Low-cost sensors provide an affordable alternative to monitor environmental parameters with acceptable performance. There is a substantial amount of literature where low-cost sensors are compared with high-end reference measurements. However, not all organizations have access to such reference infrastructure. We propose low-cost calibration methods for temperature, relative humidity, and CO2 to allow them to collect their own reliable data. These methods are based on simple techniques and procedures that allow temperature calibration to be achieved in the range of 0 to 50 °C, relative humidity from 0 to 90%, and CO2 between 0 and 1100 ppm. The materials used to create the calibration setups can be purchased online, at hardware stores, and in pharmacies. The reliability of the calibration methods was evaluated using several indicators, such as the airtightness of the calibration box, similarity with the factory calibration, similarity with the reference, similarity between different sensors, replicability of the calibration method, and the similarity with a golden standard. In addition, the results of the low-cost calibration methods were compared with the more advanced calibration methods. A short measurement campaign in the city of Santa Clara, Cuba, demonstrated that such calibrations transform in-house developed monitoring systems into valid low-cost scientific instruments for decision-making. This work creates opportunities for institutions and researchers hosted in low- and mid-income countries to build and validate their own equipment to reliably solve local problems.