Evaluation of Serum TNF-alpha, IL-6, IL-10, and IFN-gamma Levels in Patients with Crimean–Congo Hemorrhagic Fever

Abstract

Background Crimean–Congo hemorrhagic fever (CCHF) is a potentially fatal disease caused by a tick-borne virus from the Bunyaviridae family. Cytokines plays an important role in the pathogenesis of viral, bacterial, and immunologic diseases. This study aimed to investigate the role of TNF-alpha, IL-6, IL-10, and IFN-gamma levels in the severity of infection and clinical outcome of patients with CCHF. Methods Patients with confirmed CCHF were divided into two groups (severe cases: Patients who exhibited hemorrhage during their hospital stay, and mild/moderate cases: Patients who displayed no hemorrhage during their hospital stay). Demographic characteristics, laboratory tests on admission of all patients with CCHF were investigated, and serum TNF-alpha, IL-6, IL-10, and IFN-gamma levels were measured. Results A total of 154 patients with confirmed CCHF were investigated. Forty-six (29.9%) of these patients were in the severe group. In patients with severe CCHF, significantly higher serum levels of TNF-alpha (68.2 ± 23.5; P = 0.008) and IL-6 (73.1 ± 41.6; P = 0.003) were detected, compared with cytokine levels in patients who mild/moderate CCHF (Table 1). No differences in serum IL-10 and IFN-gamma levels between patients who severe CCHF and those who mild/moderate CCHF were detected (P > 0.05). Conclusion Cytokines, chemokines, and other inflammatory mediators function in a manner, acting on many different cell types to regulate the host’s immune response. When cytokines present in high concentrations, they might toxic or even lethal effects. In accordance with this view, we have detected increased serum TNF-alpha, IL-6 levels in the patients with severe CCHF. Disclosures All authors: No reported disclosures.

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“…Korva et al, also observed significant differences in IL-10 and GM-CSF between moderate and severe cases (Korva et al, 2019). In another study by Hulva et al, changes in IL-6 and TNF- were noted between moderate and severe cases, but no significant change in IL-8 was observed (Hülya, Yilmaz et al, 2017). Altough IL-6 is often associated with disease severity and fatality, we did not observe any significant difference in IL-6 levels.…”

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“…Korva et al, also observed signi cant differences in IL-10 and GM-CSF between moderate and severe cases 36 . In another study by Hulva et al, changes in IL-6 and TNF-α were noted between moderate and severe cases, but no signi cant change in IL-8 was observed 39 . Although IL-6 is often associated with disease severity and fatality, we did not observe any signi cant difference in IL-6 levels.…”