulin adjuvant into Lewis rats. AIT-model rats were treated with an oral gavage(diosgenin) for 8 weeks. The concentrations of T3, T4, FT3, FT4, TSH, TRAb, TPOAb and TgAb in rat serum were detected. The unilateral thyroid lobes of rats were detected by transcriptomes, RT-qPCR (S100A9, PKA, Creb, Epac, and Rap1 mRNA), and Immunohistochemistry (cAMP, PKA, and Creb). The results showed that the expression of serum T3, T4, FT3, FT4, TSH, TgAb, and TPOAb in the high-dose group was greater than that in the low and middle-dose groups. And compared with the AIT-model group, the relative expression of cAMP, PKA and Creb mRNA and protein increased, S100A9 mRNA decreased in the diosgenin groups. To sum up, diosgenin can improve the expression of T3, T4, FT3, FT4, and TSH in AIT model rats, and decrease the concentrations of TRAb, TgAb and TPOAb, which may be related to activation of the cAMP/PKA/Creb pathway and downregulating the expression of S100A9 gene.

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“…S100A9 is a member of the S100 protein family, which is involved in inflammatory reactions 13 . The S100A9 level in AIT patients has been found to be significantly higher than that in healthy controls 14 . Compared to the AIT-model group, the diosgenin-H group exhibited decreased mRNA S100A9 expression, a level 0.368 times that of the AIT-model group ( P < 0.01), suggesting that diosgenin may slow the development of AIT by downregulating the expression of the S100A9 gene.…”

Section: Discussionmentioning

confidence: 89%

Transcriptome analysis of the effect of diosgenin on autoimmune thyroiditis in a rat model

Chengfei

Qin

Sun

et al. 2021

Sci Rep

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In a mouse model of Graves’ disease (GD), diosgenin has been shown to have a therapeutic effect on GD by alleviating goitre. However, research on the effect of diosgenin on autoimmune thyroiditis (AIT) is lacking. In this study, transcriptomics was used to comprehensively analyse the protective effect of diosgenin against AIT in rats and the possible mechanism. The results showed that in the diosgenin-intervention group, compared to the model group, the expression of serum triiodothyronine, thyroxine, free triiodothyronine, and free thyroxine was decreased and that of thyroid-stimulating hormone was increased; these changes were accompanied by the downregulation of thyroglobulin, TSH receptor antibody and thyroid peroxidase expression in serum. Furthermore, transcriptome detection, RT-qPCR and immunohistochemistry verification revealed that in thyroid tissue, the relative mRNA and protein expression of cyclic adenosine 3′,5′-monophosphate (cAMP), protein kinase A (PKA) and cAMP response element-binding protein (Creb) were increased and the mRNA expression of S100 calcium-binding protein A9 (S100A9) was decreased in the diosgenin groups. In summary, diosgenin alleviates the development of AIT, possibly via the activation of the cAMP/PKA/Creb pathway and downregulation of S100A9 gene expression.

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Evaluation of Serum S100A8/S100A9 Levels in Patients with Autoimmune Thyroid Diseases

Cited by 14 publications

References 28 publications

Circulating microRNA Expression Profiling Identifies miR-125a-5p Promoting T Helper 1 Cells Response in the Pathogenesis of Hashimoto's Thyroiditis

Circulating microRNA Expression Profiling Identifies miR-125a-5p Promoting T Helper 1 Cells Response in the Pathogenesis of Hashimoto's Thyroiditis

Transcriptome Analysis of the Effect of Diosgenin on Autoimmune Thyroiditis in A Rat Model

Transcriptome analysis of the effect of diosgenin on autoimmune thyroiditis in a rat model

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