Evaluation of Silymarin as a Promising Radioprotector

Adhikari, Manish; Arora, Rajesh; Chawla, Raman; Sharma, Jyoti; Dhaker, Atlar Singh; Gupta, Damodar; Dubey, Neha; Kumar, Raj; Иванов, Веселин; Gadjeva, V.; Gevrenova, Reneta; Sharma, Rakesh

doi:10.1515/znc-2010-5-605

Cited by 15 publications

(11 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…57 The radioprotective property of Podophyllum hexandrum fraction (REC-2006) in p53-carrying hepatoma cell line, perhaps mediated by cell cycle regulatory proteins 58 and free radical scavenging activity and radioprotection of Caesalpinia digyna extracts 59 has been reported. More recently, the radioprotective property of a seed extract of Silymarin, with INM-7035 as its bioactive constituent 60 and the radiation mitigating effect of hydro-alcoholic extract of Saraca indica (SIE) against mice exposed to whole body γ-radiation have also been reported. 61 The exact mechanism by which SCE protects against the genotoxic effects of radiation is not known.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Radiation-Induced DNA Damage by Jamun, Syzygium cumini, in the Cultured Splenocytes of Mice Exposed to Different Doses of γ-Radiation

2011

View full text Add to dashboard Cite

The radioprotective property of 50 mg/kg body weight jamun (Syzygium cumini) extract was studied in the cultured splenocytes of mice exposed to 0, 0.5, 1, 2, 3, or 4 Gy of γ-radiation. The spleens of irradiated mice were removed aseptically and the splenocytes were extracted from the individual spleens and cultured. The micronuclei were prepared 72 hours after irradiation in binucleate splenocytes by blocking cytokinesis with cytochalasin-B. Irradiation of mice resulted in a dose-dependent elevation in the micronucleated splenocytes. The exposure of mice not only elevated splenocytes bearing one micronucleus but also cells bearing 2 and multiple (>2) micronuclei indicating induction of complex DNA damage after irradiation. Oral treatment of mice with 50 mg/kg body weight of jamun leaf extract protected against the radiation-induced micronuclei formation. Jamun extract also protected against the formation of 2 and multiple micronuclei indicating repair or inhibition of complex DNA damage. The assessment of lipid peroxidation in mice brain homogenate has indicated a concentration dependent inhibition of lipid peroxidation by jamun extract. Studies in a cell free system revealed that jamun extract inhibited the formation of OH, O 2 −, DPPH, and ABTS + free radicals in a concentration dependent manner. Our study demonstrates that jamun extract protected mice against the radiation-induced DNA damage and inhibition of radiation-induced free radical formation may be one of the mechanisms of radioprotection.

show abstract

Section: Discussionmentioning

confidence: 99%

Inhibition of Radiation-Induced DNA Damage by Jamun, Syzygium cumini, in the Cultured Splenocytes of Mice Exposed to Different Doses of γ-Radiation

2011

View full text Add to dashboard Cite

show abstract

“…Silymarin (10) is frequently used in treatment of liver diseases for protection of liver cells against lipid peroxidation and prevention of liver glutathione depletion (Valenzuela et al, 1989;Mira et al, 1994). Several investigations have reported that silymarin (10) alleviated irradiation-induced damage, including changes in nucleic acids and histone proteins, and inhibited radiation-induced free radical generation and lipid peroxidation (Gakova et al, 1992;Adhikari et al, 2010). Silymarin (10) treatment resulted in significant protection against radiation-induced hepatotoxicity in liver of rats by antioxidant and free radical scavenging properties of silymarin (10) (Ramadan et al, 2002).…”

Section: Silymarinmentioning

confidence: 99%

Phenylpropanoids in radioregulation: double edged sword

2011

View full text Add to dashboard Cite

Radiotherapy, frequently used for treatment of solid tumors, carries two main obstacles including acquired radioresistance in cancer cells during radiotherapy and normal tissue injury. Phenylpropanoids, which are naturally occurring phytochemicals found in plants, have been identified as potential radiotherapeutic agents due to their anti-cancer activity and relatively safe levels of cytotoxicity. Various studies have proposed that these compounds could not only sensitize cancer cells to radiation resulting in inhibition of growth and cell death but also protect normal cells against radiation-induced damage. This review is intended to provide an overview of recent investigations on the usage of phenylpropanoids in combination with radiotherapy in cancer treatment.

show abstract

“…Approximately 95 percent of colorectal cancers are adenocarcinomas. Silymarin has already been evidentiated for its radioprotective efficacy both at in vitro and in vivo levels [2][3][4][5] . The anticancer effects of silymarin can be understood by various molecular mechanisms including blocking of carcinogenesis at different stages, such as initiation, promotion, and progression.…”

Section: Introductionmentioning

confidence: 99%

Studies on radiation sensitization efficacy by silymarin in colon carcinoma cells

Lal

Gupta

2016

Discoveries (Craiova)

Self Cite

View full text Add to dashboard Cite

Recent reports demonstrated the role of silymarin as a cytoprotective agent for normal cells against ionizing or non-ionizing (UV) radiation, and in inhibiting the chemically initiated or promoted carcinogenesis in several malignancies, such as skin or prostate cancers. Silymarin is a plant flavonoid obtained from milk thistle; the main active principles in milk thistle are silybin (silibinin), sylichrisitin and silydianin, commonly referred as silymarin. In the present study, we aimed to investigate the radiation modulatory effects of silymarin on cancer cells. For this, we used the HCT-15 and RKO colon cancer cell lines as a model. Pre-irradiation treatment of cells with silymarin (20 µg/ml) followed by radiation exposure inhibits colon cancer cell proliferation and enhances cell death in a time-dependent manner. We have also examined the changes in p53 phosphorylation at Ser15, phosphorylation of p38 and their association with DNA damage. Silymarin was found to reduce proliferation of the human colon carcinoma cells in a concentration and timedependent manner. Moreover, percentage of cell death was also increased in combined treatment (20µg/ml of silymarin + radiation). Our studies indicate that the combination increases the arrest of cells in G 2 /M phase of cell cycle, DNA damageinduced decrease in mitochondrial membrane potential (MMP) and a decrease of the reactive oxygen species (ROS) levels, which are associated with an increase in cell death. Altogether, these results suggest that silymarin sensitizes colon cancer cells to radiation, strategy with potential for colon cancer treatment. Noteworthy, since silymarin was previously shown to confer protection against radiation in at least some types of normal tissues, additional studies are needed to further investigate the potential of silymarin in colon cancer therapy when combined with radiation, its potential protective effects on normal tissues and its mechanisms of action.

show abstract

Evaluation of Silymarin as a Promising Radioprotector

Cited by 15 publications

References 35 publications

Inhibition of Radiation-Induced DNA Damage by Jamun, Syzygium cumini, in the Cultured Splenocytes of Mice Exposed to Different Doses of γ-Radiation

Inhibition of Radiation-Induced DNA Damage by Jamun, Syzygium cumini, in the Cultured Splenocytes of Mice Exposed to Different Doses of γ-Radiation

Phenylpropanoids in radioregulation: double edged sword

Studies on radiation sensitization efficacy by silymarin in colon carcinoma cells

Contact Info

Product

Resources

About