Evaluation of Silver Ion-Releasing Scaffolds in a 3D Coculture System of MRSA and Human Adipose-Derived Stem Cells for Their Potential Use in Treatment or Prevention of Osteomyelitis

Abstract

Bone infection, also called osteomyelitis, can result when bacteria invade a bone. Treatment of osteomyelitis usually requires surgical debridement and prolonged antimicrobial therapy. The rising incidence of infection with multidrug-resistant bacteria, in particular methicillin-resistant staphylococcus aureus (MRSA), however, limits the antimicrobial treatment options available. Silver is well known for its antimicrobial properties and is highly toxic to a wide range of microorganisms. We previously reported our development of biocompatible, biodegradable, nanofibrous scaffolds that released silver ions in a controlled manner. The objective of this study was to determine the efficacy of these scaffolds in treating or preventing osteomyelitis. To achieve this objective, antimicrobial efficacy was determined using a 3D coculture system of human adipose-derived stem cells (hASC) and MRSA. Human ASC were seeded on the scaffolds and induced to undergo osteogenic differentiation in both the absence and presence of MRSA. Our results indicated that the silver ion-releasing scaffolds not only inhibited biofilm formation, but also supported osteogenesis of hASC. Our findings suggest that these biocompatible, degradable, silver ion-releasing scaffolds can be used at an infection site to treat osteomyelitis and/or to coat bone implants as a preventative measure against infection postsurgery.

Effects of electrical biostimulation and silver ions on porcine fibroblast cells

“…Interestingly, the fibroblasts treated with approximately 2.00 mg/L of electrically generated silver ions in our experiments exhibited cellular biochemical characteristics that are similar to the burst of oxidative phosphorylation seen during early cell reprogramming [80]. Our results suggest that the potential of silver ions inducing an oxidative phosphorylation burst is possible and could provide a supplemental explanation to the studies that showed silver ions can promote tissue regeneration and wound healing while preventing bacterial infection [9,[81][82][83].…”

Adipose-Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post-Traumatic Osteomyelitis and Modulate B-Cell Response

“…In this context, the immunomodulatory and antibacterial abilities of MSCs could be used as a new and innovative approach treating bacterial infections and autoimmune disorders like graft versus host disease [26]. More specifically, ASCs showed beneficial effects preventing and treating osteomyelitis [27] and could restore bone regeneration of osteonecrosis in maxillofacial surgery [28].…”

Evaluation of the temporary effect of physical vapor deposition silver coating on resistance to infection in transdermal skin and bone integrated pylon with deep porosity

“…Matrix metalloproteinase‐1 (MMP‐1) (collagenase‐1) is a member of the family of zinc‐dependent endopeptidases known to be a key regulator of epithelial cell migration indicating its role in re‐epithelialization . MMP‐1 also plays role in osteogenesis and muscle regeneration by improving the differentiation and migration of myoblasts, and promotes myogenic differentiation of bone marrow‐derived mesenchymal stem cells (BMSCs) . Zhang et al demonstrated that silver nanoparticles were efficient in promoting osteoblastic differentiation in both in vitro and in vivo studies .…”